Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay

doi:10.1016/j.jhazmat.2019.01.079

科研成果详情

题名	Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay
作者	Ji, Chenyang 1; Yan, Lu 1; Chen, Yuanchen 1; Yue, Siqing 1; Dong, Qiaoxiang2; Chen, Jiangfei2; Zhao, Meirong 1
发表日期	2019-04-15
发表期刊	JOURNAL OF HAZARDOUS MATERIALS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Polyhalogenated carbazoles 2,7-Dibromocarbazole Transgenic zebrafish Transcriptomics Cardiac teratogenic effect
其他关键词	ARYL-HYDROCARBON RECEPTOR ; POLYHALOGENATED CARBAZOLES ; SERINE-PROTEASE ; GENE-EXPRESSION ; HALOGENATED CARBAZOLES ; EMBRYONIC-DEVELOPMENT ; SEDIMENTS ; EXPOSURE ; CANCER ; DIOXIN
摘要	Polyhalogenated carbazoles (PHCZs), which have the similar structure of dioxin, have been reported ubiquitous in the environments and drawn wide concerns. However, their potential ecological and health risks are still poorly understood. Here, wildtype zebrafish embryos were used to evaluate the environmental risks of 2,7-dibromocarbazole (2,7-DBCZ), 3,6-dibromocarbazole (3,6-DBCZ), and 3,6-dichlorocarbazole (3,6-DCCZ). 2,7-DBCZ was the most toxic compound with the 96-h LC50 value of 581.8 +/- 29.3 mu g.L-1 and the EC50 value of 201.5 +/- 6.5 mu g.L-1 for pericardial edema. The teratogenic effects of 2,7-DBCZ were tested using transgenic zebrafish larvae. The transcriptomic analysis revealed that 90 genes in zebrafish expressed differently after exposure to 2,7-DBCZ, and many pathways were related to aryl hydrocarbon receptor (AhR) activation. The qRT-PCR also showed that expression levels of AhR1 and CYP1 A in zebrafish were significantly up-regulated after exposure to 2,7-DBCZ. In conclusion, 2,7-DBCZ exhibited more potent toxicity and cardiac teratogenic effects, and presented developmental toxicity partially consistent with AhR activation. Our results will be of great help to the risk assessment and regulation-making of PHCZ5. Meanwhile, further studies should be promoted to illustrate the potential mechanism between PHCZ5 and AhR in the near future.
资助项目	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21677130, 2137005, 21707121]
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	0304-3894
EISSN	1873-3336
卷号	368 页码:514-522
DOI	10.1016/j.jhazmat.2019.01.079
页数	69
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS研究方向	Engineering ; Environmental Sciences & Ecology
WOS记录号	WOS:000461270900058
收录类别	SCIE ; EI ; SCOPUS
EI入藏号	20190506452717
EI主题词	Risk assessment
URL	查看原文
Pubmed记录号	3071078
Scopus记录号	2-s2.0-85060710725
ESI高被引论文	2020-01 ; 2020-03 ; 2020-05
通讯作者地址	[Zhao, Meirong]Zhejiang Univ Technol, Coll Environm, Hangzhou 310032, Zhejiang, Peoples R China.
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/18053
专题	公共卫生与管理学院_健康与环境生态研究所
通讯作者	Zhao, Meirong
作者单位	1.Zhejiang Univ Technol, Coll Environm, Hangzhou 310032, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Inst Environm Safety & Human Hlth, Wenzhou 325035, Peoples R China
推荐引用方式 GB/T 7714	Ji, Chenyang,Yan, Lu,Chen, Yuanchen,et al. Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay[J]. JOURNAL OF HAZARDOUS MATERIALS,2019,368:514-522.
APA	Ji, Chenyang., Yan, Lu., Chen, Yuanchen., Yue, Siqing., Dong, Qiaoxiang., ... & Zhao, Meirong. (2019). Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay. JOURNAL OF HAZARDOUS MATERIALS, 368, 514-522.
MLA	Ji, Chenyang,et al."Evaluation of the developmental toxicity of 2,7-dibromocarbazole to zebrafish based on transcriptomics assay".JOURNAL OF HAZARDOUS MATERIALS 368(2019):514-522.