Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis

doi:10.1186/s13018-023-04147-z

科研成果详情

题名	Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis
作者	Huang, Feiri 1,2; Su, Zhongliang 2; Yang, Jie 2; Zhao, Xizhen2; Xu, Yaozeng 1
发表日期	2023-09-11
发表期刊	Journal of orthopaedic surgery and research 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Apoptosis Cell cycle Inflammation NEAT1 Osteoarthritis PGAP1 miR374b-5p
其他关键词	PROMOTES CHONDROCYTE APOPTOSIS ; LONG NONCODING RNAS ; PGAP1 ; PROLIFERATION ; INFLAMMATION ; TARGETS
摘要	Osteoarthritis (OA), characterized by inflammation and articular cartilage degradation, is a prevalent arthritis among geriatric population. This paper was to scrutinize the novel mechanism of long noncoding RNA (lncRNA) NEAT1 in OA etiology., A total of 10 OA patients and 10 normal individuals was included in this study. Cell model of OA was built in human normal chondrocytes induced by lipopolysaccharide (LPS). An OA Wistar rat model was established through intra-articular injection of L-cysteine and papain mixtures (proportion at 1:2) into the right knee. Quantitative reverse transcription-polymerase chain reaction was employed to ascertain the expression levels of NEAT1, microRNA (miR)-374b-5p and post-GPI attachment to protein 1 (PGAP1), while dual-luciferase reporter experiments were used for the validation of target relationship among them. Cell cycle and apoptosis were calculated by flow cytometry analysis. CCK-8 assay was done to evaluate the proliferative potentials of chondrocytes. The levels of cell cycle-related proteins (Cyclin A1, Cyclin B1 and Cyclin D2) and pro-apoptotic proteins (Caspase3 and Caspase9) were measured by western blotting. Tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and IL-6 levels were determined via ELISA. Hematoxylin & eosin (HE) Staining was used for pathological examination in OA rats., Pronounced downregulation of NEAT1 and PGAP1 and high amounts of miR-374b-5p were identified in OA patients, LPS-induced chondrocytes and OA rats. NEAT1 targeted miR-374b-5p to control PGAP1 expression. Loss of NEAT1 or upregulation of miR-374b-5p dramatically accelerated apoptosis, led to the G1/S arrest and promoted the secretion of inflammatory cytokines in LPS-induced chondrocytes, while ectopic expression of PGAP1 exhibited the opposite influences on chondrocytes. Additionally, we further indicated that upregulation of miR-374b-5p attenuated the effects of PGAP1 overexpression on LPS-induced chondrocytes., Reduced NEAT1 induces the development of OA via miR-374b-5p/PGAP1 pathway. This suggests that the regulatory axis NEAT1/miR-374b-5p/PGAP1 is a novel and prospective target for OA treatment.
资助项目	We acknowledge the strong support of The Third Affiliated Hospital of Shanghai University, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University and The First Affiliated Hospital of Soochow University.; Third Affiliated Hospital of Shanghai University; Wenzhou Third Clinical Institute; First Affiliated Hospital of Soochow University
出版者	BMC
ISSN	1749-799X
卷号	18 期号:1
DOI	10.1186/s13018-023-04147-z
页数	16
WOS类目	Orthopedics
WOS研究方向	Orthopedics
WOS记录号	WOS:001070119500001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	37691099
SCOPUSEID	2-s2.0-85170395225
通讯作者地址	[Xu, Yaozeng]Department of Orthopedics,The First Affiliated Hospital of Soochow University,No.188 Shizi Street, Jiangsu,Suzhou,215000,China
Scopus学科分类	Surgery;Orthopedics and Sports Medicine
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/182852
专题	其他_温州市第三临床学院（温州市人民医院）
通讯作者	Xu, Yaozeng
作者单位	1.Department of Orthopedics,The First Affiliated Hospital of Soochow University,No.188 Shizi Street, Jiangsu,Suzhou,215000,China; 2.Department of Orthopedics,The Third Affiliated Hospital of Shanghai University,The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	其他_温州市第三临床学院（温州市人民医院）
推荐引用方式 GB/T 7714	Huang, Feiri,Su, Zhongliang,Yang, Jie,et al. Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis[J]. Journal of orthopaedic surgery and research,2023,18(1).
APA	Huang, Feiri, Su, Zhongliang, Yang, Jie, Zhao, Xizhen, & Xu, Yaozeng. (2023). Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis. Journal of orthopaedic surgery and research, 18(1).
MLA	Huang, Feiri,et al."Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis".Journal of orthopaedic surgery and research 18.1(2023).