Nrf2 attenuates oxidative stress to mediate the protective effect of ciprofol against cerebral ischemia-reperfusion injury

doi:10.1007/s10142-023-01273-z

科研成果详情

题名	Nrf2 attenuates oxidative stress to mediate the protective effect of ciprofol against cerebral ischemia-reperfusion injury
作者	Liu, Xia1,2; Ren, Miao2; Zhang, Anqi2; Huang, Changshun 1; Wang, Junlu2
发表日期	2023-12
发表期刊	FUNCTIONAL & INTEGRATIVE GENOMICS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Ciprofol Cerebral ischemia-reperfusion injury Ferroptosis MCAO OGD/R Nrf2
其他关键词	CELL-DEATH ; FERROPTOSIS ; GPX4
摘要	Neuroinflammation and oxidative stress damage are involved in the pathogenesis of cerebral ischemia-reperfusion injury (CIRI). Ferroptosis emerged as a new player in the regulation of lipid peroxidation processes. This study aimed at exploring the potential involvement of ciprofol on ferroptosis-associated CIRI and subsequent neurological deficits in the mouse model of transient cerebral ischemia and reperfusion. Cerebral ischemia was built in male C57BL/6 J wild-type (WT) and Nrf2-knockout (Nrf2 KO) mice in the manner of middle cerebral artery occlusion (MCAO) followed by reperfusion. Ciprofol improved autonomic behavior, alleviated reactive oxygen species output and ferroptosis-induced neuronal death by nucleus transportation of NFE2 like BZIP transcription factor 2 (Nrf2) and the promotion of heme oxygenase 1 (Ho-1), solute carrier family 7 member 11 (SLC7A11/xCT), and glutathione peroxidase 4 (GPX4). Additionally, ciprofol improved neurological scores and reduced infarct volume, brain water content, and necrotic neurons. Cerebral blood flow in MCAO-treated mice was also improved. Furthermore, absence of Nrf2 abrogated the neuroprotective actions of ciprofol on antioxidant capacity and sensitized neurons to oxidative stress damage. In vitro, the primary-cultured cortical neurons from mice were pre-treated with oxygen-glucose deprivation/reperfusion (OGD/R), followed by ciprofol administration. Ciprofol effectively reversed OGD/R-induced ferroptosis and accelerated transcription of GPX4 and xCT. In conclusion, we investigated the ciprofol-induced inhibition effect of ferroptosis-sheltered neurons from lipid preoxidation in the pathogenesis of CIRI via Nrf2-xCT-GPX4 signaling pathway.
资助项目	Ningbo Health Science & Technology Plan Project Foundation
出版者	SPRINGER HEIDELBERG
ISSN	1438-793X
EISSN	1438-7948
卷号	23 期号:4
DOI	10.1007/s10142-023-01273-z
页数	18
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:001105702000002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	37996761
SCOPUSEID	2-s2.0-85177744386
通讯作者地址	[Huang, Changshun]Department of Anesthesiology,The First Affiliated Hospital of Ningbo University,Zhejiang Province,Ningbo,315000,China ; [Wang, Junlu]Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Province,Wenzhou,325000,China
Scopus学科分类	Genetics
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/184029
专题	第一临床医学院（信息与工程学院）、附属第一医院_麻醉科附属第一医院_麻醉科
通讯作者	Huang, Changshun; Wang, Junlu
作者单位	1.Department of Anesthesiology,The First Affiliated Hospital of Ningbo University,Zhejiang Province,Ningbo,315000,China; 2.Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Province,Wenzhou,325000,China
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院_麻醉科; 附属第一医院_麻醉科
通讯作者单位	第一临床医学院（信息与工程学院）、附属第一医院_麻醉科; 附属第一医院_麻醉科
推荐引用方式 GB/T 7714	Liu, Xia,Ren, Miao,Zhang, Anqi,et al. Nrf2 attenuates oxidative stress to mediate the protective effect of ciprofol against cerebral ischemia-reperfusion injury[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2023,23(4).
APA	Liu, Xia, Ren, Miao, Zhang, Anqi, Huang, Changshun, & Wang, Junlu. (2023). Nrf2 attenuates oxidative stress to mediate the protective effect of ciprofol against cerebral ischemia-reperfusion injury. FUNCTIONAL & INTEGRATIVE GENOMICS, 23(4).
MLA	Liu, Xia,et al."Nrf2 attenuates oxidative stress to mediate the protective effect of ciprofol against cerebral ischemia-reperfusion injury".FUNCTIONAL & INTEGRATIVE GENOMICS 23.4(2023).