Screening and identification of dominant B-cell epitopes of human cytomegalovirus UL138 and its clinical applications

doi:10.2217/fvl-2023-0052

科研成果详情

题名	Screening and identification of dominant B-cell epitopes of human cytomegalovirus UL138 and its clinical applications
作者	Zhu, Hua 1; Xu, Xuanlu1; Jiang, Jing 2; Xue, Xiangyang3; Zhang, Wenmiao1; Zou, Ruanmin1
发表日期	2023-08
发表期刊	FUTURE VIROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	B cell epitope ELISA full-length UL138 protein human cytomegalovirus
其他关键词	MEMORY
摘要	Aim: To predict the dominant B-cell epitope of the human cytomegalovirus UL138 gene and to provide a new assay for its latent infection. Methods: Recombinant plasmids were constructed with the predicted dominant epitopes, induced to be expressed and immunized in mice as a way to identify peptide antigenicity, immunogenicity and human serum. Results: Five potentially dominant B-cell epitopes were obtained. The positive rate of detection was found to be significantly higher in neonatal, adult and mouse serum specimens than in human cytomegalovirus (HCMV) (IgG, IgM) and UL138 gene DNA and cDNA assays (p < 0.05). Conclusion: The predicted dominant B-cell epitope is highly sensitive for the detection of human serum HCMV latent infection and can replace the traditional HCMV IgG assay.
资助项目	Wenzhou Public Welfare Science and Technology Project [Y20170013, Y20180019]; Center for Uterine Cancer Diagnosis & Therapy Research of Zhejiang Province
出版者	FUTURE MEDICINE LTD
ISSN	1746-0794
EISSN	1746-0808
卷号	18 期号:12 页码:783-795
DOI	10.2217/fvl-2023-0052
页数	13
WOS类目	Virology
WOS研究方向	Virology
WOS记录号	WOS:001077166900001
收录类别	SCIE ; SCOPUS
在线发表日期	2023-10
URL	查看原文
SCOPUSEID	2-s2.0-85176915200
通讯作者地址	[Zou, Ruanmin]Department of Obstetrics & Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
Scopus学科分类	Virology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/183591
专题	附属第一医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Zou, Ruanmin
作者单位	1.Department of Obstetrics & Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Department of Obstetrics & Gynecology,The People's Hospital of Yuhuan,Yuhuan,317600,China; 3.Department of Microbiology & Immunology,Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	附属第一医院; 妇产科学; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 妇产科学; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhu, Hua,Xu, Xuanlu,Jiang, Jing,et al. Screening and identification of dominant B-cell epitopes of human cytomegalovirus UL138 and its clinical applications[J]. FUTURE VIROLOGY,2023,18(12):783-795.
APA	Zhu, Hua, Xu, Xuanlu, Jiang, Jing, Xue, Xiangyang, Zhang, Wenmiao, & Zou, Ruanmin. (2023). Screening and identification of dominant B-cell epitopes of human cytomegalovirus UL138 and its clinical applications. FUTURE VIROLOGY, 18(12), 783-795.
MLA	Zhu, Hua,et al."Screening and identification of dominant B-cell epitopes of human cytomegalovirus UL138 and its clinical applications".FUTURE VIROLOGY 18.12(2023):783-795.