AMPK/PGC-1 alpha/GLUT4-Mediated Effect of Icariin on Hyperlipidemia-Induced Non-Alcoholic Fatty Liver Disease and Lipid Metabolism Disorder in Mice

doi:10.1134/S0006297921110055

科研成果详情

题名	AMPK/PGC-1 alpha/GLUT4-Mediated Effect of Icariin on Hyperlipidemia-Induced Non-Alcoholic Fatty Liver Disease and Lipid Metabolism Disorder in Mice
作者	Lin, Wei 1; Jin, Yin2; Hu, Xiang3; Huang, Erjiong2; Zhu, Qihan3
发表日期	2021-11
发表期刊	BIOCHEMISTRY-MOSCOW 影响因子和分区
语种	英语
原始文献类型	Article
关键词	icariin NAFLD model AMPK PGC-1 alpha GLUT4 pathway
其他关键词	ACETYL-COA CARBOXYLASE ; GLUCOSE-TRANSPORT ; SKELETAL-MUSCLE ; PPAR-ALPHA ; PGC-1-ALPHA ; AMPK ; EXPRESSION ; INHIBITION ; ACTIVATION ; CELLS
摘要	Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease in the world. Therapeutic activity of icariin, a major bioactive component of Epimedii Herba, in NAFLD is still unknown. Herein, the C57BL/6J mice were fed with a high-fat diet for 16 weeks to establish a NAFLD model. Mice were assigned to five groups: control group, NAFLD group, and icariin treatment groups. Effects of icariin on blood indices, glucose tolerance, insulin sensitivity, histopathological morphology, cell apoptosis, lipid accumulation, and AMPK signaling were analyzed. In addition, another cohort of mice were assigned to five groups: control group, NAFLD group, dorsomorphin treatment group, icariin treatment group, and dorsomorphin + icariin treatment group. Expression of proteins in liver tissues associated with AMPK signaling, and levels of ALT and AST were evaluated. Icariin attenuated the NAFLD-induced increase of the TG, TC, LDL-C, ALT, AST levels. HDL-C levels were affected neither by NAFLD nor by icariin. Furthermore, icariin treatment (100-200 mg/kg) counteracted the NAFLD-reduced glucose tolerance and insulin sensitivity and modulated histopathological changes, cell apoptosis, and lipid accumulation in liver tissues. Additionally, icariin mitigated the NAFLD-induced up-regulation of the cleaved caspase 3/9, SREBP-1c, and DGAT-2 levels, and enhanced the expression level of CPT-1, p-ACC/ACC, AMPK alpha 1, PGC-1 alpha, and GLUT4. Effects of icariin on the AMPK signaling and levels of AST and ALT could be reversed by AMPK inhibitor, dorsomorphin. This paper investigates the glucose-reducing and lipid-lowering effects of icariin in NAFLD. Moreover, icariin might function through activating the AMPK alpha 1/PGC-1 alpha/GLTU4 pathway.
资助项目	Wenzhou Municipal Sci-Tech Bureau Program [Y20190127]
出版者	MAIK NAUKA/INTERPERIODICA/SPRINGER
出版地	NEW YORK
ISSN	0006-2979
EISSN	1608-3040
卷号	86 期号:11 页码:1407-1417
DOI	10.1134/S0006297921110055
页数	11
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000717996600005
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34906049
SCOPUSEID	2-s2.0-85119081265
通讯作者地址	[Huang, Erjiong]Department of Gastroenterology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China ; [Zhu, Qihan]Department of Endocrinology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Biochemistry
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/18936
专题	附属第一医院_全科医学科附属第一医院_内分泌科附属第一医院_消化内科
通讯作者	Huang, Erjiong; Zhu, Qihan
作者单位	1.Department of General Medicine,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Gastroenterology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Endocrinology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Wei,Jin, Yin,Hu, Xiang,et al. AMPK/PGC-1 alpha/GLUT4-Mediated Effect of Icariin on Hyperlipidemia-Induced Non-Alcoholic Fatty Liver Disease and Lipid Metabolism Disorder in Mice[J]. BIOCHEMISTRY-MOSCOW,2021,86(11):1407-1417.
APA	Lin, Wei, Jin, Yin, Hu, Xiang, Huang, Erjiong, & Zhu, Qihan. (2021). AMPK/PGC-1 alpha/GLUT4-Mediated Effect of Icariin on Hyperlipidemia-Induced Non-Alcoholic Fatty Liver Disease and Lipid Metabolism Disorder in Mice. BIOCHEMISTRY-MOSCOW, 86(11), 1407-1417.
MLA	Lin, Wei,et al."AMPK/PGC-1 alpha/GLUT4-Mediated Effect of Icariin on Hyperlipidemia-Induced Non-Alcoholic Fatty Liver Disease and Lipid Metabolism Disorder in Mice".BIOCHEMISTRY-MOSCOW 86.11(2021):1407-1417.