Sildenafil Enhances the Therapeutic Effect of Islet Transplantation for Diabetic Peripheral Neuropathy via mTOR/S6K1 Pathway

doi:10.1155/2023/8199029

科研成果详情

题名	Sildenafil Enhances the Therapeutic Effect of Islet Transplantation for Diabetic Peripheral Neuropathy via mTOR/S6K1 Pathway
作者	Zhu, Xiandong 1; Xie, Shangjing2; Chen, Jiawei 2; Lu, Qiaohong2; Wang, Xiaowu2; Duan, Feixiang2; Xu, Sinian 3; Zhang, Yan 2; Huang, Hongjian 2; Wang, Yongqiang2; Wang, Hongwei 2; Chen, Bicheng2; Huang, Huanjie4
发表日期	2023-10-07
发表期刊	International journal of endocrinology 影响因子和分区
语种	英语
原始文献类型	Journal Article
其他关键词	NERVE GROWTH-FACTOR ; SCHWANN-CELLS ; PDE5 INHIBITORS ; HEALTH ; NGF
摘要	This study aimed to investigate the potential mechanism underlying the therapeutic effect of sildenafil in combination with islet transplantation for diabetic peripheral neuropathy., A streptozotocin-induced diabetic mouse model was established to evaluate the effects of islet transplantation and sildenafil intervention. The mice were subjected to different interventions for 6 weeks, and histopathological staining and immunohistochemistry techniques were employed to examine the pathological changes and protein expressions of BDNF, MBP, and cleaved caspase-3 in the sciatic nerve tissue. Moreover, RSC96 cells were cocultured with islet cells and sildenafil under high glucose conditions to investigate the potential involvement of the mTOR/S6K1 pathway, BDNF, and MBP proteins. Western blotting was used to detect protein expression in each group., The results showed that islet transplantation can restore sciatic nerve injury in diabetic mice, and sildenafil can enhance the therapeutic effect of islet transplantation. In addition, the combination of sildenafil and islet cells significantly upregulated the expression levels of mTOR/S6K1, BDNF, and MBP in RSC96 cells under high glucose conditions., Islet transplantation can reverse sciatic nerve injury in diabetic mice, and islet cells exhibit a protective effect on RSC96 cells under high glucose conditions via the activation of the mTOR/S6K1 pathway. Sildenafil enhances the therapeutic effect of islet transplantation, which may represent a potential treatment strategy for diabetic peripheral neuropathy.
资助项目	This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LY19H090014) and Wenzhou Science and Technology Bureau (Y20210132). [LY19H090014]; Zhejiang Provincial Natural Science Foundation of China [Y20210132]; Wenzhou Science and Technology Bureau
出版者	HINDAWI LTD
ISSN	1687-8337
EISSN	1687-8345
卷号	2023
DOI	10.1155/2023/8199029
页数	11
WOS类目	Endocrinology & Metabolism
WOS研究方向	Endocrinology & Metabolism
WOS记录号	WOS:001082200600001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	37841556
SCOPUSEID	2-s2.0-85175007642
通讯作者地址	[Chen, Bicheng]Key Laboratory of Diagnosis and Treatment,Severe Hepato-Pancreatic Diseases of Zhejiang Province,The First Affiliated Hospital,Wenzhou Medical University,Zhejiang Province,Wenzhou,325000,China
Scopus学科分类	Endocrinology, Diabetes and Metabolism;Endocrinology;Endocrine and Autonomic Systems
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/183414
专题	附属第一医院其他_定理临床学院（温州市中心医院）第一临床医学院（信息与工程学院）、附属第一医院_内科学_神经内科
通讯作者	Chen, Bicheng
作者单位	1.Department of Thyroid Surgery,The First Affiliated Hospital,Wenzhou Medical University,Zhejiang Province,Wenzhou,325000,China; 2.Key Laboratory of Diagnosis and Treatment,Severe Hepato-Pancreatic Diseases of Zhejiang Province,The First Affiliated Hospital,Wenzhou Medical University,Zhejiang Province,Wenzhou,325000,China; 3.Department of Neurosurgery,Wenzhou Central Hospital,Affiliated Dingli Clinical Institute,Wenzhou Medical University,Zhejiang Province,Wenzhou,325000,China; 4.Department of Neurology,The First Affiliated Hospital,Wenzhou Medical University,Zhejiang Province,Wenzhou,325000,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhu, Xiandong,Xie, Shangjing,Chen, Jiawei,et al. Sildenafil Enhances the Therapeutic Effect of Islet Transplantation for Diabetic Peripheral Neuropathy via mTOR/S6K1 Pathway[J]. International journal of endocrinology,2023,2023.
APA	Zhu, Xiandong., Xie, Shangjing., Chen, Jiawei., Lu, Qiaohong., Wang, Xiaowu., ... & Huang, Huanjie. (2023). Sildenafil Enhances the Therapeutic Effect of Islet Transplantation for Diabetic Peripheral Neuropathy via mTOR/S6K1 Pathway. International journal of endocrinology, 2023.
MLA	Zhu, Xiandong,et al."Sildenafil Enhances the Therapeutic Effect of Islet Transplantation for Diabetic Peripheral Neuropathy via mTOR/S6K1 Pathway".International journal of endocrinology 2023(2023).