Prediction of immunodominant peptide of HPV16 E5 protein and preparation of polyclonal antibody

科研成果详情

题名	Prediction of immunodominant peptide of HPV16 E5 protein and preparation of polyclonal antibody
作者	Tang, Wan-Lin; Wang, Lu-De; Gu, Mei-Ping; Saidu, Kamara; Wang, Qi; Li, Ming-Yang; Chen, Shao; Zhang, Li-Fang
发表日期	2021-06-01
发表期刊	Chinese Journal of Biologicals; 影响因子和分区
语种	英语
原始文献类型	Article
关键词	E5 protein Human papillomavirus ( HPV) Immunodominant peptide Polyclonal antibody
摘要	Objective To predict the immunodominant peptide of human papillomavirus (HPV) 16 E5 protein and prepare its polyclonal antibody. Methods Based on the full-lengdi amino acid sequence of human HPV 16 E5 protein, bioinformatics methods such as the Hopp & Woods hydrophilicity scheme, Zimmerman polarity parameter, Jameson-Wolf antigen index scheme and Emini surface accessibility scheme were combined with the secondary structure of E5 and its flexible region to comprehensively analyze the immunodominant fragment of E5. Japanese white rabbits were immunized with the E5 protein immunodominant peptide to verify the immunogenicity and prepare the polyclonal antibody. The titer and specificity of the prepared polyclonal antibody were determined by ELISA and indirect immunofluorescence assay (IFA) respectively. Results The amino acids 1-17 (MTNLDTASTTLLACFLL) at N-terminus was the immunodominant peptide of HPV 16 E5 protein, which induced high level of E5 protein-specific IgG antibody in rabbits. The antibody titer increased with the increasing doses. IFA showed that the prepared polyclonal antibody specifically recognized the E5 target protein expressed in the cytoplasm of TC-1-E5 cells. Conclusion The amino acid peptide (MTNLDTASTTLLACFLL) of HPV16 E5 protein showed good immunogenicity. The HPV16 E5 protein-specific polyclonal antibody was successfully prepared, which provided a basis for further study on the biological characteristics of HPV 16 E5 protein.
出版者	Changchun Institute of Biological Products
ISSN	1004-5503
卷号	34 期号:6 页码:725-729+739
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85131911711
通讯作者地址	[Zhang, Li-fang]Department of Microbiology & Immunology,Wenzhou Medical University,Zhejiang Province,Wenzhou,325035,China
Scopus学科分类	Biotechnology;Immunology;Biochemistry;Applied Microbiology and Biotechnology
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/185211
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Zhang, Li-Fang
作者单位	Department of Microbiology & Immunology,Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	病原生物学与免疫学系
通讯作者单位	病原生物学与免疫学系
第一作者的第一单位	病原生物学与免疫学系
推荐引用方式 GB/T 7714	Tang, Wan-Lin,Wang, Lu-De,Gu, Mei-Ping,et al. Prediction of immunodominant peptide of HPV16 E5 protein and preparation of polyclonal antibody[J]. Chinese Journal of Biologicals;,2021,34(6):725-729+739.
APA	Tang, Wan-Lin., Wang, Lu-De., Gu, Mei-Ping., Saidu, Kamara., Wang, Qi., ... & Zhang, Li-Fang. (2021). Prediction of immunodominant peptide of HPV16 E5 protein and preparation of polyclonal antibody. Chinese Journal of Biologicals;, 34(6), 725-729+739.
MLA	Tang, Wan-Lin,et al."Prediction of immunodominant peptide of HPV16 E5 protein and preparation of polyclonal antibody".Chinese Journal of Biologicals; 34.6(2021):725-729+739.