Orientin suppresses osteoclastogenesis and ameliorates ovariectomy-induced osteoporosis via suppressing ROS production

doi:10.1002/fsn3.3516

科研成果详情

题名	Orientin suppresses osteoclastogenesis and ameliorates ovariectomy-induced osteoporosis via suppressing ROS production
作者	Zheng, Yan1,2; Wang, Xing1; Pan, Ya-Jing1; Shi, Xiao-Feng1; Yang, Lei 3; Lou, Yong-Liang1
发表日期	2023-09
发表期刊	FOOD SCIENCE & NUTRITION 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	NF-& kappa B Nrf2 pathway Orientin osteoclast osteoporosis reactive oxygen species
其他关键词	MODEL
摘要	The aberrant differentiation of osteoclasts is a key feature of the pathogenesis of osteoporosis, which has a devastating impact on human health. While the effects of Orientin (Ori) on osteoporosis, particularly on RANKL-stimulated osteoclast production and activation, remain still unclear, Ori has been found to display several biological activities, including antioxidant and anti-inflammatory. In this work, we investigated the possible pathways through which Ori suppressed RANKL-induced osteoclast development and showed for the first time that it does so. The macrophages from the bone marrow (BMMs) were cultivated and then treated with Ori after being stimulated with RANKL. Then, TRAP-positive multinucleated cells were counted, and F-actin ring analysis was used to assess Ori's impact on mature osteoclast development. In addition, dihydroethidium (DHE) staining was used to evaluate the impact of Ori on RANKL-induced reactive oxygen species (ROS). In addition, we performed western blotting and quantitative RT-PCR analysis to investigate probable causes of these downregulation effects. We discovered that Ori inhibits the creation of osteoclasts, the gene and protein expressions unique to osteoclasts, and the ROS production. By activating Nrf2 and other ROS-scavenging enzymes, Ori reduces intracellular ROS levels. The expression of the main transcription factor of osteoclast development, c-Fos, was downregulated together with NFATc1, CTSK, and NFATc2, thanks to Ori's inhibition of RANKL-induced NF-?B. Consistent with its in vitro antiosteoclastogenic action, Ori therapy in the ovariectomized (OVX) rat model was also able to restore bone mass and improve microarchitecture in the distal femurs. Together, our results demonstrate that Ori is a flavonoid molecule with therapeutic promise for bone illnesses associated with osteoclasts, such as osteoporosis.
资助项目	Traditional Chinese Medicine of Zhejiang Province Science and Technology plan project[2023010569];National Natural Science Foundation of China[82072310];
出版者	WILEY
ISSN	2048-7177
卷号	11 期号:9 页码:5582-5595
DOI	10.1002/fsn3.3516
页数	14
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
WOS记录号	WOS:001021412200001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2023-06
URL	查看原文
PubMed ID	37701239
SCOPUSEID	2-s2.0-85163682436
通讯作者地址	[Lou, Yong-Liang]Wenzhou Key Laboratory of Sanitary Microbiology,Key Laboratory of Laboratory Medicine,Ministry of Education,China,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China ; [Yang, Lei]Department of Orthopedic,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Food Science
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/181625
专题	检验医学院（生命科学学院、生物学实验教学中心）第二临床医学院，附属第二医院、育英儿童医院
通讯作者	Yang, Lei; Lou, Yong-Liang
作者单位	1.Wenzhou Key Laboratory of Sanitary Microbiology,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China; 2.Department of Endocrinology,Affiliated Yueqing Hospital,Wenzhou,China; 3.Department of Orthopedic,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Zheng, Yan,Wang, Xing,Pan, Ya-Jing,et al. Orientin suppresses osteoclastogenesis and ameliorates ovariectomy-induced osteoporosis via suppressing ROS production[J]. FOOD SCIENCE & NUTRITION,2023,11(9):5582-5595.
APA	Zheng, Yan, Wang, Xing, Pan, Ya-Jing, Shi, Xiao-Feng, Yang, Lei, & Lou, Yong-Liang. (2023). Orientin suppresses osteoclastogenesis and ameliorates ovariectomy-induced osteoporosis via suppressing ROS production. FOOD SCIENCE & NUTRITION, 11(9), 5582-5595.
MLA	Zheng, Yan,et al."Orientin suppresses osteoclastogenesis and ameliorates ovariectomy-induced osteoporosis via suppressing ROS production".FOOD SCIENCE & NUTRITION 11.9(2023):5582-5595.