Electroacupuncture alleviates neuropathic pain caused by SNL by promoting M2 microglia polarization through PD-L1

doi:10.1016/j.intimp.2023.110764

科研成果详情

题名	Electroacupuncture alleviates neuropathic pain caused by SNL by promoting M2 microglia polarization through PD-L1
作者	Wu, Qiaoyun1,2,4; Zheng, Yujun1,2,4; Yu, Jiaying1,2,4; Ying, Xinwang1,2,4; Gu, Xiaoxue1,2,4; Tan, Qianqian1,2,4; Tu, Wenzhan1,2,4; Lou, Xinfa2; Yang, Guanhu 1,2,4; Li, Ming3; Jiang, Songhe1,2,4
发表日期	2023-10
发表期刊	International immunopharmacology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Electroacupuncture Inflammation Microglial polarization Neuropathic pain PD-L1
其他关键词	RAT MODEL ; INFLAMMATION ; INHIBITION ; ACTIVATION ; MECHANISM
摘要	As a common clinical disease, neuropathic pain is difficult to be cured with drugs. The occurrence and progression of pain is closely related to the response of spinal microglia. Aspartof the regulation of microglialactivity,PD-L1 playsacriticalrole. Loss of PD-L1 promoted the polarization of M1-like microglia. Increased expression of PD-L1 promoted M2-like polarization. Electroacupuncture has a significant analgesic effect in clinical practice, but its specific mechanism remains to be further explored. In this study, we verified the role of PD-L1 in EA analgesia and the underlying molecular mechanism through spinal nerve ligation (SNL) in rats and lipopolysaccharide (LPS)-treated BV2 microglial cells. Forbehavioralstudiesofrats,mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured, and spinal cord neuros were examined under transmission electron microscopyto determine changes to their myelin structure. The expression levels of PD-L1 and M1/M2-specific markers in rat spinal cord and BV2 microglial cells were measured by enzyme-linked immunosorbent assay, flow cytometry, immunofluorescence staining and Western blot analysis. Our study showed that EA increased the pain threshold, reduced the destruction of myelin structure, promoted the expression of PD-L1 and PD-1, inhibited the MAPK signaling pathway, and promoted the conversion of microglial polarization from the M1 phenotype to the M2 phenotype in SNL rats. PD-L1 knockdown reversed these effects of EA. In addition, PD-L1 knockdown activated the MAPK signaling pathway, promoted microglial polarization to the M1 phenotype, decreased the expression of anti-inflammatory mediators and increased the expression of proinflammatory factors in LPS-stimulated BV2 microglial cells. Our results showed that EA may regulate the excitability of primary afferent neurons through PD-L1 and then inhibit the MAPK signaling pathway to promote the transformation of activated M1 microglia into M2 microglia, reduce inflammatory reactions, and finally achieve analgesic effects. A therapy targeting PD-L1 may be an effective strategy for treating neuropathic pain.
资助项目	National Natural Science Foundation of China; [81873376]
出版者	ELSEVIER
ISSN	1567-5769
EISSN	1878-1705
卷号	123
DOI	10.1016/j.intimp.2023.110764
页数	15
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
WOS记录号	WOS:001058899900001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-08
URL	查看原文
PubMed ID	37573685
SCOPUSEID	2-s2.0-85167831746
通讯作者地址	[Jiang, Songhe]Department of Physical Medicine and Rehabilitation,The Second Affiliated Hospital and Yuying Children's Hospital,China-USA Institute for Acupuncture and Rehabilitation,Wenzhou Medical University,268 Xue Yuan Xi Road,Wenzhou City,325027,China
Scopus学科分类	Immunology and Allergy;Immunology;Pharmacology
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/182426
专题	第二临床医学院，附属第二医院、育英儿童医院温州医科大学基础医学院（机能实验教学中心）
通讯作者	Jiang, Songhe
作者单位	1.Department of Physical Medicine and Rehabilitation,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 2.Integrative & Optimized Medicine Research Center,China-USA Institute for Acupuncture and Rehabilitation,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 3.School of Basic Medical Science,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 4.The Wenzhou Key Laboratory for Rehabilitation Research,The Provincial Key Laboratory for Acupuncture and Rehabilitation in Zhejiang Province,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 温州医科大学
通讯作者单位	附属第二医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Wu, Qiaoyun,Zheng, Yujun,Yu, Jiaying,et al. Electroacupuncture alleviates neuropathic pain caused by SNL by promoting M2 microglia polarization through PD-L1[J]. International immunopharmacology,2023,123.
APA	Wu, Qiaoyun., Zheng, Yujun., Yu, Jiaying., Ying, Xinwang., Gu, Xiaoxue., ... & Jiang, Songhe. (2023). Electroacupuncture alleviates neuropathic pain caused by SNL by promoting M2 microglia polarization through PD-L1. International immunopharmacology, 123.
MLA	Wu, Qiaoyun,et al."Electroacupuncture alleviates neuropathic pain caused by SNL by promoting M2 microglia polarization through PD-L1".International immunopharmacology 123(2023).