Puerarin acts on the skeletal muscle to improve insulin sensitivity in diabetic rats involving mu-opioid receptor

doi:10.1016/j.ejphar.2017.10.033

科研成果详情

题名	Puerarin acts on the skeletal muscle to improve insulin sensitivity in diabetic rats involving mu-opioid receptor
作者	Chen, Xiufang1,2; Wang, Lei 1; Fan, Shanshan 1; Song, Shiyu 1; Min, Haiyan 1; Wu, Yongzheng 1; He, Xuan 1; Liang, Qiao 1; Wang, Yong 3; Yi, Long 1; Gao, Qian 1
发表日期	2018-01-05
发表期刊	EUROPEAN JOURNAL OF PHARMACOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Puerarin mu-opioid receptor Skeletal muscle Insulin resistance Diabetes
其他关键词	LOWER PLASMA-GLUCOSE ; HIGH-FAT DIET ; BETA-ENDORPHIN ; SECRETORY DYSFUNCTION ; RESISTANCE ; OBESITY ; CELLS ; MICE ; ACTIVATION ; EXPRESSION
摘要	Puerarin, a major active isoflavone extracted from the root of Pueraria lobate, significantly increases plasma beta-endorphin and insulin levels and improves impaired insulin signaling in diabetic animals. However, the target tissues and underlying mechanisms in and through which puerarin functions to ameliorating insulin resistance remains largely unclear. In this study, we showed that puerarin enhanced mu-opioid receptor expression and phosphorylation, and increased insulin-stimulated glucose transporter 4 translocation to the plasma membrane in the skeletal muscle of diabetic rats, which were recaptured by a direct application of puerarin in the palmitate-induced insulin-resistant L6 myotubes. Naloxone, an antagonist of mu-opioid receptor, blocked these functions of puerarin. No beta-endorphin was detected either in the muscle of diabetic rats or in the palmitate-induced insulin-resistant L6 cells. Furthermore, we presented the evidence to show the interaction between mu-opioid receptor and insulin receptor substrate 1 in the muscle tissues and cells. These results suggested that puerarin improved insulin sensitivity in the skeletal muscle at least in part by its local effects involving mu-opioid receptor function.
资助项目	Natural Science Foundation of Zhejiang Province of ChinaNatural Science Foundation of Zhejiang Province [LY13H290007]; Wenzhou Public Welfare Science and Technology Project [Y20170167]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570775]; National High Technology Research and Development Program (863 Program) of ChinaNational High Technology Research and Development Program of China [2010CB945103]
出版者	ELSEVIER SCIENCE BV
出版地	AMSTERDAM
ISSN	0014-2999
EISSN	1879-0712
卷号	818 页码:115-123
DOI	10.1016/j.ejphar.2017.10.033
页数	9
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000418522900015
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	29061371
Scopus记录号	2-s2.0-85032003558
通讯作者地址	[Yi, Long]Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University,Nanjing,210093,China
scopus学科分类	Pharmacology
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1821
专题	检验医学院（生命科学学院、生物学实验教学中心）_生物化学与分子生物学系基础医学院（机能实验教学中心）
通讯作者	Yi, Long
作者单位	1.Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University,Nanjing,210093,China; 2.Department of Biochemistry,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325035,China; 3.State Key Laboratory of Analytical Chemistry for Life Science and Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University,Nanjing,210093,China
第一作者单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Chen, Xiufang,Wang, Lei,Fan, Shanshan,et al. Puerarin acts on the skeletal muscle to improve insulin sensitivity in diabetic rats involving mu-opioid receptor[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2018,818:115-123.
APA	Chen, Xiufang., Wang, Lei., Fan, Shanshan., Song, Shiyu., Min, Haiyan., ... & Gao, Qian. (2018). Puerarin acts on the skeletal muscle to improve insulin sensitivity in diabetic rats involving mu-opioid receptor. EUROPEAN JOURNAL OF PHARMACOLOGY, 818, 115-123.
MLA	Chen, Xiufang,et al."Puerarin acts on the skeletal muscle to improve insulin sensitivity in diabetic rats involving mu-opioid receptor".EUROPEAN JOURNAL OF PHARMACOLOGY 818(2018):115-123.