Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury

doi:10.1096/fj.202300752R

科研成果详情

题名	Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury
作者	Qu, Xiaolin1,2; Hou, Xiaoxiang 1; Zhu, Kaixin 1,3; Chen, Wen 1; Chen, Kun 1; Sang, Xianzheng 1; Wang, Chenqing 1; Zhang, Yelei 1; Xu, Haoxiang 1; Wang, Junyu 1; Hou, Qibo 4; Lv, Liquan 1; Hou, Lijun 1; Zhang, Danfeng 1
发表日期	2023-09
发表期刊	FASEB journal : official publication of the Federation of American Societies for Experimental Biology 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Research Support, Non-U.S. Gov't
关键词	microglial polarization neutrophil extracellular traps sympathoexcitation traumatic brain injury
其他关键词	HMGB1 ; NEUROINFLAMMATION ; DYSAUTONOMIA ; ASTROCYTES ; MANAGEMENT ; CELLS ; STORM
摘要	Traumatic brain injury (TBI), particularly diffuse axonal injury (DAI), often results in sympathetic hyperactivity, which can exacerbate the prognosis of TBI patients. A key component of this process is the role of neutrophils in causing neuroinflammation after TBI by forming neutrophil extracellular traps (NETs), but the connection between NETs and sympathetic excitation following TBI remains unclear. Utilizing a DAI rat model, the current investigation examined the role of NETs and the HMGB1/JNK/AP1 signaling pathway in this process. The findings revealed that sympathetic excitability intensifies and peaks 3 days post-injury, a pattern mirrored by the activation of microglia, and the escalated NETs and HMGB1 levels. Subsequent in vitro exploration validated that HMGB1 fosters microglial activation via the JNK/AP1 pathway. Moreover, in vivo experimentation revealed that the application of anti-HMGB1 and AP1 inhibitors can mitigate microglial M1 polarization post-DAI, effectively curtailing sympathetic hyperactivity. Therefore, this research elucidates that post-TBI, NETs within the PVN may precipitate sympathetic hyperactivity by stimulating M1 microglial polarization through the HMGB1/JNK/AP1 pathway.
资助项目	Chenguang project of shanghai [19CG39]; Foundation for Innovative Research Groups of the National Natural Science Foundation of China [81901242, 82071363, 82271396]; Pyramid Talent Project of Shanghai Changzheng Hospital [YQ684]; Shanghai Yang Fan Project [19YF1447900]
出版者	WILEY
ISSN	0892-6638
EISSN	1530-6860
卷号	37 期号:9
DOI	10.1096/fj.202300752R
页数	16
WOS类目	Biochemistry & Molecular Biology ; Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS记录号	WOS:001042043500001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
Pubmed记录号	37534961
Scopus记录号	2-s2.0-85166440588
通讯作者地址	[Hou, Lijun]Department of Neurosurgery,Changzheng Hospital,Naval Medical University,415 Fengyang Rd,Shanghai,China ; [Zhang, Danfeng]Department of Neurosurgery,Changzheng Hospital,Naval Medical University,Shanghai,China
scopus学科分类	Biotechnology;Biochemistry;Molecular Biology;Genetics
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/181810
专题	附属第一医院_神经外科
通讯作者	Hou, Lijun; Zhang, Danfeng
作者单位	1.Department of Neurosurgery,Changzheng Hospital,Naval Medical University,Shanghai,China; 2.Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Department of Neurosurgery,The First Naval Hospital of Southern Theater Command,Zhanjiang,China; 4.College of Liberal Arts and Sciences,University of Illinois Urbana-Champaign,Urbana,United States
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Qu, Xiaolin,Hou, Xiaoxiang,Zhu, Kaixin,et al. Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury[J]. FASEB journal : official publication of the Federation of American Societies for Experimental Biology,2023,37(9).
APA	Qu, Xiaolin., Hou, Xiaoxiang., Zhu, Kaixin., Chen, Wen., Chen, Kun., ... & Zhang, Danfeng. (2023). Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 37(9).
MLA	Qu, Xiaolin,et al."Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury".FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37.9(2023).