Pluronic F-127 Hydrogel Loaded with Human Adipose-Derived Stem Cell-Derived Exosomes Improve Fat Graft Survival via HIF-1α-Mediated Enhancement of Angiogenesis

doi:10.2147/IJN.S435106

科研成果详情

题名	Pluronic F-127 Hydrogel Loaded with Human Adipose-Derived Stem Cell-Derived Exosomes Improve Fat Graft Survival via HIF-1α-Mediated Enhancement of Angiogenesis
作者	Yang, Fangfang1; Li, Liqun1; Li, Zihao 2; Cai, Zhongming1; He, Yucang1; Ke, Chen1; Wang, Jingping 1; Lin, Ming3
发表日期	2023
发表期刊	INTERNATIONAL JOURNAL OF NANOMEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	autologous fat grafting human adipose-derived stem cell exosome hydrogel HIF-1 alpha/VEGF signaling pathway angiogenesis
其他关键词	TRANSPLANTATION ; MIR-126-3P ; REPAIR
摘要	Purpose: Autologous fat grafting is playing an increasingly important role in plastic surgery. However, high absorption and low survival of autologous fat grafts limit their clinical application. This study aimed to investigate whether human adipose-derived stem cell-derived exosomes (hASC-Exos) encapsulated in a PF-127 hydrogel can improve the survival of autologous fat grafts and to elucidate the underlying mechanisms.Patients and Methods: Exosomes were isolated from hASCs and identified using transmission electron microscopy, nanoparticle tracking analysis and Western blotting. We performed functional assays in vitro to assess the effect of hASC-Exos on proliferation, migration, and tube formation as well as their regulatory role in the HIF-1 alpha/VEGF signaling pathway. hASC-Exos encapsulated in the PF-127 hydrogel were used as an in vivo autologous fat graft model. The effects of the PF-127 hydrogel/hASC-Exos and the role of the HIF-1 alpha/VEGF signaling pathway in promoting angiogenesis in an autologous fat grafting model were assessed.Results: hASC-Exos were taken up by human umbilical vein endothelial cells and enhanced their proliferation, migration, and tubule formation in vitro. The effects of hASC-Exos on promoting angiogenesis were mediated by the HIF-1 alpha/VEGF signaling pathway. Moreover, we fabricated a PF-127 hydrogel for the sustained release of hASC-Exos, and in vivo results showed that hASC-Exos encapsulated in PF-127 hydrogel improved the survival of autologous fat grafts.Conclusion: Our findings indicated that hASC-Exos encapsulated in PF-127 hydrogel serve as a key regulator of angiogenesis by activating the HIF-1 alpha/VEGF signaling pathway and provide a promising strategy for autologous fat grafting treatment.
资助项目	National Natural Science Foundation of China [81971850]
出版者	DOVE MEDICAL PRESS LTD
ISSN	1178-2013
EISSN	1178-2013
卷号	18 页码:6781-6796
DOI	10.2147/IJN.S435106
页数	16
WOS类目	Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
WOS记录号	WOS:001107578100001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	38026529
SCOPUSEID	2-s2.0-85177052931
通讯作者地址	[Li, Liqun]Department of Plastic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
Scopus学科分类	Biophysics;Bioengineering;Biomaterials;Pharmaceutical Science;Drug Discovery;Organic Chemistry
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/205536
专题	第一临床医学院（信息与工程学院）、附属第一医院_外科学_整形外科附属第二医院附属第一医院第二临床医学院、附属第二医院、育英儿童医院附属第二医院_妇产科
通讯作者	Li, Liqun
作者单位	1.Department of Plastic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Department of Wenzhou Medical University,Zhejiang,Wenzhou,China; 3.Department of Obstetrics and Gynecology,Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Yang, Fangfang,Li, Liqun,Li, Zihao,et al. Pluronic F-127 Hydrogel Loaded with Human Adipose-Derived Stem Cell-Derived Exosomes Improve Fat Graft Survival via HIF-1α-Mediated Enhancement of Angiogenesis[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2023,18:6781-6796.
APA	Yang, Fangfang., Li, Liqun., Li, Zihao., Cai, Zhongming., He, Yucang., ... & Lin, Ming. (2023). Pluronic F-127 Hydrogel Loaded with Human Adipose-Derived Stem Cell-Derived Exosomes Improve Fat Graft Survival via HIF-1α-Mediated Enhancement of Angiogenesis. INTERNATIONAL JOURNAL OF NANOMEDICINE, 18, 6781-6796.
MLA	Yang, Fangfang,et al."Pluronic F-127 Hydrogel Loaded with Human Adipose-Derived Stem Cell-Derived Exosomes Improve Fat Graft Survival via HIF-1α-Mediated Enhancement of Angiogenesis".INTERNATIONAL JOURNAL OF NANOMEDICINE 18(2023):6781-6796.