Universal and hypoimmunogenic pluripotent stem cells for clinical usage

doi:10.1016/bs.pmbts.2023.02.014

科研成果详情

题名	Universal and hypoimmunogenic pluripotent stem cells for clinical usage
作者	Sung, Tzu-Cheng 1; Maitiruze, Kailibinuer 1; Pan, Jiandong1; Gong, Jian2,3; Bai, Yongheng4; Pan, Xiaodong5; Higuchi, Akon1,6
发表日期	2023
发表期刊	Progress in molecular biology and translational science 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Research Support, Non-U.S. Gov't
关键词	Cell therapy Human leukocyte antigen Hypoimmunogenic hPS cells Immune privilege Off-the-shelf cells Universal hPS cells
其他关键词	CLASS-I ; HLA-G ; GENERATION ; EXPRESSION ; PLATELETS ; LINES ; REFRACTORINESS ; PROTECTION ; TOLERANCE ; LYSIS
摘要	It is urgent to prepare and store large numbers of clinical trial grade human pluripotent stem (hPS) cells for off-the-shelf use in stem cell therapies. However, stem cell banks, which store off-the-shelf stem cells, need financial support and large amounts of technicians for daily cell maintenance. Therefore, it is valuable to create "universal" or "hypoimmunogenic" hPS cells with genome editing engineering by knocking in or out immune-related genes. Only a small number of universal or hypoimmunogenic hPS cell lines should be needed to store for off-the-shelf usage and reduce the large amounts of instruments, consumables and technicians. In this article, we consider how to create hypoimmunogenic or universal hPS cells as well as the demerits of the technology. β2-Microglobulin-knockout hPS cells did not harbor human leukocyte antigen (HLA)-expressing class I cells but led to the activation of natural killer cells. To escape the activities of macrophages and natural killer cells, homozygous hPS cells having a single allele of an HLA class I gene, such as HLA-C, were proposed. Major HLA class Ia molecules were knocked out, and CD47, HLA-G and PD-L1 were knocked in hPS cells utilizing CRISPR/Cas9 genome editing. Finally, some researchers are trying to generate universal hPS cells without genome editing. The cells evaded the activation of not only T cells but also macrophages and natural killer cells. These universal hPS cells have high potential for application in cell therapy.
资助项目	LandSeed Hospital project [NCU-LSH-101-A-001, NCU-LSH-102-A-003]; Cathay General Hospital Medical Research Project [MR-A11029, MR-A11030]; National Defense Medical Center Project [111-NCU-TRI-01, 112-NCU-TRI-01, TSGH-A-111012, TSGH-A-112003]; Veteran Hospital Project [VGHUST111-G4-2-1, VGHUSTll2-G4-3-l]; Ministry of Science and Technology [110-2221-E-008-011, 111-2221-E-008-007, 111-2923-E-008-005-MY3, 112-2923-E-008-005-MY3]
出版者	ELSEVIER ACADEMIC PRESS INC
ISSN	1877-1173
EISSN	1878-0814
卷号	199 页码:271-296
DOI	10.1016/bs.pmbts.2023.02.014
页数	26
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS记录号	WOS:001103903200011
收录类别	PUBMED ; BKCI-S ; SCOPUS
URL	查看原文
PubMed ID	37678974
通讯作者地址	[Higuchi, Akon]Wenzhou Med Univ, Hosp Eye, State Key Lab Ophthalmol Optometry & Visual Sci, Wenzhou, Zhejiang, Peoples R China. ; [Higuchi, Akon]Natl Cent Univ, Dept Chem & Mat Engn, Taoyuan, Taiwan.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/185747
专题	眼视光学院（生物医学工程学院）、附属眼视光医院附属第一医院第二临床医学院，附属第二医院、育英儿童医院附属第二医院_检验科
通讯作者	Higuchi, Akon
作者单位	1.Wenzhou Med Univ, Hosp Eye, State Key Lab Ophthalmol Optometry & Visual Sci, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 2, Dept Lab Med, Wenzhou, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp Area 1, Affiliated Hosp 1, Key Lab Diag & Treatment Severe Hepatopancreat Di, Wenzhou, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp Area 1, Affiliated Hosp 1, Dept Urol, Wenzhou, Zhejiang, Peoples R China; 6.Natl Cent Univ, Dept Chem & Mat Engn, Taoyuan, Taiwan
第一作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
第一作者的第一单位	眼视光学院（生物医学工程学院）、附属眼视光医院
推荐引用方式 GB/T 7714	Sung, Tzu-Cheng,Maitiruze, Kailibinuer,Pan, Jiandong,et al. Universal and hypoimmunogenic pluripotent stem cells for clinical usage[J]. Progress in molecular biology and translational science,2023,199:271-296.
APA	Sung, Tzu-Cheng., Maitiruze, Kailibinuer., Pan, Jiandong., Gong, Jian., Bai, Yongheng., ... & Higuchi, Akon. (2023). Universal and hypoimmunogenic pluripotent stem cells for clinical usage. Progress in molecular biology and translational science, 199, 271-296.
MLA	Sung, Tzu-Cheng,et al."Universal and hypoimmunogenic pluripotent stem cells for clinical usage".Progress in molecular biology and translational science 199(2023):271-296.