ELAVL1 promotes LPS-induced endothelial cells injury through modulation of cytokine storm

doi:10.1016/j.imbio.2023.152412

科研成果详情

题名	ELAVL1 promotes LPS-induced endothelial cells injury through modulation of cytokine storm
作者	Zhou, Chaoyang 1; Luo, Yacan 2; Huang, Zhengwei 2; Dong, Fubo 1; Lin, Junliang 1; Luo, Liwen 1; Li, Xi3; Cai, Chang2; Wu, Wenzhi2
发表日期	2023-07
发表期刊	Immunobiology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Cytokine storm ELAVL1 Endothelial cells Sepsis
其他关键词	INFLAMMATORY RESPONSE ; MESSENGER-RNA ; HUR ; BINDING ; SEPSIS ; PHOSPHORYLATION ; MODEL
摘要	Sepsis is a life-threatening systemic organ dysfunction caused by the host's unregulated response to a widespread bacterial infection. Endothelial injury is a major pathophysiologic symptom of sepsis and is considered a critical factor in promoting the progression of disease severity. ELAV like RNA binding protein 1(ELAVL1) is a ubiquitously expressed RNA-binding protein that may play an important role during sepsis. Nonetheless, the molecular mechanisms of ELAVL1 on endothelial cell damage in sepsis have not been well defined. Here, we aimed to confirm the role of ELAVL1 in sepsis-induced endothelial cell damage using lipopolysaccharide (LPS)-induced zebrafish and endothelial cells (ECs) models. We found that zebrafish larvae treated with LPS exhibited systemic endothelial cell damage, mostly manifested as pericardial edema, curved tail, and impaired angiogenesis. LPS treatments also significantly induced the expression levels of inflammatory cytokines (interleukin-6 (IL-6), IL-8, and tumor necrosis factor (TNF)-α) in vivo. In vitro, we observed the increase of ELAVL1 cytoplasmic translocation with LPS treatment. Mechanistically, targeted disruption of the ELAVL1 gene decreased the expression of TNF-α, IL-6, and IL-8 during induction of sepsis and alleviated LPS-induced blood vessel injury in zebrafish. Taken together, our study indicates that ELAVL1 knockdown may alleviate sepsis-induced endothelial cells injury by suppressing cytokine storm. Our research suggests that inhibition of ELAVL1 could reduce the level of inflammatory cytokine production induced by LPS and protect against endothelial cell injury. ELAVL1 might be a potential therapeutic target to block endothelial cells injury associated with sepsis.
资助项目	Natural Science Foundation of Zhejiang Province [LY20C120001]
出版者	ELSEVIER GMBH
ISSN	0171-2985
EISSN	1878-3279
卷号	228 期号:4
DOI	10.1016/j.imbio.2023.152412
页数	8
WOS类目	Immunology
WOS研究方向	Immunology
WOS记录号	WOS:001023082400001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-06
URL	查看原文
PubMed ID	37343439
SCOPUSEID	2-s2.0-85162103386
通讯作者地址	[Cai, Chang]The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Immunology and Allergy;Immunology;Hematology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/181197
专题	附属第一医院_肝胆外科附属第一医院其他_附属康宁医院（温州市康宁医院）
通讯作者	Cai, Chang
作者单位	1.Intensive Care Unit,The People's Hospital of Yuhuan,Yuhuan,China; 2.Department of Gastroenterology and Hepatology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang Province,Wenzhou,China; 3.The Affiliated Kangning Hospital of Wenzhou Medical University,Wenzhou,China
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Zhou, Chaoyang,Luo, Yacan,Huang, Zhengwei,et al. ELAVL1 promotes LPS-induced endothelial cells injury through modulation of cytokine storm[J]. Immunobiology,2023,228(4).
APA	Zhou, Chaoyang., Luo, Yacan., Huang, Zhengwei., Dong, Fubo., Lin, Junliang., ... & Wu, Wenzhi. (2023). ELAVL1 promotes LPS-induced endothelial cells injury through modulation of cytokine storm. Immunobiology, 228(4).
MLA	Zhou, Chaoyang,et al."ELAVL1 promotes LPS-induced endothelial cells injury through modulation of cytokine storm".Immunobiology 228.4(2023).