Aerobic Exercise Ameliorates Liver Injury in Db/Db Mice by Attenuating Oxidative Stress, Apoptosis and Inflammation Through the Nrf2 and JAK2/ STAT3 Signalling Pathways

doi:10.2147/JIR.S426581

科研成果详情

题名	Aerobic Exercise Ameliorates Liver Injury in Db/Db Mice by Attenuating Oxidative Stress, Apoptosis and Inflammation Through the Nrf2 and JAK2/ STAT3 Signalling Pathways
作者	Sun, Meiyan 1,2; Zhao, Xiaoyong 2; Li, Xingyue 2; Wang, Chunling 2; Lin, Lili3; Wang, Kaifang 2; Sun, Yingui 2; Ye, Wei 3; Li, Haiyan 3; Zhang, Ye 1; Huang, Chaolu 2,4
发表日期	2023
发表期刊	JOURNAL OF INFLAMMATION RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	db/db mice aerobic exercise inflammation apoptosis Nrf2 JAK2/STAT3
其他关键词	NONALCOHOLIC STEATOHEPATITIS ; DISEASE ; CELLS ; RATS
摘要	Objective: Diabetes mellitus (DM) implicates oxidative stress, apoptosis, and inflammation, all of which may contribute liver injury. Aerobic exercise is assured to positively regulate metabolism in the liver. This project was designed to investigate whether and how aerobic exercise improves DM-induced liver injury.Methods: Seven-week-old male db/db mice and age-matched m/m mice were randomly divided into a rest control group or a group that received 12 weeks of aerobic exercise by treadmill training (10 m/min). Haematoxylin and eosin (HE) staining, electron microscopy, Oil Red O staining and TUNEL assays were used to evaluate the histopathological changes in mouse liver. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TRIG), cholesterol (CHOL) were analyzed by serum biochemical analysis. Interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-alpha), and tissue levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were analyzed via ELISA. Nuclear factor E2-associated factor-2 (Nrf2), nuclear factor kappa B (NF-kappa B) and JAK2/STAT3 pathway-related proteins were measured by immunofluorescence, Western blotting and q-PCR. F4/80 expression in liver tissues was assessed by immunohistochemistry.Results: In diabetic mice, exercise training significantly decreased the levels of serum TRIG, CHOL, IL-6, TNF-alpha, ALT and AST; prevented weight gain, hyperglycaemia, and impaired glucose and insulin tolerance. Morphologically, exercise mitigated the diabetes induced increase in liver tissue microvesicles, inflammatory cells, F4/80 (macrophage marker) levels, and TUNEL-positive cells. In addition, exercise reduced the apoptosis index, which is consistent with the results for caspase-3 and Bax. Additionally, exercise significantly increased SOD activity, decreased MDA levels, activated Nrf2 and decreased the expression of NF-kB, phosphorylated JAK2 and STAT3 proteins in the livers of diabetic mice.Conclusion: This study demonstrated that aerobic exercise reversed liver dysfunction in db/db mice with T2DM by reducing oxidative stress, apoptosis and inflammation, possibly by enhancing Nrf2 expression and inhibiting the JAK2/STAT3 cascade response.
资助项目	Postgraduate Scientific Research Project of Higher Education Institutions of Anhui Province [YJS20210276]; Shandong Province Medical and Health Science and Technology Development Plan Project Fund [202104110334, 202104010549]
出版者	DOVE MEDICAL PRESS LTD
ISSN	1178-7031
EISSN	1178-7031
卷号	16 页码:4805-4819
DOI	10.2147/JIR.S426581
页数	15
WOS类目	Immunology
WOS研究方向	Immunology
WOS记录号	WOS:001089471000001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	37901382
SCOPUSEID	2-s2.0-85175153913
通讯作者地址	[Zhang, Ye]Department of Anesthesiology and Perioperative Medicine,The Second Affiliated Hospital of Anhui Medical University,Hefei,230601,China ; [Huang, Chaolu]Department of Anesthesiology,Affiliated Hospital of Weifang Medical University,Shandong Province,Weifang,261053,China
Scopus学科分类	Immunology and Allergy;Immunology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/183542
专题	第一临床医学院（信息与工程学院）、附属第一医院
通讯作者	Zhang, Ye; Huang, Chaolu
作者单位	1.Department of Anesthesiology and Perioperative Medicine,The Second Affiliated Hospital of Anhui Medical University,Hefei,230601,China; 2.Department of Anesthesiology,Affiliated Hospital of Weifang Medical University,Shandong Province,Weifang,261053,China; 3.The First Clinical Medical College,Wenzhou Medical University,Wenzhou,325000,China; 4.Department of Clinical Medicine,Qiandongnan Ethnic Vocational and Technical College,Kaili,556000,China
推荐引用方式 GB/T 7714	Sun, Meiyan,Zhao, Xiaoyong,Li, Xingyue,et al. Aerobic Exercise Ameliorates Liver Injury in Db/Db Mice by Attenuating Oxidative Stress, Apoptosis and Inflammation Through the Nrf2 and JAK2/ STAT3 Signalling Pathways[J]. JOURNAL OF INFLAMMATION RESEARCH,2023,16:4805-4819.
APA	Sun, Meiyan., Zhao, Xiaoyong., Li, Xingyue., Wang, Chunling., Lin, Lili., ... & Huang, Chaolu. (2023). Aerobic Exercise Ameliorates Liver Injury in Db/Db Mice by Attenuating Oxidative Stress, Apoptosis and Inflammation Through the Nrf2 and JAK2/ STAT3 Signalling Pathways. JOURNAL OF INFLAMMATION RESEARCH, 16, 4805-4819.
MLA	Sun, Meiyan,et al."Aerobic Exercise Ameliorates Liver Injury in Db/Db Mice by Attenuating Oxidative Stress, Apoptosis and Inflammation Through the Nrf2 and JAK2/ STAT3 Signalling Pathways".JOURNAL OF INFLAMMATION RESEARCH 16(2023):4805-4819.