K-Ras promotes the non-small lung cancer cells survival by cooperating with sirtuin 1 and p27 under ROS stimulation

doi:10.1007/s13277-015-3429-8

科研成果详情

题名	K-Ras promotes the non-small lung cancer cells survival by cooperating with sirtuin 1 and p27 under ROS stimulation
作者	Cheng, Dezhi1,2; Zhao, Liang 1,3,4; Xu, Yunsheng1,5; Ou, Rongying1,6; Li, Gang7; Yang, Han7; Li, Wenfeng1,7
发表日期	2015-09
发表期刊	TUMOR BIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	K-Ras Sirtuin 1 p27 ROS stimulation Non-small lung cancer
其他关键词	GROWTH ARREST ; LIFE-SPAN ; SENESCENCE ; INHIBITOR ; PROTEIN ; SIR2 ; OVEREXPRESSION ; DEACETYLATION ; GENERATION ; ONCOGENES
摘要	Cigarette smoking might lead to lung cancer. However, the related signaling pathways at molecular level remained unknown until now. In this study, we studied the signaling processes associated between tobacco exposure and lung cancer. First, we detected and validated pathway-specific gene expression at bronchial epithelium. These proteins reflected the activation of signaling pathways relevant to tobacco exposure, including ATM, BCL2, GPX1, K-Ras, IKBKB, and SIRT1. Tobacco smoking was simulated via reactive oxygen species (ROS) pathway. ROS not only arrested cell cycle at G1/S stage but also increased expressions of Sirt1 and p27. Further studies showed that the expression of p27 was dependent on ERK1/2 activation, and p27 itself could halt cell cycle by inhibiting the activation of CDKs. Moreover, activation of K-Ras, the key regulator of Ras/ERK pathway, was tightly regulated by enzyme activity of Sirt1. Deacetylation of K-Ras by Sirt1 increased the transformation of Ras-GTP to Ras-GDP, promoting the activation of downstream of ERK1/2. In reverse, Ras/ERK pathway could also regulate Sirt1 transcription. In conclusion, inhibition of Sirt1 may be an effective strategy for the prevention of tumor progression in high-risk patients or as a therapeutic strategy in established tumors.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81373075, 81371748]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY14H16042, Y12H310019]
出版者	SAGE PUBLICATIONS LTD
出版地	LONDON
ISSN	1010-4283
EISSN	1423-0380
卷号	36 期号:9 页码:7221-7232
DOI	10.1007/s13277-015-3429-8
页数	12
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000365033100073
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25894374
SCOPUSEID	2-s2.0-84947126564
通讯作者地址	[Li, Wenfeng]Laboratory for Interdisciplinary Research,Institution for Translational Medicine,The First Affiliated Hospital of Wenzhou Medical University,No. 2 of Fuxue Lane,Wenzhou City,325000,China
Scopus学科分类	Medicine (all)
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/19044
专题	附属第一医院_心血管内科第一临床医学院（信息与工程学院）、附属第一医院_妇产科学
通讯作者	Li, Wenfeng
作者单位	1.Laboratory for Interdisciplinary Research,Institution for Translational Medicine,The First Affiliated Hospital of Wenzhou Medical University,No. 2 of Fuxue Lane,Wenzhou City,325000,China; 2.Department of Thoracic Cardiovascular,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.PET/CT Division of Imaging Center,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 4.Institution for Digital and Molecular Imaging,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 5.Department of Dermatovenerology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 6.Gynaecology and Obstetrics,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 7.Department of Chemoradiotherapy,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Cheng, Dezhi,Zhao, Liang,Xu, Yunsheng,et al. K-Ras promotes the non-small lung cancer cells survival by cooperating with sirtuin 1 and p27 under ROS stimulation[J]. TUMOR BIOLOGY,2015,36(9):7221-7232.
APA	Cheng, Dezhi., Zhao, Liang., Xu, Yunsheng., Ou, Rongying., Li, Gang., ... & Li, Wenfeng. (2015). K-Ras promotes the non-small lung cancer cells survival by cooperating with sirtuin 1 and p27 under ROS stimulation. TUMOR BIOLOGY, 36(9), 7221-7232.
MLA	Cheng, Dezhi,et al."K-Ras promotes the non-small lung cancer cells survival by cooperating with sirtuin 1 and p27 under ROS stimulation".TUMOR BIOLOGY 36.9(2015):7221-7232.