Comprehensive Analysis to Identify MAGEA3 Expression Correlated With Immune Infiltrates and Lymph Node Metastasis in Gastric Cancer

doi:10.3389/fonc.2021.784925

科研成果详情

题名	Comprehensive Analysis to Identify MAGEA3 Expression Correlated With Immune Infiltrates and Lymph Node Metastasis in Gastric Cancer
作者	Jin, Jinji 1; Tu, Jianxin2; Ren, Jiahuan 1; Cai, Yiqi1; Chen, Wenjing 1; Zhang, Lifang 3; Zhang, Qiyu4; Zhu, Guanbao1
发表日期	2021-12-14
发表期刊	FRONTIERS IN ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	gastric cancer MAGEA3 immune infiltrates lymph node metastasis (LNM) immunotherapy
其他关键词	CELL LUNG-CANCER ; GASTROESOPHAGEAL JUNCTION ; CARCINOEMBRYONIC ANTIGEN ; PHASE-II ; MAGE-A3 ; MELANOMA ; PROTEIN ; CHEMOTHERAPY ; ADJUVANT ; IMMUNOTHERAPY
摘要	Gastric cancer (GC) is an aggressive malignant tumor and causes a significant number of deaths every year. With the coming of the age of cancer immunotherapy, search for a new target in gastric cancer may benefit more advanced patients. Melanoma-associated antigen-A3 (MAGEA3), one of the members of the cancer-testis antigen (CTA) family, was considered an important part of cancer immunotherapy. We evaluate the potential role of MAGEA3 in GC through the TCGA database. The result revealed that MAGEA3 is upregulated in GC and linked to poor OS and lymph node metastasis. MAGEA3 was also correlated with immune checkpoints, TMB, and affected the tumor immune microenvironment and the prognosis of GC through CIBERSORT, TIMER, and Kaplan-Meier plotter database analysis. In addition, GSEA-identified MAGEA3 is involved in the immune regulation of GC. Moreover, the protein-protein interaction (PPI) networks of MAGEA3 were constructed through STRING database and MAGEA3-correlated miRNAs were screened based on the joint analysis of multiple databases. In terms of experimental verification, we constructed pET21a (+)/MAGEA3 restructuring plasmids and transformed to Escherichia coli Rosetta. MAGEA3 protein was used as an antigen after being expressed and purified and can effectively detect the specific IgG in 93 GC patients' serum specimens with 44.08% sensitivity and 92.54% specificity. Through further analysis, the positive rate of MAGEA3 was related to the stage and transfer number of lymph nodes. These results indicated that MAGEA3 is a novel biomarker and correlated with lymph node metastasis and immune infiltrates in GC, which could be a new target for immunotherapy.
资助项目	Public Welfare Foundation of Zhejiang Province [Lgf18h160030]; Wenzhou Basic Scientific Research Projects [Y20210941]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	2234-943X
卷号	11 页码:784925
DOI	10.3389/fonc.2021.784925
页数	15
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000742889700001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34970496
PMC记录号	PMC8712941
SCOPUSEID	2-s2.0-85121870746
通讯作者地址	[Zhu, Guanbao]Department of Gastrointestinal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China ; [Zhang, Qiyu]Department of Hepato-Bilio-Pancreatic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/19083
专题	附属第一医院附属第一医院_风湿免疫科基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Zhang, Qiyu; Zhu, Guanbao
作者单位	1.Department of Gastrointestinal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Rheumatology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Department of Medical Microbiology and Immunology,Wenzhou Medical University,Wenzhou,China; 4.Department of Hepato-Bilio-Pancreatic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Jin, Jinji,Tu, Jianxin,Ren, Jiahuan,et al. Comprehensive Analysis to Identify MAGEA3 Expression Correlated With Immune Infiltrates and Lymph Node Metastasis in Gastric Cancer[J]. FRONTIERS IN ONCOLOGY,2021,11:784925.
APA	Jin, Jinji., Tu, Jianxin., Ren, Jiahuan., Cai, Yiqi., Chen, Wenjing., ... & Zhu, Guanbao. (2021). Comprehensive Analysis to Identify MAGEA3 Expression Correlated With Immune Infiltrates and Lymph Node Metastasis in Gastric Cancer. FRONTIERS IN ONCOLOGY, 11, 784925.
MLA	Jin, Jinji,et al."Comprehensive Analysis to Identify MAGEA3 Expression Correlated With Immune Infiltrates and Lymph Node Metastasis in Gastric Cancer".FRONTIERS IN ONCOLOGY 11(2021):784925.