Molecular characterization and comparison of bla NDM-1-carrying and bla NDM-5-harboring IncX3-type plasmids in carbapenem-resistant Klebsiella pneumoniae

doi:10.1128/spectrum.01028-23

科研成果详情

题名	Molecular characterization and comparison of bla NDM-1-carrying and bla NDM-5-harboring IncX3-type plasmids in carbapenem-resistant Klebsiella pneumoniae
作者	Yunxing Yang 1; Haiyang Liu 2; Lingxia Chen 2; Minjie Mao 3; Xiaofan Zhang 2; Longjie Zhou 2; Darong Duan 4; Xi Li 2; Hua Zhou 5
发表日期	2023-08-25
发表期刊	Microbiology spectrum 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	IncX3 NDM-1 NDM-5 carbapenemases fitness
摘要	Carbapenem-resistant Klebsiella pneumoniae (CRKP), which harbors the bla NDM plasmid, has been reported extensively and is considered a global threat clinically. However, characterization and comparisons of bla NDM-1-carrying and bla NDM-5-harboring IncX3-type plasmids in CRKP are lacking. Here, we systematically compared the differences in the characteristics, genetic backgrounds, transferability, and fitness costs between bla NDM-1-carrying and bla NDM-5-carrying plasmids in K. pneumoniae isolates. Fifteen NDM-producing CRKP isolates were recovered from 1376 CRKP isolates between 2019 and 2021, of which 4 were positive for bla NDM-1 and 11 were positive for bla NDM-5. All strains were highly resistant to carbapenem but remained susceptible to tigecycline and colistin. Core-genome-based phylogenetic analyses revealed that these strains were not clonally related. Whole-genome sequencing showed that bla NDM-1 and bla NDM-5 were located on ~54 kb and ~46 kb IncX3-type plasmids, respectively. The backbone, genetic context, and fitness cost of the bla NDM-1-bearing plasmid were highly similar to those of the bla NDM-5-carrying plasmid, but the transferability of the bla NDM-1-positive plasmid was greater than that of the bla NDM-5-positive plasmid. In conclusion, the transmission of bla NDM-1 or bla NDM-5 is mainly disseminated by plasmids rather than clonal spread. The high transfer frequency of the IncX3 plasmid facilitates the prevalence and dissemination of NDM-KP among Enterobacteriaceae. IMPORTANCE The emergence of NDM-producing Klebsiella pneumoniae is a severe challenge to public health. The widespread presence of bla NDM-1 and bla NDM-5 in Enterobacteriaceae has aroused broad concern. In this study, we performed molecular characterization of bla NDM-1-carrying and bla NDM-5-harboring IncX3-type plasmids in carbapenem-resistant Klebsiella pneumoniae (CRKP) and compared their phenotypes between strains with different bla NDM subtype. Our findings highlight the importance of IncX3-type plasmids in the transfer of the bla NDM-1 and bla NDM-5 genes and demonstrate that the bla NDM-1 plasmid possesses higher transfer ability. These data will provide important insights into carbapenem resistance gene transfer via plasmids and their further spread in clinical settings
资助项目	Medical and Health Technology Project of Hangzhou[A20210087];Zhejiang Provincial Public Projects[LGD21H190001];Research and Development Program of Zhejiang Province[2023C03068];National Natural Science Foundation of China[82172306];Medical and Health Research Project of Zhejiang Province[2022KY531];
ISSN	2165-0497
卷号	11 期号:5
DOI	10.1128/spectrum.01028-23
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	37623430
SCOPUSEID	2-s2.0-85175554337
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/182496
专题	其他_附属黄岩医院（台州市第一人民医院）
作者单位	1.Department of Clinical Laboratory, Affiliated Hangzhou First People's Hospital, Zhejiang School of Medicine , Hangzhou, China.; 2.Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College) , Hangzhou, Zhejiang, China.; 3.Department of Clinical Laboratory, The First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University , Jiaxing, Zhejiang, China.; 4.Department of Laboratory Medicine, Huangyan Hospital of Wenzhou Medical University, Taizhou First People' s Hospital , Taizhou, Zhejiang, China.; 5.Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine , Hangzhou, Zhejiang, China.
推荐引用方式 GB/T 7714	Yunxing Yang,Haiyang Liu,Lingxia Chen,et al. Molecular characterization and comparison of bla NDM-1-carrying and bla NDM-5-harboring IncX3-type plasmids in carbapenem-resistant Klebsiella pneumoniae[J]. Microbiology spectrum,2023,11(5).
APA	Yunxing Yang., Haiyang Liu., Lingxia Chen., Minjie Mao., Xiaofan Zhang., ... & Hua Zhou. (2023). Molecular characterization and comparison of bla NDM-1-carrying and bla NDM-5-harboring IncX3-type plasmids in carbapenem-resistant Klebsiella pneumoniae. Microbiology spectrum, 11(5).
MLA	Yunxing Yang,et al."Molecular characterization and comparison of bla NDM-1-carrying and bla NDM-5-harboring IncX3-type plasmids in carbapenem-resistant Klebsiella pneumoniae".Microbiology spectrum 11.5(2023).