| 题名 | Restoring Colistin Sensitivity and Combating Biofilm Formation: Synergistic Effects of Colistin and Usnic Acid against Colistin-Resistant Enterobacteriaceae |
| 作者 | |
| 发表日期 | 2023-11-09 |
| 发表期刊 | ACS infectious diseases 影响因子和分区 |
| 语种 | 英语 |
| 原始文献类型 | Journal Article ; Article |
| 关键词 | Enterobacteriaceae antibacterial antibiofilm colistin-resistance synergistic effect usnic acid |
| 其他关键词 | ESCHERICHIA-COLI ; MEMBRANE ; ANTIBACTERIAL ; PERSISTERS ; DISRUPTION ; MECHANISMS |
| 摘要 | Colistin (COL), the last line of defense in clinical medicine, is an important therapeutic option against multidrug-resistant Gram-negative bacteria. In this context, the emergence of colistin-resistant (COL-R) bacteria mediated by broad-spectrum efflux pumps, mobile genetic elements, and biofilm formation poses a significant public health concern. In response to this challenge, a novel approach of combining COL with usnic acid (UA) has been proposed in this study. UA is a secondary metabolite derived from lichens and is well-known for its anti-inflammatory properties. This study aimed to investigate the synergistic effects of UA and COL against COL-R Enterobacteriaceae both in vitro and in vivo. The exceptional synergistic antibacterial activity exhibited by the combination of COL and UA was demonstrated by performing a comprehensive set of assays, including the checkerboard assay, time-dependent killing assay, and Live/Dead bacterial cell viability assay. Furthermore, crystal violet staining and scanning electron microscopy assays revealed the inhibitory effect of this combination on the biofilm formation. Mechanistically, the combination of UA and COL exacerbated cell membrane rupture, induced DNA damage, and generated a significant amount of reactive oxygen species, which ultimately resulted in bacterial cell death. In addition, erythrocyte hemolysis and cell viability tests confirmed the biocompatibility of the combination. The evaluation of the COL/UA combination in vivo using Galleria mellonella larvae and a mouse infection model showed a significant improvement in the survival rate of the infected larvae as well as a reduction in the bacterial load in the mouse thigh muscle. These findings, for the first time, provide strong evidence for the potential application of COL/UA as an effective alternative therapeutic option to combat infections caused by COL-R Enterobacteriaceae strains |
| 出版者 | AMER CHEMICAL SOC |
| ISSN | 2373-8227 |
| 卷号 | 9期号:12页码:2457-2470 |
| DOI | 10.1021/acsinfecdis.3c00315 |
| 页数 | 14 |
| WOS类目 | Chemistry, Medicinal ; Infectious Diseases |
| WOS研究方向 | Pharmacology & Pharmacy ; Infectious Diseases |
| WOS记录号 | WOS:001121891400001 |
| 收录类别 | PUBMED ; SCOPUS ; SCIE |
| URL | 查看原文 |
| PubMed ID | 37944020 |
| SCOPUSEID | 2-s2.0-85178144388 |
| 通讯作者地址 | [Cao, Jianming]Department of Medical Lab Science,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Zhejiang Province,Wenzhou,32500,China ; [Zhou, Tieli]Department of Clinical Laboratory,The First Affiliated Hospital of Wenzhou Medical University;Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,Zhejiang Province,Wenzhou,325000,China |
| Scopus学科分类 | Infectious Diseases |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/183809 |
| 专题 | 附属第一医院 检验医学院（生命科学学院、生物学实验教学中心） |
| 通讯作者 | Cao, Jianming; Zhou, Tieli |
| 作者单位 | 1.Department of Clinical Laboratory,The First Affiliated Hospital of Wenzhou Medical University; Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,Zhejiang Province,Wenzhou,325000,China; 2.Department of Medical Lab Science,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Zhejiang Province,Wenzhou,32500,China |
| 第一作者单位 | 附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院; 检验医学院（生命科学学院、生物学实验教学中心）; 基础检验学系 |
| 通讯作者单位 | 检验医学院（生命科学学院、生物学实验教学中心）; 基础检验学系; 附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院 |
| 第一作者的第一单位 | 附属第一医院 |
| 推荐引用方式 GB/T 7714 | Zhang, Yi,Han, Yijia,Huang, Zeyu,et al. Restoring Colistin Sensitivity and Combating Biofilm Formation: Synergistic Effects of Colistin and Usnic Acid against Colistin-Resistant Enterobacteriaceae[J]. ACS infectious diseases,2023,9(12):2457-2470. |
| APA | Zhang, Yi., Han, Yijia., Huang, Zeyu., Huang, Yali., Kong, Jingchun., ... & Zhou, Tieli. (2023). Restoring Colistin Sensitivity and Combating Biofilm Formation: Synergistic Effects of Colistin and Usnic Acid against Colistin-Resistant Enterobacteriaceae. ACS infectious diseases, 9(12), 2457-2470. |
| MLA | Zhang, Yi,et al."Restoring Colistin Sensitivity and Combating Biofilm Formation: Synergistic Effects of Colistin and Usnic Acid against Colistin-Resistant Enterobacteriaceae".ACS infectious diseases 9.12(2023):2457-2470. |
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