Genomic and phylogenetic analysis of a multidrug-resistant mcr-1-carrying Klebsiella pneumoniae recovered from a urinary tract infection in China

doi:10.1016/j.jgar.2021.10.002

科研成果详情

名称	Genomic and phylogenetic analysis of a multidrug-resistant mcr-1-carrying Klebsiella pneumoniae recovered from a urinary tract infection in China
作者	Chen, Hangfei 1; Jiang, Tian2; Wu, Jianyong 3; Sun, Qingyang 4; Zha, Qiuying 1; Jin, Shurui 5; Ruan, Zhi 1
发表期刊	Journal of Global Antimicrobial Resistance;
语种	英语
原始文献类型	Note
关键词	Klebsiella pneumoniae mcr-1 Multidrug resistance Urinary tract infection Whole-genome sequencing
摘要	Objectives: The emergence and dissemination of colistin-resistant Enterobacterales has become a major global public-health threat. Here we investigated the genomic and phylogenetic characteristics of a multidrug-resistant Klebsiella pneumoniae strain (KP4823) carrying the mcr-1 gene recovered from a urinary tract infection in China. Methods: Antimicrobial susceptibility of K. pneumoniae KP4823 was determined by broth microdilution. Whole genomic DNA was extracted and sequenced using Oxford Nanopore MinION and Illumina NovaSeq 6000 platforms. Hybrid assembly with long and short reads was performed using Unicycler, and the genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The sequence type (ST), capsular type, and antimicrobial resistance and virulence genes were identified from the genome sequence. Core genome multilocus sequence typing (cgMLST) analysis was performed by BacWGSTdb 2.0 server. Results: Klebsiella pneumoniae KP4823 was resistant to colistin, ceftazidime, cefepime, cefotaxime, fosfomycin and aztreonam. The complete genome sequence of KP4823 consists of five contigs comprising 5 445 519 bp, including one chromosome and four plasmids. The isolate was assigned to ST101 with capsular serotype KL106. Several antimicrobial resistance genes were identified, including the colistin resistance gene mcr-1, which was located on a 34 685-bp IncX4 plasmid. The closest relative of K. pneumoniae KP4823 was another ST101 isolate (08EU827) recovered from Sweden in 2008, which differed by 191 cgMLST loci. Conclusion: Our study reports the genome sequence of a multidrug-resistant mcr-1-carrying K. pneumoniae in China. These data may help to understand the antimicrobial resistance mechanisms, genomic features and transmission dynamics of colistin resistance in clinical settings.
资助项目	Zhejiang Provincial Medical and Health Science and Technology Plan[2021KY197,2021KY943];
出版者	Elsevier Ltd
ISSN	2213-7165
EISSN	2213-7173
卷号	27
页码	222-224
DOI	10.1016/j.jgar.2021.10.002
收录类别	SCOPUS
发布日期	2021-12-01
URL	查看原文
PubMed ID	34653681
通讯作者地址	[Ruan, Zhi]Department of Clinical Laboratory,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,310016,China
Scopus学科分类	Microbiology;Immunology and Allergy;Immunology;Microbiology (medical)
SCOPUSEID	2-s2.0-85117732000
引用统计
文献类型	其他
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/185338
专题	温州医科大学
通讯作者	Ruan, Zhi
作者单位	1.Department of Clinical Laboratory,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,310016,China; 2.Department of Clinical Laboratory,The Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,317500,China; 3.Department of Clinical Laboratory,The Fourth Affiliated Hospital,Zhejiang University School of Medicine,Yiwu,322000,China; 4.Department of Clinical Laboratory,The 903rd Hospital of PLA,Hangzhou,310013,China; 5.Department of Mathematics,University of Waterloo,Waterloo,N2L 3G1,Canada
推荐引用方式 GB/T 7714	Chen, Hangfei,Jiang, Tian,Wu, Jianyong,et al. Genomic and phylogenetic analysis of a multidrug-resistant mcr-1-carrying Klebsiella pneumoniae recovered from a urinary tract infection in China. 2021.