Human Adipose Tissue Lysate-Based Hydrogel for Lasting Immunomodulation to Effectively Improve Spinal Cord Injury Repair

doi:10.1002/smll.202304318

科研成果详情

题名	Human Adipose Tissue Lysate-Based Hydrogel for Lasting Immunomodulation to Effectively Improve Spinal Cord Injury Repair
作者	Wang, Yu 1,2,3,4,5; Chai, Ying-Qian1,2; Cai, Jie 6; Huang, Shan-Shan 1,2; Wang, Ye-Feng1,2,3; Yuan, Shan-Shan 1,2; Wang, Ji-Long 1,2,7; Shi, Ke-Qing1,2; Deng, Jun-Jie 1,2,7
发表日期	2023-11-28
发表期刊	Small (Weinheim an der Bergstrasse, Germany) 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Article ; Early Access ; Article in Press
关键词	human adipose tissue long-term anti-inflammation neural regeneration spinal cord injury tissue lysate-based hydrogel Degradation Macrophages Pathology Patient rehabilitation Proteins Tissue Tissue regeneration Anti-inflammation Anti-inflammatories Degradation rate Human adipose tissue Long-term anti-inflammation Lysates Neural regeneration Spinal cord injury Tissue lysate-based hydrogel
其他关键词	EXTRACELLULAR-MATRIX ; POLARIZATION ; REGENERATION ; MACROPHAGES ; EXPRESSION ; SCAFFOLDS ; BIOLOGY ; DRIVEN ; CELLS
摘要	The long-term inflammatory microenvironment is one of the main obstacles to inhibit acute spinal cord injury (SCI) repair. The natural adipose tissue-derived extracellular matrix hydrogel shows effective anti-inflammatory regulation because of its unique protein components. However, the rapid degradation rate and removal of functional proteins during the decellularization process impair the lasting anti-inflammation function of the adipose tissue-derived hydrogel. To address this problem, adipose tissue lysate provides an effective way for SCI repair due to its abundance of anti-inflammatory and nerve regeneration-related proteins. Thereby, human adipose tissue lysate-based hydrogel (HATLH) with an appropriate degradation rate is developed, which aims to in situ long-term recruit and induce anti-inflammatory M2 macrophages through sustainedly released proteins. HATLH can recruit and polarize M2 macrophages while inhibiting pro-inflammatory M1 macrophages regardless of human or mouse-originated. The axonal growth of neuronal cells also can be effectively improved by HATLH and HATLH-induced M2 macrophages. In vivo experiments reveal that HATLH promotes endogenous M2 macrophages infiltration in large numbers (3.5 × 105 /100 µL hydrogel) and maintains a long duration for over a month. In a mouse SCI model, HATLH significantly inhibits local inflammatory response, improves neuron and oligodendrocyte differentiation, enhances axonal growth and remyelination, as well as accelerates neurological function restoration
资助项目	National Natural Science Foundation of China[31971260];National Natural Science Foundation of China[32101127];Natural Science Foundation of Zhejiang Province[LY21H060008];Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholar[LR20C100001];Zhejiang Qianjiang Talent Project[QJD2002011];Zhejiang Basic Public Welfare Research Project[LGJ22C100001];Medical and Health Technology Project of Zhejiang Province[2024KY1263]
出版者	WILEY-V C H VERLAG GMBH
ISSN	1613-6810
EISSN	1613-6829
卷号	20 期号:16
DOI	10.1002/smll.202304318
页数	16
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS记录号	WOS:001109889300001
收录类别	PUBMED ; SCIE ; SCOPUS ; EI
EI入藏号	20234815127367
EI主题词	Hydrogels
EI分类号	461.1 Biomedical Engineering ; 461.2 Biological Materials and Tissue Engineering ; 461.5 Rehabilitation Engineering and Assistive Technology ; 461.6 Medicine and Pharmacology ; 461.9.1 Immunology ; 801.3 Colloid Chemistry ; 802.2 Chemical Reactions ; 804 Chemical Products Generally ; 804.1 Organic Compounds
URL	查看原文
PubMed ID	38018305
SCOPUSEID	2-s2.0-85177770569
通讯作者地址	[Shi, Ke-Qing]Joint Centre of Translational Medicine,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China ; [Deng, Jun-Jie]Joint Centre of Translational Medicine,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
Scopus学科分类	Biotechnology;Chemistry (all);Biomaterials;Materials Science (all);Engineering (miscellaneous)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/185829
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院其他_附属萧山医院（杭州市萧山区第一人民医院）
通讯作者	Shi, Ke-Qing; Deng, Jun-Jie
作者单位	1.Joint Centre of Translational Medicine,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 2.Joint Centre of Translational Medicine,Wenzhou Institute,University of Chinese Academy of Sciences,Zhejiang,Wenzhou,325000,China; 3.Department of Orthopedics (Spine Surgery),The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 4.Key Laboratory of Intelligent Treatment and Life Support for Critical Diseases of Zhejiang Province,Zhejiang,Wenzhou,325000,China; 5.Zhejiang Engineering Research Center for Hospital Emergency and Process Digitization,Zhejiang,Wenzhou,325000,China; 6.Department of Orthopedics,Xiaoshan Hospital Affiliated to Wenzhou Medical University,Zhejiang,Hangzhou,310000,China; 7.Zhejiang Engineering Research Center for Tissue Repair Materials,Wenzhou Institute,University of Chinese Academy of Sciences,Zhejiang,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Wang, Yu,Chai, Ying-Qian,Cai, Jie,et al. Human Adipose Tissue Lysate-Based Hydrogel for Lasting Immunomodulation to Effectively Improve Spinal Cord Injury Repair[J]. Small (Weinheim an der Bergstrasse, Germany),2023,20(16).
APA	Wang, Yu., Chai, Ying-Qian., Cai, Jie., Huang, Shan-Shan., Wang, Ye-Feng., ... & Deng, Jun-Jie. (2023). Human Adipose Tissue Lysate-Based Hydrogel for Lasting Immunomodulation to Effectively Improve Spinal Cord Injury Repair. Small (Weinheim an der Bergstrasse, Germany), 20(16).
MLA	Wang, Yu,et al."Human Adipose Tissue Lysate-Based Hydrogel for Lasting Immunomodulation to Effectively Improve Spinal Cord Injury Repair".Small (Weinheim an der Bergstrasse, Germany) 20.16(2023).