miRNA-660-3p inhibits malignancy in glioblastoma via negative regulation of APOC1-TGFβ2 signaling pathway

doi:10.1080/15384047.2023.2281459

科研成果详情

题名	miRNA-660-3p inhibits malignancy in glioblastoma via negative regulation of APOC1-TGFβ2 signaling pathway
作者	Yang, Zelin1,2; Yang, Liang1; Sun, Zhenkai1; Rong, Yuxi 1; Bai, Chenglian2; Dong, Qiaoxiang1,2; Jian, Lin 1
发表日期	2023
发表期刊	Cancer Biology and Therapy; 影响因子和分区
语种	英语
原始文献类型	Article
关键词	APOC1 Glioblastoma miRNA-660-3p TGFβ2 signaling pathway TGF beta 2 signaling pathway
其他关键词	APOLIPOPROTEIN C1 APOC1 ; PROGNOSTIC BIOMARKER ; PANCREATIC-CANCER ; LUNG-CANCER ; PROGRESSION ; BETA ; PROMOTES ; GROWTH ; CELLS ; PROLIFERATION
摘要	Glioblastoma as the most common and aggressive central nervous system tumor in adults. Its prognosis and therapeutic outcome are poor due to the limited understanding of its molecular mechanism. Apolipoprotein C-1 (APOC1) as a member of the apolipoprotein family that acts as a tumor promoter in various cancers. MicroRNA (miRNA) can silence gene expression and suppress tumor progression. However, the role of APOC1 and its upstream miRNA has not been explored in glioblastoma. Two glioblastoma cell lines (U87 and U251) were used to explore the role of APOC1 and its upstream miRNA-660-3p in glioblastoma tumorigenesis in vitro. Cells with APOC1/miRNA-660-3p overexpression or knockdown were assessed for their proliferation, migration, and invasion in vitro, and tumorigenesis in vivo. Gene and protein expression was assessed by qRT-PCR and western blot, respectively. Cell proliferation was assessed by the MTT assay and the EdU and Ki67 staining. Cell migration and invasion were assessed by the transwell assay. Tumorigenesis in vivo was assessed in U87 cells with a xenograft mouse model. APOC1 was overexpressed in glioblastoma compared with normal peritumoral tissue and was inversely related to patient prognosis. APOC1 overexpression promotes cell proliferation, migration, and invasion in vitro. APOC1 inhibition reduced tumor growth in vivo. miRNA-660-3p inhibits tumorigenesis by directly targeting APOC1. Mechanistically, APOC1 drives the malignancy of glioblastoma by activating the TGFβ2 signaling pathway. miRNA-660-3p suppresses tumorigenesis by targeting APOC1. Therefore, miRNA-660-3p/APOC1 axis can serve as potential intervention targets in managing glioblastoma progression.
资助项目	Wenzhou Municipal Sci-Tech Bureau[ZY2021007];Science and Technology Department of Zhejiang Province[GF22H162840];
出版者	TAYLOR & FRANCIS INC
ISSN	1538-4047
EISSN	1555-8576
卷号	24 期号:1
DOI	10.1080/15384047.2023.2281459
页数	11
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:001107671600001
收录类别	SCOPUS ; SCIE ; PUBMED
URL	查看原文
PubMed ID	37981873
SCOPUSEID	2-s2.0-85177447110
通讯作者地址	[Dong, Qiaoxiang]Wenzhou Medical University,Zhejiang,Wenzhou,China
Scopus学科分类	Molecular Medicine;Oncology;Pharmacology;Cancer Research
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/184704
专题	附属第二医院公共卫生学院
通讯作者	Dong, Qiaoxiang
作者单位	1.Department of Neurosurgery,The Second Affiliated Hospital and Yuying Children’s Hospital,Wenzhou Medical University,Wenzhou,China; 2.School of Public Health and Management,Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第二医院; 公共卫生学院
通讯作者单位	温州医科大学
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Yang, Zelin,Yang, Liang,Sun, Zhenkai,et al. miRNA-660-3p inhibits malignancy in glioblastoma via negative regulation of APOC1-TGFβ2 signaling pathway[J]. Cancer Biology and Therapy;,2023,24(1).
APA	Yang, Zelin., Yang, Liang., Sun, Zhenkai., Rong, Yuxi., Bai, Chenglian., ... & Jian, Lin. (2023). miRNA-660-3p inhibits malignancy in glioblastoma via negative regulation of APOC1-TGFβ2 signaling pathway. Cancer Biology and Therapy;, 24(1).
MLA	Yang, Zelin,et al."miRNA-660-3p inhibits malignancy in glioblastoma via negative regulation of APOC1-TGFβ2 signaling pathway".Cancer Biology and Therapy; 24.1(2023).