Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and blaNDM-1 co-harboring multidrug-resistant Enterobacter asburiae

doi:10.1016/j.csbj.2023.08.004

科研成果详情

题名	Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and blaNDM-1 co-harboring multidrug-resistant Enterobacter asburiae
作者	Li, Xinyang 1; Jiang, Tian1,2; Wu, Chenghao 1; Kong, Yingying 1,3; Ma, Yilei 1,3; Wu, Jianyong 4; Xie, Xinyou 1,3; Zhang, Jun 1,3; Ruan, Zhi 1,3
发表日期	2023
发表期刊	Computational and structural biotechnology journal 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Colistin resistance Enterobacter asburiae Lipid A blaNDM-1 mcr-10
其他关键词	COLISTIN RESISTANCE ; CLOACAE ; GENE
摘要	Colistin is considered as one of the last-resort antimicrobial agents for treating multidrug-resistant bacterial infections. Multidrug-resistant E. asburiae has been increasingly isolated from clinical patients, which posed a great challenge for antibacterial treatment. This study aimed to report a mcr-10 and blaNDM-1 co-carrying E. asburiae clinical isolate 5549 conferred a high-level resistance against colistin. Antibiotic susceptibility testing was performed using the microdilution broth method. Transferability of mcr-10 and blaNDM-1-carrying plasmids were investigated by conjugation experiments. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify modifications in lipid A. Whole genome sequencing and phylogenetic analysis between strain 5549 and a total of 301 E. asburiae genomes retrieved from NCBI database were performed. The genetic characteristics of mcr-10 and blaNDM-1-bearing plasmids were also analyzed. Our study indicated that strain 5549 showed extensively antibiotic-resistant trait, including colistin and carbapenem resistance. The mcr-10 and blaNDM-1 were carried by IncFIB/IncFII type p5549_mcr-10 (159417 bp) and IncN type p5549_NDM-1 (63489 bp), respectively. Conjugation assays identified that only the blaNDM-1-carrying plasmid could be successfully transferred to E. coli J53. Interestingly, mcr-10 did not mediate colistin resistance when it was cloned into E. coli DH5α. Mass spectrometry analysis showed the lipid A palmitoylation of the C-lacyl-oxo-acyl chain to the chemical structure of lipid A at m/z 2063 in strain 5549. In summary, this study is the first to report a mcr-10 and blaNDM-1 co-occurrence E. asburiae recovered from China. Our investigation revealed the distribution of different clonal lineage of E. asburiae with epidemiology perspective and the underlying mechanisms of colistin resistance. Active surveillance is necessary to control the further dissemination of multidrug-resistant E. asburiae
资助项目	National Natural Science Foundation of China [81871696, LR23H200001]; Zhejiang Provincial Natural Sci- ence Foundation of China [LY21H190001, 2020RC066]; Zhejiang Provincial Medical and Health Science and Technology Plan [2021RC007]; [82202565]
出版者	ELSEVIER
ISSN	2001-0370
EISSN	2001-0370
卷号	21 页码:3885-3893
DOI	10.1016/j.csbj.2023.08.004
页数	9
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:001058828900001
收录类别	PUBMED ; SCIE ; EI ; SCOPUS
在线发表日期	2023-08
EI入藏号	20233214509694
EI主题词	Antibiotics
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.8.1 Genetic Engineering ; 801 Chemistry ; 802.3 Chemical Operations
URL	查看原文
PubMed ID	37602227
SCOPUSEID	2-s2.0-85166963882
通讯作者地址	[Xie, Xinyou]Department of Clinical Laboratory,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,China
Scopus学科分类	Biotechnology;Biophysics;Structural Biology;Biochemistry;Genetics;Computer Science Applications
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/182469
专题	温州医科大学
通讯作者	Xie, Xinyou
作者单位	1.Department of Clinical Laboratory,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,China; 2.Department of Clinical Laboratory,The Affiliated Wenling Hospital,Wenzhou Medical University,Taizhou,China; 3.Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province,Hangzhou,China; 4.Department of Clinical Laboratory,The Fourth Affiliated Hospital,Zhejiang University School of Medicine,Yiwu,China
推荐引用方式 GB/T 7714	Li, Xinyang,Jiang, Tian,Wu, Chenghao,et al. Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and blaNDM-1 co-harboring multidrug-resistant Enterobacter asburiae[J]. Computational and structural biotechnology journal,2023,21:3885-3893.
APA	Li, Xinyang., Jiang, Tian., Wu, Chenghao., Kong, Yingying., Ma, Yilei., ... & Ruan, Zhi. (2023). Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and blaNDM-1 co-harboring multidrug-resistant Enterobacter asburiae. Computational and structural biotechnology journal, 21, 3885-3893.
MLA	Li, Xinyang,et al."Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and blaNDM-1 co-harboring multidrug-resistant Enterobacter asburiae".Computational and structural biotechnology journal 21(2023):3885-3893.