Prednisolone Accelerates Embryonic Development of Zebrafish via Glucocorticoid Receptor Signaling at Low Concentrations

doi:10.1021/acs.est.3c02658

科研成果详情

题名	Prednisolone Accelerates Embryonic Development of Zebrafish via Glucocorticoid Receptor Signaling at Low Concentrations
作者	Wang, Congcong 1; Li, Meng 1; Gui, Wanying 1; Shi, Haochun 1; Wang, Peng 1; Chen, Jierong 1; Fent, Karl 2; Zhang, Kun 1; Dai, Jiayin 1; Li, Xi3; Zhao, Yanbin 1
发表日期	2023-10-09
发表期刊	Environmental science & technology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	acceleration embryonic development glucocorticoids glucocorticoids receptor signaling low concentration prednisolone zebrafish
其他关键词	DANIO-RERIO ; TREATMENT PLANTS ; SURFACE-WATER ; END-POINTS ; PHARMACEUTICALS ; ANDROGENS
摘要	Synthetic glucocorticoids have been widely detected in aquatic ecosystems and may pose a toxicological risk to fish. In the present study, we described multiple end point responses of zebrafish to a commonly prescribed glucocorticoid, prednisolone (PREL), at concentrations between 0.001 and 9.26 μg/L. Of 23 end points monitored, 7 were affected significantly. Significant increases in the frequency of yolk extension formation, spontaneous contraction, heart rate, and ocular melanin density and significant decreases of ear-eye distance at PREL concentrations of 0.001 μg/L and above clearly pointed to the acceleration of embryonic development of zebrafish by PREL. Further confirmation came from the alterations in somite numbers, head-trunk angle, and yolk sac size, as well as outcomes obtained via RNA sequencing, in which signaling pathways involved in tissue/organ growth and development were highly enriched in embryos upon PREL exposure. In addition, the crucial role of glucocorticoid receptor (GR) for PREL-induced effects was confirmed by both, the coexposure to antagonist mifepristone (RU486) and GR-/- mutant zebrafish experiments. We further demonstrated similar accelerations of embryonic development of zebrafish upon exposure to 11 additional glucocorticoids, indicating generic adverse effect characteristics. Overall, our results revealed developmental alterations of PREL in fish embryos at low concentrations and thus provided novel insights into the understanding of the potential environmental risks of glucocorticoids
资助项目	National Natural Science Foundation of China [22122605, 21976121, 42222708]; National Key Research and Development Program of China [2022YFC2705200]
出版者	AMER CHEMICAL SOC
ISSN	0013-936X
EISSN	1520-5851
卷号	57 期号:42 页码:15794-15805
DOI	10.1021/acs.est.3c02658
页数	12
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS研究方向	Engineering ; Environmental Sciences & Ecology
WOS记录号	WOS:001082681800001
收录类别	PUBMED ; SCIE ; SCOPUS ; EI
在线发表日期	2023-10
EI入藏号	20234515042324
EI主题词	Fish
EI分类号	454.3 Ecology and Ecosystems ; 461.2 Biological Materials and Tissue Engineering
URL	查看原文
PubMed ID	37812749
SCOPUSEID	2-s2.0-85175587206
通讯作者地址	[Zhao, Yanbin]State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants,School of Environmental Science and Engineering,Shanghai Jiao Tong University,800 Dongchuan Road,Shanghai,200240,China
Scopus学科分类	Chemistry (all);Environmental Chemistry
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/183358
专题	其他_附属康宁医院（温州市康宁医院）
通讯作者	Zhao, Yanbin
作者单位	1.State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants,School of Environmental Science and Engineering,Shanghai Jiao Tong University,800 Dongchuan Road,Shanghai,200240,China; 2.Institute of Biogeochemistry and Pollution Dynamics,Department of Environmental Systems Science,ETH Zürich,Zürich,CH-8092,Switzerland; 3.Center of Clinical Research,The Affiliated Kangning Hospital of Wenzhou Medical University,Wenzhou,325000,China
推荐引用方式 GB/T 7714	Wang, Congcong,Li, Meng,Gui, Wanying,et al. Prednisolone Accelerates Embryonic Development of Zebrafish via Glucocorticoid Receptor Signaling at Low Concentrations[J]. Environmental science & technology,2023,57(42):15794-15805.
APA	Wang, Congcong., Li, Meng., Gui, Wanying., Shi, Haochun., Wang, Peng., ... & Zhao, Yanbin. (2023). Prednisolone Accelerates Embryonic Development of Zebrafish via Glucocorticoid Receptor Signaling at Low Concentrations. Environmental science & technology, 57(42), 15794-15805.
MLA	Wang, Congcong,et al."Prednisolone Accelerates Embryonic Development of Zebrafish via Glucocorticoid Receptor Signaling at Low Concentrations".Environmental science & technology 57.42(2023):15794-15805.