microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment

doi:10.3892/or.2012.1690

科研成果详情

题名	microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment
作者	Wang, Chengde1; Su, Zhipeng1; Sanai, Nader 2; Xue, Xiangyang3; Lu, Lijun 4; Chen, Yunxiang 1; Wu, Jinsen1; Zheng, Weiming1; Zhuge, Qichuan1; Wu, Zhe Bao 1
发表日期	2012-05
发表期刊	ONCOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	microRNAs prolactinoma bromocriptine PDGFA microarray
其他关键词	BONE MORPHOGENETIC PROTEIN-4 ; PITUITARY-ADENOMAS ; DOWN-REGULATION ; D2 RECEPTORS ; CELLS ; DOPAMINE ; CANCER ; IDENTIFICATION ; INVOLVEMENT ; OCTREOTIDE
摘要	Little is known about the function of microRNAs in prolactinomas treated with bromocriptine. The aim of the study was to explore the microRNAs associated with bromocriptine-treated prolactinomas. Six prolactinoma samples were selected according to whether they received bromocriptine treatment or not before microsurgery, and microRNA expression profiles of bromocriptine-treated and untreated prolactinomas were screened by the miRCURY LNA Array. The differentially expressed microRNAs in microarrays were further validated by stem-loop real-time PCR and subjected to gene ontology analysis and KEGG pathway analysis. In addition, related genes of microRNAs were analyzed by qRT-PCR in 15 prolactinoma samples. The initial analysis by microarrays generated a list of 80 upregulated microRNAs and 71 down regulated microRNAs in treated prolactinomas compared to untreated prolactinomas. miR-206, miR-516b and miR-550 were confirmed to be significantly upregulated, while miR-671-5p was confirmed to be significantly downregulated in treated prolactinomas by qRT-PCR. microRNA-mRNA network analysis integrating GO and KEGG pathway annotation displayed some critical factors. Platelet-derived growth factor a polypeptide (PDGFA) and bone morphogenetic protein 4 (BMP4), were verified to be differentially expressed between the two groups. PDGFA was significantly upregulated in treated prolactinomas, while BMP4 was significantly downregulated in treated prolactinomas. Our study reveals differential expression of microRNAs in prolactinoma after pharmacotherapy. Specific microRNAs may be involved in the inhibition or promotion of prolactinoma tumor growth impacted by bromocriptine pharmacotherapy. PDGFA and BMP4 may be involved in the pharmacotherapy mechanism of prolactinoma.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [R2091137]; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1021-335X
EISSN	1791-2431
卷号	27 期号:5 页码:1312-1320
DOI	10.3892/or.2012.1690
页数	9
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000302202600002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	22366961
SCOPUSEID	2-s2.0-84863260561
通讯作者地址	[Wu, Zhe Bao]Wenzhou Med Coll, Dept Neurosurg, Affiliated Hosp 1, Wenzhou 325000, Peoples R China.
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/18247
专题	附属第一医院_神经外科附属第一医院_外科实验室检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
通讯作者	Wu, Zhe Bao
作者单位	1.Wenzhou Med Coll, Dept Neurosurg, Affiliated Hosp 1, Wenzhou 325000, Peoples R China; 2.Barrow Neurol Inst, Barrow Brain Tumor Res Ctr, Phoenix, AZ 85013 USA; 3.Wenzhou Med Coll, Dept Microbiol & Immunol, Wenzhou 325000, Peoples R China; 4.Wenzhou Med Coll, Key Lab Surg, Affiliated Hosp 1, Wenzhou 325000, Peoples R China
第一作者单位	附属第一医院; 神经外科
通讯作者单位	附属第一医院; 神经外科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Wang, Chengde,Su, Zhipeng,Sanai, Nader,et al. microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment[J]. ONCOLOGY REPORTS,2012,27(5):1312-1320.
APA	Wang, Chengde., Su, Zhipeng., Sanai, Nader., Xue, Xiangyang., Lu, Lijun., ... & Wu, Zhe Bao. (2012). microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment. ONCOLOGY REPORTS, 27(5), 1312-1320.
MLA	Wang, Chengde,et al."microRNA expression profile and differentially-expressed genes in prolactinomas following bromocriptine treatment".ONCOLOGY REPORTS 27.5(2012):1312-1320.