Effect of Chinese patent medicine Kunling Pill on endometrial receptivity: A clinical trial, network pharmacology, and animal-based study

doi:10.5582/ddt.2023.01016

科研成果详情

题名	Effect of Chinese patent medicine Kunling Pill on endometrial receptivity: A clinical trial, network pharmacology, and animal-based study
作者	Pan, Xinyao 1,2,3; Qing, Qi 1,2,3; Zhou, Jing 1,2,3; Sun, Hongmei 1,2,3; Li, Lisha 1,2,3; Cao, Wenli 4; Ye, Feijun 4; Zhu, Jun 5; Sun, Yan 1,2,3,6; Wang, Ling 1,2,3,6
发表日期	2023-08-20
发表期刊	Drug discoveries & therapeutics 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Kunling Pill apoptosis endometrial receptivity implantation
其他关键词	EMBRYO IMPLANTATION ; EXPRESSION ; ESTROGEN
摘要	Although pregnancy success rates are raised with assisted reproductive technology, it still cannot meet clinical demands. Kunling Pill (KLP), a traditional Chinese medicine, is widely used in various gynecological disorders, particularly in improving fertility and pregnancy rates. However, the underlying mechanism of how KLP affects pregnancy outcomes remains unclear. This study aimed to explore the effects and mechanisms of KLP on endometrial receptivity. Firstly, a retrospective trial was conducted to validate the efficacy of KLP on repeated implantation failure (RIF) patients. The result indicated a significant increase in the proportion of live birth in KLP group (30.56%) compared to the control group (16.89%). Secondly, network pharmacology methods predicted the active components and network targets of KLP. Endometrial receptivity is closely associated with the activation of inflammatory factors, predicting the function of KLP on the immune system. The estrogen and apoptotic signaling pathways were also highlighted in the gene ontology enrichment analysis. Thirdly, a decreased endometrial receptivity model was established by controlled ovarian hyperstimulation (COH) in female C57BL/6 mice, divided into the COH and KLP groups. Normal female mice are as control group. In vivo, KLP administration could increase endometrial thickness and the number of endometrial glands and pinopodes. In the endometrium, KLP supplementation upregulated the expressions of estrogen receptor α, progesterone receptor, endothelial nitric oxide synthase, and integrin αVβ3 in the murine uterus and reduced serum levels of estrogen and progesterone. KLP regulated the uterine immune cells and inhibited cell apoptosis in the ovary via Bcl-2/Bax/caspase-3 pathway. In conclusion, KLP administration raised the live birth rate in RIF patients to optimize medication regimens, mainly because KLP ameliorated impaired endometrial receptivity.
资助项目	Scientific and Technological Innovation Action Plan of the Shanghai Natural Science Fund [20ZR1409100]; project of the Chinese Association of Integration of Traditional and Western Medicine special foundation for Obstetrics and Gynecology-PuZheng Pharmaceutical Foundation [FCK-PZ-08]; project for hospital management of the Shanghai Hospital Association [X2021046]; clinical trial project of the Special Foundation for Healthcare Research of the Shanghai Municipal Health Commission [202150042]
出版者	INT RESEARCH & COOPERATION ASSOC BIO & SOCIO-SCIENCES ADVANCEMENT
ISSN	1881-7831
EISSN	1881-784X
卷号	17 期号:4 页码:257-269
DOI	10.5582/ddt.2023.01016
页数	13
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:001053027500001
收录类别	PUBMED ; ESCI
在线发表日期	2023-08
URL	查看原文
Pubmed记录号	37599077
通讯作者地址	[Sun, Yan;Wang, Ling]Fudan Univ, Obstet & Gynecol Hosp, Lab Reprod Immunol, 419 Fangxie Rd, Shanghai 200011, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/182462
专题	其他_附属温岭医院（温岭市第一人民医院）
通讯作者	Sun, Yan; Wang, Ling
作者单位	1.Fudan Univ, Obstet & Gynecol Hosp, Lab Reprod Immunol, Shanghai, Peoples R China; 2.Fudan Univ, Acad Integrat Med, Shanghai, Peoples R China; 3.Shanghai Key Lab Female Reprod Endocrine Related D, Shanghai, Peoples R China; 4.Zhoushan Maternal & Child Hlth Care Hosp, Reprod Med Ctr, Zhoushan, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Wenling Peoples Hosp, Dept Obstet & Gynecol, Wenling, Zhejiang, Peoples R China; 6.Fudan Univ, Obstet & Gynecol Hosp, Lab Reprod Immunol, 419 Fangxie Rd, Shanghai 200011, Peoples R China
推荐引用方式 GB/T 7714	Pan, Xinyao,Qing, Qi,Zhou, Jing,et al. Effect of Chinese patent medicine Kunling Pill on endometrial receptivity: A clinical trial, network pharmacology, and animal-based study[J]. Drug discoveries & therapeutics,2023,17(4):257-269.
APA	Pan, Xinyao., Qing, Qi., Zhou, Jing., Sun, Hongmei., Li, Lisha., ... & Wang, Ling. (2023). Effect of Chinese patent medicine Kunling Pill on endometrial receptivity: A clinical trial, network pharmacology, and animal-based study. Drug discoveries & therapeutics, 17(4), 257-269.
MLA	Pan, Xinyao,et al."Effect of Chinese patent medicine Kunling Pill on endometrial receptivity: A clinical trial, network pharmacology, and animal-based study".Drug discoveries & therapeutics 17.4(2023):257-269.