Resistance and Heteroresistance to Colistin in Pseudomonas aeruginosa Isolates from Wenzhou, China

doi:10.1128/AAC.00556-19

科研成果详情

题名	Resistance and Heteroresistance to Colistin in Pseudomonas aeruginosa Isolates from Wenzhou, China
作者	Lin, Jie 1; Xu, Chunquan1; Fang, Renchi 1; Cao, Jianming2; Zhang, Xiucai 1; Zhao, Yajie 2; Dong, Guofeng 2; Sun, Yao 1; Zhou, Tieli1
发表日期	2019-10
发表期刊	ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Pseudomonas aeruginosa colistin heteroresistance lipid A modification molecular mechanisms resistance
其他关键词	2-COMPONENT REGULATORY SYSTEM ; POLYMYXIN-B ; ADAPTIVE RESISTANCE ; ACINETOBACTER ; MUTATIONS ; PMRAB ; TOLERANCE ; PEPTIDES ; FIELD
摘要	The goal was to investigate the mechanisms of colistin resistance and heteroresistance in Pseudomonas aeruginosa clinical isolates. Colistin resistance was determined by the broth microdilution method. Colistin heteroresistance was evaluated by population analysis profiling. Time-kill assays were also conducted. PCR sequencing was performed to detect the resistance genes among (hetero)resistant isolates, and quantitative real-time PCR assays were performed to determine their expression levels. Pulsed-field gel electrophoresis and multilocus sequence typing were performed. Lipid A characteristics were determined via matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS). Two resistant isolates and 9 heteroresistant isolates were selected in this study. Substitutions in PmrB were detected in 2 resistant isolates. Among heteroresistant isolates, 8 of 9 heteroresistant isolates had nonsynonymous PmrB substitutions, and 2 isolates, including 1 with a PmrB substitution, had PhoQ alterations. Correspondingly, the expression levels of pmrA or phoP were upregulated in PmrB- or PhoQ-substituted isolates. One isolate also found alterations in ParRS and CprRS. The transcript levels of the pmrH gene were observed to increase across all investigated isolates. MALDI-TOF MS showed additional 4-amino-4-deoxy-L-arabinose (L-Ara4N) moieties in lipid A profiles in (hetero)resistant isolates. In conclusion, both colistin resistance and heteroresistance in P. aeruginosa in this study mainly involved alterations of the PmrAB regulatory system. There were strong associations between mutations in specific genetic loci for lipid A synthesis and regulation of modifications to lipid A. The transition of colistin heteroresistance to resistance should be addressed in future clinical surveillance.
资助项目	Planned Science and Technology Project of Wenzhou [Y20170204]; Health Department of Zhejiang Province of the People's Republic of China [2018KY123]
出版者	AMER SOC MICROBIOLOGY
出版地	WASHINGTON
ISSN	0066-4804
EISSN	1098-6596
卷号	63 期号:10 页码:e00556-19.
DOI	10.1128/AAC.00556-19
页数	12
WOS类目	Microbiology ; Pharmacology & Pharmacy
WOS研究方向	Microbiology ; Pharmacology & Pharmacy
WOS记录号	WOS:000487320100033
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	31383654
PMC记录号	PMC6761505
SCOPUSEID	2-s2.0-85072558395
通讯作者地址	[Cao, Jianming]Department of Medical Lab Science,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou, Zhejiang Province,China
Scopus学科分类	Pharmacology;Pharmacology (medical);Infectious Diseases
TOP期刊	TOP期刊
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/18699
专题	第一临床医学院（信息与工程学院）、附属第一医院_临床检验诊断学检验医学院（生命科学学院、生物学实验教学中心）附属第一医院检验医学院（生命科学学院、生物学实验教学中心）_基础检验学系
通讯作者	Cao, Jianming
作者单位	1.Department of Clinical Laboratory,Wenzhou Medical University,Wenzhou, Zhejiang Province,China; 2.Department of Medical Lab Science,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou, Zhejiang Province,China
第一作者单位	温州医科大学
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）; 基础检验学系
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Lin, Jie,Xu, Chunquan,Fang, Renchi,et al. Resistance and Heteroresistance to Colistin in Pseudomonas aeruginosa Isolates from Wenzhou, China[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2019,63(10):e00556-19..
APA	Lin, Jie., Xu, Chunquan., Fang, Renchi., Cao, Jianming., Zhang, Xiucai., ... & Zhou, Tieli. (2019). Resistance and Heteroresistance to Colistin in Pseudomonas aeruginosa Isolates from Wenzhou, China. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 63(10), e00556-19..
MLA	Lin, Jie,et al."Resistance and Heteroresistance to Colistin in Pseudomonas aeruginosa Isolates from Wenzhou, China".ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 63.10(2019):e00556-19..