Protein recombination of Curcuma wenyujin lectin and its effects on apoptosis, migration and invasion of HCT-116 cells 温郁金凝集素蛋白重组及对结肠癌 HCT-116 细胞凋亡、迁移和侵袭的影响

doi:10.7501/j.issn.1674-6376.2023.11.011

科研成果详情

题名	Protein recombination of Curcuma wenyujin lectin and its effects on apoptosis, migration and invasion of HCT-116 cells 温郁金凝集素蛋白重组及对结肠癌 HCT-116 细胞凋亡、迁移和侵袭的影响
作者	Zhou, Dan 1; Zhang, Liping 2; Feng, Mengyi 1; Zhu, Qiang 3; Ren, Xianying 4; Wu, Zhigang 1; Jiang, Chengxi 1
发表日期	2023
发表期刊	Drug Evaluation Research; 影响因子和分区
语种	中文
原始文献类型	Article
关键词	apoptosis Curcuma wenyujin lectin HCT-116 cells invasion migration mitochondrial apoptosis
摘要	Objective Curcuma wenyujin lectin (CWL) recombinant protein was obtained, and its effect on the apoptosis, migration and invasion of colon cancer cells were invesitgated. Methods According to the coding sequence of CWL gene, CWL was heterogeneously expressed and purified using Escherichia coli prokaryotic expression system. CCK-8 method was used to evaluate the toxicity of CWL to colon cancer HCT-116 cells, apoptosis was detected by flow cytometry, and cells were detected by scratch test. Migration ability, cell invasion ability was assessed using transwell chamber method. the expression levels of genes in signal pathways such as Caspase-3, Caspase-9, BAX and PARP were performed using real-time fluorescence quantitative PCR (qRT-PCR) analysis. Western blotting was used to detect the expression levels of cleaved-Casase-3, cleaved-Casase-9, BAX, and cleaved-PARP proteins. Results Recombinant purification yielded CWL of relative molecular weight of 3 × 104. High quality concentration of CWL caused to significant coagulation of red blood cells, and as the concentration of CWL decreases, its coagulation activity gradually decreases until it disappears. Compared with control group, CWL had a significant inhibitory effect on the activity of HCT-116 cells (P < 0.001), CWL of 40, 80 μg·mL-1 could significantly reduce the migration and invasion ability of HCT-116 cells (P < 0.001), and significantly increased the apoptosis rate of HCT-116 cells (P < 0.01, 0.001). CWL of 40, 80 μg·mL-1 significantly increased mRNA expression levels of Caspase-3, Caspase-9, BAX, and PARP (P < 0.05, 0.01, 0.001), while significantly increased protein expression levels of cleaved- Caspase-3, cleaved-Caspase-9, BAX, and cleaved-PAP (P < 0.05, 0.01). Conclusion CWL can promote HCT-116 cell apoptosis through the mitochondrial apoptosis pathway, inhibit HCT-116 cell migration and invasion, thereby exerting anti-tumor effects and providing a new source of drugs for the development of clinical tumor drugs.
ISSN	1674-6376
卷号	46 期号:11 页码:2366-2373
DOI	10.7501/j.issn.1674-6376.2023.11.011
收录类别	SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85177582506
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/184712
专题	药学院（分析测试中心）
通讯作者	Jiang, Chengxi
作者单位	1.School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China; 2.Institute of Life Sciences,Wenzhou University,Wenzhou,325035,China; 3.Anhui Jiren Pharmaceutical Co.,Ltd.,Bozhou,236800,China; 4.Wenzhou Ejin Pharmaceutical Co.,Ltd.,Yueqing,325600,China
第一作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Zhou, Dan,Zhang, Liping,Feng, Mengyi,等. Protein recombination of Curcuma wenyujin lectin and its effects on apoptosis, migration and invasion of HCT-116 cells 温郁金凝集素蛋白重组及对结肠癌 HCT-116 细胞凋亡、迁移和侵袭的影响[J]. Drug Evaluation Research;,2023,46(11):2366-2373.
APA	Zhou, Dan., Zhang, Liping., Feng, Mengyi., Zhu, Qiang., Ren, Xianying., ... & Jiang, Chengxi. (2023). Protein recombination of Curcuma wenyujin lectin and its effects on apoptosis, migration and invasion of HCT-116 cells 温郁金凝集素蛋白重组及对结肠癌 HCT-116 细胞凋亡、迁移和侵袭的影响. Drug Evaluation Research;, 46(11), 2366-2373.
MLA	Zhou, Dan,et al."Protein recombination of Curcuma wenyujin lectin and its effects on apoptosis, migration and invasion of HCT-116 cells 温郁金凝集素蛋白重组及对结肠癌 HCT-116 细胞凋亡、迁移和侵袭的影响".Drug Evaluation Research; 46.11(2023):2366-2373.