Isolation of Exosome Nanoparticles from Human Cerebrospinal Fluid for Proteomic Analysis

doi:10.1021/acsanm.0c02622

科研成果详情

题名	Isolation of Exosome Nanoparticles from Human Cerebrospinal Fluid for Proteomic Analysis
作者	Li, Meng1,2,3,4; Huang, Liu 5; Chen, Joyce 6; Ni, Fangfang 2; Zhang, Yating 4; Liu, Fei1,2,3
发表日期	2021-04-23
发表期刊	ACS APPLIED NANO MATERIALS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	cerebrospinal fluid exosomes purification and enrichment proteomics diagnostics
其他关键词	CANCER ; RNA
摘要	Exosomes are nanoscale (30-150 nm) biological vesicles that are actively released from living cells and circulating into all body fluids. Recently, exosomes in cerebrospinal fluid (CSF) have been recognized as promising biomarkers for central nervous system (CNS) neoplasms. In this study, we report a label-free method that can be used to rapidly isolate exosomes from CSF for proteomic analysis. Compared to ultracentrifugation and polyethylene glycol-based precipitation, our method isolates exosomes from 2 mL of CSF within 10 min, which is 18 times and 72 times shorter, respectively; the yield was increased by 4.47 times and 2.09 times and the purity was increased by 4.54 times and 9.76 times, respectively. The proteomic analysis further revealed that the exosomes isolated by our method identified more exosome-related proteins, which may reflect the physiological status of diseases for exosome-based diagnosis. Therefore, the effective isolation of pure exosomes from CSF samples for protein analysis will benefit the downstream analysis and clinical translation of exosomes, thus promoting the early diagnosis of CNS neoplasms.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [U1703251, U1810113]; Zhejiang Provincial and Ministry of Health Research Fund for Medical Sciences [WKJ-ZJ-1910]; Wenzhou Medical University [89218012]; Wenzhou Institute of Biomaterials Engineering [WIBEZD2017006-05]; Shanxi Provincial Innovation Capability Support Program [2019TD-021]
出版者	AMER CHEMICAL SOC
出版地	WASHINGTON
ISSN	2574-0970
EISSN	2574-0970
卷号	4 期号:4 页码:3351-3359
DOI	10.1021/acsanm.0c02622
页数	22
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS记录号	WOS:000644473900008
收录类别	SCIE ; EI ; SCOPUS
EI入藏号	20211510199823
EI主题词	Cerebrospinal fluid
URL	查看原文
SCOPUSEID	2-s2.0-85103780934
通讯作者地址	[Liu, Fei]First Affiliated Hospital,Wenzhou Medical University,Wenzhou, Zhejiang,325035,China ; [Zhang, Yating]College of Chemistry and Chemical Engineering,Xi'An University of Science and Technology,Xi'an,710054,China
Scopus学科分类	Materials Science (all)
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/19745
专题	附属第一医院
通讯作者	Zhang, Yating; Liu, Fei
作者单位	1.First Affiliated Hospital,Wenzhou Medical University,Wenzhou, Zhejiang,325035,China; 2.Eye Hospital,School of Ophthalmology and Optometry,School of Biomedical Engineering,Wenzhou Medical University,Wenzhou, Zhejiang,325035,China; 3.Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou, Zhejiang,325001,China; 4.College of Chemistry and Chemical Engineering,Xi'An University of Science and Technology,Xi'an,710054,China; 5.Department of Oncology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China; 6.Department of Bioengineering,University of California,Berkeley,Berkeley,94720,United States
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Li, Meng,Huang, Liu,Chen, Joyce,et al. Isolation of Exosome Nanoparticles from Human Cerebrospinal Fluid for Proteomic Analysis[J]. ACS APPLIED NANO MATERIALS,2021,4(4):3351-3359.
APA	Li, Meng, Huang, Liu, Chen, Joyce, Ni, Fangfang, Zhang, Yating, & Liu, Fei. (2021). Isolation of Exosome Nanoparticles from Human Cerebrospinal Fluid for Proteomic Analysis. ACS APPLIED NANO MATERIALS, 4(4), 3351-3359.
MLA	Li, Meng,et al."Isolation of Exosome Nanoparticles from Human Cerebrospinal Fluid for Proteomic Analysis".ACS APPLIED NANO MATERIALS 4.4(2021):3351-3359.