Tetrahydropalmatine: Orchestrating survival – Regulating autophagy and apoptosis via the PI3K/AKT/mTOR pathway in perforator flaps

doi:10.1016/j.biopha.2023.115887

科研成果详情

题名	Tetrahydropalmatine: Orchestrating survival – Regulating autophagy and apoptosis via the PI3K/AKT/mTOR pathway in perforator flaps
作者	Chen, Xuankuai1,2,3; Fu, Kejian1,2,3; Lai, Yingying1,2,3; Dong, Chengji1,2,3; Chen, Zhuliu1,2,3; Huang, Yingying 1,2,3; Li, Guangyao1,2,3; Jiang, Renhao1,2,3; Wu, Hongqiang1,2,3; Wang, Anyuan 1,2,3; Huang, Shaojie4; Shen, Liyan1,2,3; Gao, Weiyang1,2,3; Li, Shi 1,2,3
发表日期	2023-12-31
发表期刊	Biomedicine and Pharmacotherapy; 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Angiogenesis Apoptosis Autophagy Multi-territory perforator flap Oxidative stress Tetrahydropalmatine
其他关键词	INHIBITION ; CANCER ; TARGET ; INJURY ; DEATH
摘要	Background: Introduced in clinical practice in 1989, perforator flaps are vital for tissue defect repair, but they are challenged by distal necrosis. Tetrahydropalmatine (THP) from celandine is renowned for its anti-inflammatory and analgesic effects. This study investigates THP's use in perforator flaps. Methods: Thirty rats were divided into a control group and four THP concentration groups, while seventy-eight rats were categorized as control, THP, THP combined with rapamycin (RAP), and RAP alone. We created 11 cm by 2.5 cm multi-regional perforator flaps on rat backs, assessing survival blood flow and extracting skin flap tissue for autophagy, oxidative stress, apoptosis, and angiogenesis markers. Results: The THP group exhibited significantly reduced distal necrosis, increased blood flow density, and survival area on the seventh day compared to controls. Immunohistochemistry and Western blot results demonstrated improved anti-oxidative stress and angiogenesis markers, along with decreased autophagy and apoptosis indicators. Combining THP with RAP diminished flap survival compared to THP alone. This was supported by protein expression changes in the PI3K-AKT-mTOR pathway. Conclusion: THP enhances flap survival by modulating autophagy, reducing tissue edema, promoting angiogenesis, and mitigating apoptosis and oxidative stress. THP offers a potential strategy for enhancing multi-regional perforator flap survival through the PI3K/AKT/mTOR pathway. These findings highlight THP's promise in combatting perforator flap necrosis, uncovering a novel mechanism for its impact on flap survival.
资助项目	National Natural Science Foundation of China[81873942];Zhejiang Provincial Natural Science Foundation[LQ23H150001];Wenzhou Science＆Technology Bureau[Y20210182];Wenzhou Science and Technology Planning Project[ZY2021005];
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
ISSN	0753-3322
EISSN	1950-6007
卷号	169
DOI	10.1016/j.biopha.2023.115887
页数	12
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:001118490000001
收录类别	SCOPUS ; PUBMED ; SCIE
URL	查看原文
PubMed ID	37984303
SCOPUSEID	2-s2.0-85177164375
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/184694
专题	第二临床医学院，附属第二医院、育英儿童医院检验医学院（生命科学学院、生物学实验教学中心）附属第二医院
通讯作者	Li, Shi
作者单位	1.Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,325027,China; 2.Key Laboratory of Orthopaedics of Zhejiang Province,Wenzhou,325027,China; 3.The Second School of Medicine,Wenzhou Medical University,Wenzhou,325027,China; 4.Wenzhou Medical University School of Laboratory Medicine and Life Sciences,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Chen, Xuankuai,Fu, Kejian,Lai, Yingying,et al. Tetrahydropalmatine: Orchestrating survival – Regulating autophagy and apoptosis via the PI3K/AKT/mTOR pathway in perforator flaps[J]. Biomedicine and Pharmacotherapy;,2023,169.
APA	Chen, Xuankuai., Fu, Kejian., Lai, Yingying., Dong, Chengji., Chen, Zhuliu., ... & Li, Shi. (2023). Tetrahydropalmatine: Orchestrating survival – Regulating autophagy and apoptosis via the PI3K/AKT/mTOR pathway in perforator flaps. Biomedicine and Pharmacotherapy;, 169.
MLA	Chen, Xuankuai,et al."Tetrahydropalmatine: Orchestrating survival – Regulating autophagy and apoptosis via the PI3K/AKT/mTOR pathway in perforator flaps".Biomedicine and Pharmacotherapy; 169(2023).