Pure curcumin decreases the expression of WT1 by upregulation of miR-15a and miR-16-1 in leukemic cells

doi:10.1186/1756-9966-31-27

科研成果详情

题名	Pure curcumin decreases the expression of WT1 by upregulation of miR-15a and miR-16-1 in leukemic cells
作者	Gao, Shen-meng2; Yang, Jun-jun3; Chen, Chi-qi 2; Chen, Jun-jie2; Ye, Li-ping2; Wang, Lu-yao 2; Wu, Jian-bo2; Xing, Chong-yun1; Yu, Kang1
发表日期	2012-03-27
发表期刊	JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Curcumin WT1 miR-15a miR-16-1
其他关键词	MINIMAL RESIDUAL DISEASE ; GENE-EXPRESSION ; MICRORNAS ; PROLIFERATION ; SUPPRESSOR ; TARGET ; TOOL ; PCR
摘要	Background: Pure curcumin has been reported to down-regulate the expression of WT1 in leukemic cells. However, the molecular mechanism underlying the down-regulation of WT1 by curcumin is not completely delineated. The purpose of this present study is to identify a new miRNA-mediated mechanism which plays an important role in the anti-proliferation effects of curcumin in leukemic cells. Methods: K562 and HL-60 cells were treated with different concentrations of curcumin for 24 and 48 hours, the level of miR-15a/16-1 and WT1 were detected by qRT-PCR and Western blotting. WT1 expression and cell proliferation were detected by Western blotting and CCK-8, after curcumin treated-K562 and HL-60 cells were transfected with anti-miR-15a/16-1 oligonucleotides. Results: We found that pure curcumin upregulated the expression of miR-15a/16-1 and downregulated the expression of WT1 in leukemic cells and primary acute myeloid leukemia (AML) cells. Overexpression of miR-15a/16-1 deduced the protein level of WT1 in leukemic cells, but downregulation of WT1 by siRNA-WT1 could not increase the expression of miR-15a/16-1 in leukemic cells. These results reveal that curcumin induced-upregulation of miR-15a/16-1 is an early event upstream to downregulation of WT1. Furthermore, anti-miR-15a/16-1 oligonucleotides (AMO) partly reversed the downregulation of WT1 induced by pure curcumin in leukemic cells and AMO promoted the growth of curcumin treated-K562 and HL-60 cells. Conclusion: Thus, these data suggest for the first time that pure curcumin downregulated the expression of WT1 partly by upregulating the expression of miR-15a/16-1 in leukemic cells. miR-15a/16-1 mediated WT1 downregulation plays an important role in the anti-proliferation effect of curcumin in leukemic cells.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81172613]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2101069, Y206383, Y12H080019]; Scientifical Research Foundation of Zhejiang Provincial Education Department [Y201119952]
出版者	BMC
出版地	LONDON
ISSN	1756-9966
EISSN	1756-9966
卷号	31 期号:1 页码:27
DOI	10.1186/1756-9966-31-27
页数	9
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000302803500001
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	22449094
PMC记录号	PMC3325897
通讯作者地址	[Yu, Kang]Wenzhou Med Coll, Affiliated Hosp 1, Dept Hematol, 2 FuXue Rd, Wenzhou 325000, Peoples R China.
TOP期刊	TOP期刊
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/19229
专题	附属第一医院附属第二医院第二临床医学院，附属第二医院、育英儿童医院附属第二医院_药物临床试验办公室
通讯作者	Yu, Kang
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 1, Dept Hematol, Wenzhou 325000, Peoples R China; 2.Wenzhou Med Coll, Affiliated Hosp 1, Lab Internal Med, Wenzhou 325000, Peoples R China; 3.Wenzhou Med Coll, Affiliated Hosp 2, Clin Lab, Wenzhou 325000, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Gao, Shen-meng,Yang, Jun-jun,Chen, Chi-qi,et al. Pure curcumin decreases the expression of WT1 by upregulation of miR-15a and miR-16-1 in leukemic cells[J]. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,2012,31(1):27.
APA	Gao, Shen-meng., Yang, Jun-jun., Chen, Chi-qi., Chen, Jun-jie., Ye, Li-ping., ... & Yu, Kang. (2012). Pure curcumin decreases the expression of WT1 by upregulation of miR-15a and miR-16-1 in leukemic cells. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 31(1), 27.
MLA	Gao, Shen-meng,et al."Pure curcumin decreases the expression of WT1 by upregulation of miR-15a and miR-16-1 in leukemic cells".JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 31.1(2012):27.