Inhibition of human and rat 3 beta-hydroxysteroid dehydrogenase and 17 beta-hydroxysteroid dehydrogenase 3 activities by perfluoroalkylated substances

doi:10.1016/j.cbi.2010.07.001

科研成果详情

题名	Inhibition of human and rat 3 beta-hydroxysteroid dehydrogenase and 17 beta-hydroxysteroid dehydrogenase 3 activities by perfluoroalkylated substances
作者	Zhao, Binghai 1,2; Hu, Guo-Xin3; Chu, Yanhui 2; Jin, Xiudong 2; Gong, Shouliang 4; Akingbemi, Benson T.5; Zhang, Zhiqiang6; Zirkin, Barry R.7; Ge, Ren-Shan1,6
发表日期	2010-10-06
发表期刊	CHEMICO-BIOLOGICAL INTERACTIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	PFOS Perfluoroalkylated substances Human Rat Enzyme inhibition 3 beta-Hydroxysteroid dehydrogenase 17 beta-Hydroxysteroid dehydrogenase 3
其他关键词	PEROXISOME PROLIFERATION ; PERFLUOROOCTANOIC ACID ; MODULATION ; EXPOSURE
摘要	Perfluoroalkylated substances (PFASs) including perfluorooctane acid (PFOA) and perfluorooctane sulfonate (PFOS) have been classified as persistent organic pollutants and are known to cause reduced testosterone production in human males. The objective of the present study was to compare the potencies of five different PFASs including PFOA. PFOS, potassium perfluorooctane sulfonate (PFOSK), potassium perfluorohexane sulfonate (PFHxSK) and potassium perfluorobutane sulfonate (PFBSK) in the inhibition of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 17 beta-hydroxysteroid dehydrogenase 3 (17 beta-HSD3) activities in the human and rat testes. Human and rat microsomal enzymes were exposed to various PFASs. PFOS and PFOSK inhibited rat 3 beta-HSD activity with IC50 of 1.3.5 +/- 0.05 and 1.77 +/- 0.04 mu M, respectively, whereas PFHxSK and PFBSK had no effect at concentrations up to 250 mu M. All chemicals tested weakly inhibited human 3 beta-HSD activity with IC(50)s over 250 mu M. On the other hand, PFOS. PFOSK and PFOA inhibited human 17 beta-HSD3 activity with IC(50)s of 6.02 +/- 1.02, 4.39 +/- 0.46 and 127.60 +/- 28.52 mu M, respectively. The potencies for inhibition of 17 beta-HSD3 activity were determined to be PFOSK > PFOS> PFOA> PFHxSK = PFBSK for human 17 beta-HSD3 activity. There appears to be a species-dependent sensitivity to PFAS-mediated inhibition of enzyme activity because the IC(50)s of PFOS(K) for inhibition of rat 17 beta-HSD3 activity was greater than 250 mu M. In conclusion, the present study shows that PFOS and PFOSK are potent inhibitors of rat 3 beta-HSD and human 17 beta-HSD3 activity, and implies that inhibition of steroidogenic enzyme activity may be a contributing factor to the effects that PFASs exert on androgen secretion in the testis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
资助项目	Population Council Fellowship; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30871434]
出版者	ELSEVIER IRELAND LTD
出版地	CLARE
ISSN	0009-2797
EISSN	1872-7786
卷号	188 期号:1 页码:38-43
DOI	10.1016/j.cbi.2010.07.001
页数	6
WOS类目	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:000282391400005
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	20619251
SCOPUSEID	2-s2.0-77956174680
通讯作者地址	[Zhang, Zhiqiang]2nd Affiliated Hospital,Wenzhou Medical College,China
Scopus学科分类	Toxicology
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/20201
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院_生殖与药物内分泌研究所
通讯作者	Zhang, Zhiqiang
作者单位	1.Population Council,1230 York Avenue,United States; 2.Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy,Mudanjiang Medical University,China; 3.Institute for Reproductive Medicine and Endocrinology,Wenzhou Medical College,China; 4.School of Public Health,Jilin University,China; 5.Department of Anatomy, Physiology and Pharmacology,Auburn University,United States; 6.2nd Affiliated Hospital,Wenzhou Medical College,China; 7.Department of Biochemistry and Molecular Biology,The Johns Hopkins University Bloomberg School of Public Health,United States
通讯作者单位	温州医科大学
推荐引用方式 GB/T 7714	Zhao, Binghai,Hu, Guo-Xin,Chu, Yanhui,et al. Inhibition of human and rat 3 beta-hydroxysteroid dehydrogenase and 17 beta-hydroxysteroid dehydrogenase 3 activities by perfluoroalkylated substances[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2010,188(1):38-43.
APA	Zhao, Binghai., Hu, Guo-Xin., Chu, Yanhui., Jin, Xiudong., Gong, Shouliang., ... & Ge, Ren-Shan. (2010). Inhibition of human and rat 3 beta-hydroxysteroid dehydrogenase and 17 beta-hydroxysteroid dehydrogenase 3 activities by perfluoroalkylated substances. CHEMICO-BIOLOGICAL INTERACTIONS, 188(1), 38-43.
MLA	Zhao, Binghai,et al."Inhibition of human and rat 3 beta-hydroxysteroid dehydrogenase and 17 beta-hydroxysteroid dehydrogenase 3 activities by perfluoroalkylated substances".CHEMICO-BIOLOGICAL INTERACTIONS 188.1(2010):38-43.