血必净注射液对硫化氢急性中毒大鼠肺组织细胞因子及核转录因子-κB 的影响

doi:10.3969/j.issn.1008-9691.2012.06.009

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题名	血必净注射液对硫化氢急性中毒大鼠肺组织细胞因子及核转录因子-κB 的影响
其他题名	Effect of Xuebijing injection on cytokines and nuclear factor-κB in lung tissue of acute hydrogen sulfide- intoxicated rats
作者	卢晗 1; 葛赟 1; 洪广亮1; 姜小珍 1; 刘瑶 1; 邱俏檬1; 卢中秋1
发表日期	2012
发表期刊	中国中西医结合急救杂志影响因子和分区
语种	中文
原始文献类型	Periodical
关键词	硫化氢血必净注射液肺损伤急性核转录因子-κB 肿瘤坏死因子-α 白细胞介素
其他关键词	Hydrogen sulfide ; Xuebijing injection ; Acute lung injury ; Nuclear factor-κB ; Tumor necrosis factor-α ; Interleukin
摘要	目的探讨血必净注射液对硫化氢(H2S)急性中毒大鼠肺组织肿瘤坏死因子-α(TNF-α)、白细胞介素(IL-6、IL-10)及核转录因子-κB(NF-κB)的影响.方法将96只清洁级 SD 大鼠按随机数字表法分为正常对照组(n =8)、血必净对照组(n =8)、H2S 染毒模型组(n =40)和血必净干预组(n =40).采用酶联免疫吸附试验(ELISA)测定肺组织中 TNF-α、IL-6、IL-10水平;用逆转录-聚合酶链反应(RT-PCR)和蛋白质免疫印迹法(Western blotting)检测肺组织 NF-κB mRNA 和蛋白表达;光镜下观察肺组织病理学改变.结果正常对照组和血必净对照组 TNF-α、IL-6、IL-10水平及 NF-κB mRNA 和蛋白表达比较差异均无统计学意义(均P0.05).与正常对照组比较,H2S 染毒模型组在染毒后2、6、12、24、48 h TNF-α(ng/L)、IL-6(ng/L)、IL-10(ng/L)水平及 NF-κB mRNA 和蛋白表达明显升高(均P<0.01),分别于12、48、48、24、24 h 达峰值(TNF-α12 h:400.79±13.74,IL-648 h :242.69±8.96,IL-1048 h :353.73±10.75,NF-κB mRNA 24 h :0.568±0.007,NF-κB蛋白24 h :0.520±0.007);与 H2S 染毒模型组同期比较,血必净干预组 TNF-α、IL-6水平及 NF-κB mRNA和蛋白于2 h 开始即明显降低(TNF-α:322.46±10.16比377.89±10.87,IL-6:124.08±8.14比170.54±8.25, NF-κB mRNA :0.151±0.005比0.274±0.006,NF-κB 蛋白:0.109±0.005比0.309±0.006,均 P<0.01),IL-10水平明显升高(361.94±11.04比321.80±13.86,P<0.01).光镜下可见24 h 时血必净干预组肺损伤程度明显减轻.结论血必净注射液干预能显著降低促炎因子 TNF-α、IL-6水平及 NF-κB mRNA 和蛋白的表达,提高抗炎因子 IL-10水平,减轻急性肺损伤.
其他摘要	Objective To investigate the effect of Xuebijing injection on the levels of tumor necrosis factor-α(TNF-α),interleukin(IL-6,IL-10)and nuclear factor-κB(NF-κB)in the lung tissue of acute hydrogen sulfide (H2S)-intoxicated rats. Methods A total of 96 Sprague-Dawley(SD)rats of clean grade were divided randomly into four groups :normal control group(n =8),Xuebijing control group(n =8),H2S-intoxicated model group(n = 40) and Xuebijing treatment group(n = 40). After modeling,the levels of TNF-α,IL-6 and IL-10 in the lung tissue were measured by enzyme-linked immunosorbent assay(ELISA)methods,and the expression of NF-κB mRNA and protein in the lung tissue was detected by reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting methods. Pathological changes of lung tissue were observed by light microscope. Results There was no significant difference between normal control group and Xuebijing control group in the levels of TNF-α,IL-6 and IL-10 and the expression of NF-κB mRNA and protein(all P0.05). The TNF-α(ng/L),IL-6(ng/L)and IL-10(ng/L) levels,and NF-κB mRNA and protein expression at 2,6,12,24,48 hours in rats of H2S-intoxicated model group after modeling were markedly increased than those in normal control group(all P<0.01);they reached the peak at 12, 48,48,24,24 hours respectively(TNF-α 12 hours :400.79±13.74,IL-6 48 hours :242.69±8.96,IL-10 48 hours :353.73±10.75,NF-κB mRNA 24 hours:0.568±0.007,NF-κB protein 24 hours:0.520±0.007). In comparison with the same period of H2S-intoxicated model group,the TNF-α,IL-6 levels and NF-κB mRNA and protein expression at 2 hours in rats of Xuebijing treatment group decreased significantly(TNF-α:322.46±10.16 vs. 377.89±10.87, IL-6:124.08±8.14 vs. 170.54±8.25,NF-κB mRNA:0.151±0.005 vs. 0.274±0.006,NF-κB protein:0.109±0.005 vs. 0.309±0.006,all P<0.01),and the IL-10 levels at 2 hours increased significantly(361.94±11.04 vs. 321.80±13.86,P<0.01)in the Xuebijing treatment group. And at 24 hours after modeling,the degree of lung damage was also decreased in rats of Xuebijing treatment group under the light microscope. Conclusion The interference of Xuebijing injection can significantly inhibit the levels of cytokines,TNF-α and IL-6,the expression of NF-κB mRNA and protein,and up-regulate the level of IL-10,the anti-inflammatory factor,resulting in reduction of the degree of acute lung injury in rats.
ISSN	1008-9691
期号	6 页码:343-347
DOI	10.3969/j.issn.1008-9691.2012.06.009
页数	0
收录类别	万方 ; ISTIC ; 北大核心
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引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/201197
专题	第二临床医学院，附属第二医院、育英儿童医院附属第一医院_急诊科
作者单位	1.温州医学院第二临床医学院,浙江温州 325035; 2.温州医学院附属第一医院急诊科,浙江温州 325000
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	卢晗,葛赟,洪广亮,等. 血必净注射液对硫化氢急性中毒大鼠肺组织细胞因子及核转录因子-κB 的影响[J]. 中国中西医结合急救杂志,2012(6):343-347.
APA	卢晗., 葛赟., 洪广亮., 姜小珍., 刘瑶., ... & 卢中秋. (2012). 血必净注射液对硫化氢急性中毒大鼠肺组织细胞因子及核转录因子-κB 的影响. 中国中西医结合急救杂志(6), 343-347.
MLA	卢晗,et al."血必净注射液对硫化氢急性中毒大鼠肺组织细胞因子及核转录因子-κB 的影响".中国中西医结合急救杂志 .6(2012):343-347.