Tissue and serum miR-149-3p/5p in hospitalized patients with inflammatory bowel disease: Correlation with disease severity and inflammatory markers

doi:10.1002/kjm2.12784

科研成果详情

题名	Tissue and serum miR-149-3p/5p in hospitalized patients with inflammatory bowel disease: Correlation with disease severity and inflammatory markers
作者	Luo, Shuang; Chen, Xi-Han
发表日期	2023-11-23
发表期刊	KAOHSIUNG JOURNAL OF MEDICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	biomarkers disease activity inflammatory bowel disease inflammatory cytokines microRNAs
其他关键词	ULCERATIVE-COLITIS ; CROHNS-DISEASE
摘要	This study aimed to investigate the expression levels of tissue and serum miR-149-3p and miR-149-5p in hospitalized patients with inflammatory bowel disease (IBD). A total of 35 ulcerative colitis (UC) patients, 12 Crohn's disease (CD) patients, and 25 healthy controls were included in the study. The miRNAs expressions were measured in tissue and serum samples using quantitative real-time polymerase chain reaction (qRT-PCR). Inflammatory biomarkers were measured, including serum albumin, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6), and fecal calprotectin. MiR-149-3p and miR-149-5p were significantly decreased in the inflamed areas of both CD and UC patients compared to tissue controls, which was consistent with decreased serum levels in IBD patients compared to healthy controls. When distinguishing UC patients from healthy controls, serum miR-149-3p showed 74% sensitivity and 96% specificity, while serum miR-149-5p exhibited 63% sensitivity and 96% specificity. In the CD versus healthy control comparison, miR-149-3p achieved 100% sensitivity and 96% specificity, while miR-149-5p demonstrated 92% sensitivity and 96% specificity. In the UC versus CD comparison, miR-149-5p showed 75% sensitivity and 77% specificity, while miR-149-3p displayed 67% sensitivity and 80% specificity. Significant correlations were identified between the tissue and serum expression of miR-149-3p/5p and disease activity scores, as well as inflammatory biomarkers in both CD and UC patients. Decreased expression of miR-149-3p and miR-149-5p is associated with disease activity in IBD patients. These miRNAs demonstrate diagnostic potential and may serve as biomarkers for monitoring disease activity in IBD.
出版者	WILEY
ISSN	1607-551X
EISSN	2410-8650
卷号	40 期号:2 页码:131-138
DOI	10.1002/kjm2.12784
页数	8
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:001109097200001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	37997516
SCOPUSEID	2-s2.0-85177560436
通讯作者地址	[Luo, Shuang]Department of Gastroenterology,Pingyang Hospital Affiliated to Wenzhou Medical University,Zhejiang,Wenzhou,325400,China
Scopus学科分类	Medicine (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/184063
专题	其他_附属平阳医院（平阳县人民医院）
通讯作者	Luo, Shuang
作者单位	Department of Gastroenterology,Pingyang Hospital Affiliated to Wenzhou Medical University,Zhejiang,China
第一作者单位	其他_附属平阳医院（平阳县人民医院）
通讯作者单位	其他_附属平阳医院（平阳县人民医院）
第一作者的第一单位	其他_附属平阳医院（平阳县人民医院）
推荐引用方式 GB/T 7714	Luo, Shuang,Chen, Xi-Han. Tissue and serum miR-149-3p/5p in hospitalized patients with inflammatory bowel disease: Correlation with disease severity and inflammatory markers[J]. KAOHSIUNG JOURNAL OF MEDICAL SCIENCES,2023,40(2):131-138.
APA	Luo, Shuang, & Chen, Xi-Han. (2023). Tissue and serum miR-149-3p/5p in hospitalized patients with inflammatory bowel disease: Correlation with disease severity and inflammatory markers. KAOHSIUNG JOURNAL OF MEDICAL SCIENCES, 40(2), 131-138.
MLA	Luo, Shuang,et al."Tissue and serum miR-149-3p/5p in hospitalized patients with inflammatory bowel disease: Correlation with disease severity and inflammatory markers".KAOHSIUNG JOURNAL OF MEDICAL SCIENCES 40.2(2023):131-138.