Role of STAT3/mTOR pathway in chronic kidney injury

科研成果详情

题名	Role of STAT3/mTOR pathway in chronic kidney injury
作者	Zheng, Shefeng; Liu, Jinnv; Zhao, Zhangjian; Song, Ruifang
发表日期	2020
发表期刊	American Journal of Translational Research; 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Apoptosis Autophagy Kidney injury Proliferation STAT3/mTOR
摘要	STAT3/mTOR pathway plays an important role in inflammation, cell growth, and proliferation. However, the role of STAT3/mTOR pathway in chronic kidney injury remains unclear. Folic acid was used to induce kidney injury C57BL/6 mouse model followed by analysis of serum creatinine, renal weight ratio changes, renal pathological changes and STAT3/mTOR pathway changes. Glomerular mesangial cells were divided into control group, model group, STAT3 inhibitor (S3I-201) group followed by analysis of cell proliferation by MTT assay, cell apoptosis by flow cytometry, formation of autophagosomes by electron microscopy, expression of STAT3/mTOR signaling proteins and autophagy proteins LC3II and p62 by Western blot, expression of E-cadherin and Vimentin by immunofluorescence. The serum creatinine and renal weight ratio was increased with obvious lesions and upregulated STAT3 and p-mTOR level. Compared with control group, the difference was statistically significant (P < 0.05). Folic acid-induced injury of mesangial cells showed inhibited cell proliferation, promoted apoptosis, increased LC3II expression, decreased p62 expression, increased autophagic vacuoles and expression of STAT3 and p-mTOR as well as decreased E-cadherin expression and increased Vimentin expression. The difference was statistically significant compared with control group (P < 0.05). All above changes were significantly reversed after treatment with STAT3 inhibitor S3I-201 (P < 0.05). Activated STAT3/mTOR pathway, enhanced autophagy, promoted apoptosis of mesangial cells and inhibited cell proliferation were found in mice with renal injury. Inhibition of STAT3/mTOR activation inhibits autophagy and cell apoptosis and promotes cell proliferation.
出版者	E-Century Publishing Corporation40 White Oaks LaneMadisonWI 53711
ISSN	1943-8141
卷号	12 期号:7 页码:3302-3310
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85089315418
通讯作者地址	[Song, Ruifang]Department of Nephrology,Third Affiliated Hospital of Wenzhou Medical University,No. 168 Ruifeng Road,Ruian, Zhejiang Province,China
Scopus学科分类	Molecular Medicine;Clinical Biochemistry;Cancer Research
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/185016
专题	其他_附属第三医院（瑞安市人民医院）
通讯作者	Song, Ruifang
作者单位	Department of Nephrology,Third Affiliated Hospital of Wenzhou Medical University,Ruian, Zhejiang Province,China
第一作者单位	其他_附属第三医院（瑞安市人民医院）
通讯作者单位	其他_附属第三医院（瑞安市人民医院）
第一作者的第一单位	其他_附属第三医院（瑞安市人民医院）
推荐引用方式 GB/T 7714	Zheng, Shefeng,Liu, Jinnv,Zhao, Zhangjian,et al. Role of STAT3/mTOR pathway in chronic kidney injury[J]. American Journal of Translational Research;,2020,12(7):3302-3310.
APA	Zheng, Shefeng, Liu, Jinnv, Zhao, Zhangjian, & Song, Ruifang. (2020). Role of STAT3/mTOR pathway in chronic kidney injury. American Journal of Translational Research;, 12(7), 3302-3310.
MLA	Zheng, Shefeng,et al."Role of STAT3/mTOR pathway in chronic kidney injury".American Journal of Translational Research; 12.7(2020):3302-3310.