Construction of a prognostic model for colorectal adenocarcinoma based on Zn transport-related genes identified by single-cell sequencing and weighted co-expression network analysis

doi:10.3389/fonc.2023.1207499

科研成果详情

题名	Construction of a prognostic model for colorectal adenocarcinoma based on Zn transport-related genes identified by single-cell sequencing and weighted co-expression network analysis
作者	Chen, Hua 1; Zhao, Ting2; Fan, Jianing 3; Yu, Zhiqiang4; Ge, Yiwen 3; Zhu, He 1; Dong, Pingping1; Zhang, Fu1; Zhang, Liang 4; Xue, Xiangyang2; Lin, Xiaoming1
发表日期	2023-09-26
发表期刊	Frontiers in oncology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Immune infiltration Zn transport colon adenocarcinoma (COAD) colorectal cancer (CRC) immune microenvironment immune microenvironment Colon adenocarcinoma (COAD) prognostic
其他关键词	ZINC UPTAKE TRANSPORTER ; BINDING PROTEIN P34 ; RIBOSOME-BINDING ; DNA-DAMAGE ; CANCER ; DEFICIENCY ; PROSTATE ; BREAST ; DYSREGULATION ; REPAIR
摘要	Colorectal cancer (CRC) is one of the most prevalent malignancies and the third most lethal cancer globally. The most reported histological subtype of CRC is colon adenocarcinoma (COAD). The zinc transport pathway is critically involved in various tumors, and its anti-tumor effect may be through improving immune function. However, the Zn transport pathway in COAD has not been reported., The determination of Zn transport-related genes in COAD was carried out through single-cell analysis of the GSE 161277 obtained from the GEO dataset. Subsequently, a weighted co-expression network analysis of the TCGA cohort was performed. Then, the prognostic model was conducted utilizing univariate Cox regression and least absolute shrinkage and selection operator (LASSO) Cox regression analysis. Functional enrichment, immune microenvironment, and survival analyses were also carried out. Consensus clustering analysis was utilized to verify the validity of the prognostic model and explore the immune microenvironment. Ultimately, cell experiments, including CCK-8,transwell and scratch assays, were performed to identify the function of LRRC59 in COAD., According to the Zn transport-related prognostic model, the individuals with COAD in TCGA and GEO databases were classified into high- and low-risk groups. The group with low risk had a comparatively more favorable prognosis. Two groups had significant variations in the immune infiltration, MHC, and the expression of genes related to the immune checkpoint. The cell experiments indicated that the proliferation, migration, and invasion of the HCT-116, DLD-1, and RKO cell lines were considerably increased after LRRC59 knockdown. It proved that LRRC59 was indeed a protective factor for COAD., A prognostic model for COAD was developed using zinc transport-related genes. This model can efficiently assess the immune microenvironment and prognosis of individuals with COAD. Subsequently, the function of LRRC59 in COAD was validated via cell experiments, highlighting its potential as a biomarker.
资助项目	This research was supported by Zhejiang Provincial Science and Technology Innovation Program (New Young Talent Program) for College Students (Grant No. 2023R413028). [2023R413028]; Zhejiang Provincial Science and Technology Innovation Program (New Young Talent Program) for College Students
出版者	FRONTIERS MEDIA SA
ISSN	2234-943X
卷号	13
DOI	10.3389/fonc.2023.1207499
页数	18
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:001078213400001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	37829346
SCOPUSEID	2-s2.0-85173693552
通讯作者地址	[Lin, Xiaoming]Department of Thoracic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China ; [Xue, Xiangyang]Department of Microbiology and Immunology,School of Basic Medical Science,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,Wenzhou Medical University,Zhejiang,Wenzhou,China ; [Zhang, Liang]Department of General Surgery,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
Scopus学科分类	Oncology;Cancer Research
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/183388
专题	附属第一医院温州医科大学第二临床医学院，附属第二医院、育英儿童医院基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Zhang, Liang; Xue, Xiangyang; Lin, Xiaoming
作者单位	1.Department of Thoracic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Department of Microbiology and Immunology,School of Basic Medical Science,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,Wenzhou Medical University,Zhejiang,Wenzhou,China; 3.School of Second Clinical Medical,Wenzhou Medical University,Zhejiang,Wenzhou,China; 4.Department of General Surgery,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院; 基础医学院（机能实验教学中心）_病原生物学与免疫学系; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Chen, Hua,Zhao, Ting,Fan, Jianing,et al. Construction of a prognostic model for colorectal adenocarcinoma based on Zn transport-related genes identified by single-cell sequencing and weighted co-expression network analysis[J]. Frontiers in oncology,2023,13.
APA	Chen, Hua., Zhao, Ting., Fan, Jianing., Yu, Zhiqiang., Ge, Yiwen., ... & Lin, Xiaoming. (2023). Construction of a prognostic model for colorectal adenocarcinoma based on Zn transport-related genes identified by single-cell sequencing and weighted co-expression network analysis. Frontiers in oncology, 13.
MLA	Chen, Hua,et al."Construction of a prognostic model for colorectal adenocarcinoma based on Zn transport-related genes identified by single-cell sequencing and weighted co-expression network analysis".Frontiers in oncology 13(2023).