Identification and Characterization of a Novel Retinoic Acid Response Element in Zebrafish cyp26a1 Promoter

doi:10.1002/ar.21520

科研成果详情

题名	Identification and Characterization of a Novel Retinoic Acid Response Element in Zebrafish cyp26a1 Promoter
作者	Li, Jingyun 1; Hu, Ping 1; Li, Kui 1; Zhao, Qingshun 1,2
发表日期	2012-02
发表期刊	ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	zebrafish cyp26a1 retinoic acid response element promoter transgenic zebrafish development
其他关键词	GENE-EXPRESSION ; METABOLIZING ENZYME ; POSTERIOR AXIS ; HINDBRAIN ; PATTERN ; EMBRYO ; CYTOCHROME-P450 ; INDUCIBILITY
摘要	Cyp26A1 is a major enzyme that controls retinoic acid (RA) homeostasis by metabolizing RA into bio-inactive metabolites. Previously, we demonstrated that zebrafish cyp26a1 promoter possesses two conserved RA response elements (RAREs; proximal R1 and distal R2) in response to RA. Here, we report that it contains a novel RARE (R3) lying between R1 and R2. Mutagenesis analysis reveals that R3 works together with R1 and R2 to ensure the maximum RA inducibility of cyp26a1 promoter. Performing electrophoretic mobility shift assay and chromatin immunoprecipitation assay, we show that RA receptor alpha can bind the novel RARE. Creating and analyzing transgenic zebrafish of Tg(cyp26a1-R3mut:eYFP)nju3/+ that harbor enhanced yellow fluorescent protein reporter gene (eYFP) driven by cyp26a1 promoter with mutated R3, we demonstrate that the reporter is mainly expressed in tissues of endogenous RA independent but not regions of RA dependent. Like Tg(cyp26a1:eYFP)nju1/+, which harbor eYFP driven by wild-type cyp26a1 promoter, the reporter in Tg(cyp26a1-R3mut:eYFP)nju3/+ responds to excessive RA dose dependently. However, it is expressed in a significantly lower level than the reporter in Tg(cyp26a1:eYFP)nju1/+ in response to exogenous RA. Taken together, our results demonstrate that zebrafish cyp26a1 promoter contains a novel RARE that plays crucial roles in regulating cyp26a1 expression during early development of zebrafish. Anat Rec, 2012. (C) 2011 Wiley Periodicals, Inc.
资助项目	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2007CB947101, 2011CB943804]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30871439]; Natural Science Foundation of Jiangsu ProvinceNatural Science Foundation of Jiangsu Province [BK2010390]
出版者	WILEY-BLACKWELL
出版地	MALDEN
ISSN	1932-8486
EISSN	1932-8486
卷号	295 期号:2 页码:268-277
DOI	10.1002/ar.21520
页数	10
WOS类目	Anatomy & Morphology
WOS研究方向	Anatomy & Morphology
WOS记录号	WOS:000298982700010
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	22190473
SCOPUSEID	2-s2.0-84855777876
通讯作者地址	[Zhao, Qingshun]Model Animal Research Center,Nanjing University,Pukou High-tech Development Zone,12 Xuefu Road,China
Scopus学科分类	Biotechnology;Anatomy;Ecology, Evolution, Behavior and Systematics;Histology
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/18366
专题	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Zhao, Qingshun
作者单位	1.Model Animal Research Center,MOE Key Laboratory of Model Animal for Disease Study,Nanjing University,China; 2.Zhejiang Provincial Key Lab for Technology and Application of Model Organisms,School of Life Sciences,Wenzhou Medical College,China
推荐引用方式 GB/T 7714	Li, Jingyun,Hu, Ping,Li, Kui,et al. Identification and Characterization of a Novel Retinoic Acid Response Element in Zebrafish cyp26a1 Promoter[J]. ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY,2012,295(2):268-277.
APA	Li, Jingyun, Hu, Ping, Li, Kui, & Zhao, Qingshun. (2012). Identification and Characterization of a Novel Retinoic Acid Response Element in Zebrafish cyp26a1 Promoter. ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, 295(2), 268-277.
MLA	Li, Jingyun,et al."Identification and Characterization of a Novel Retinoic Acid Response Element in Zebrafish cyp26a1 Promoter".ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY 295.2(2012):268-277.