Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study

doi:10.1186/s12938-021-00856-w

科研成果详情

题名	Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study
作者	Weng, Jie 1; Chen, Bi 2; Xie, Mengying 1; Wan, Xinlong4; Wang, Peng 1; Zhou, Xiaoming1; Zhou, Zhiliang1; Mei, Jin 4; Wang, Liang 5; Huang, Duping6; Wang, Zhibin4; Wang, Zhiyi 1,4,7; Chen, Chan3
发表日期	2021-02-09
发表期刊	BIOMEDICAL ENGINEERING ONLINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Thyroid gland Decellularization Extracellular matrix Scaffold Organ engineering
摘要	Background Advances in regenerative medicine technologies have been strongly proposed in the management of thyroid diseases. Mechanistically, the adoption of thyroid bioengineering requires a scaffold that shares a similar three-dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to the native extracellular matrix (ECM). Methods 24 male New Zealand white rabbits were used in this experimental study. The rabbit thyroid glands were decellularized by immersion/agitation decellularization protocol. The 3D thyroid decellularization scaffolds were tested with histological and immunostaining analyses, scanning electron microscopy, DNA quantification, mechanical properties test, cytokine assay and cytotoxicity assays. Meanwhile, the decellularization scaffold were seeded with human thyroid follicular cells, cell proliferation and thyroid peroxidase were determined to explore the biocompatibility in vitro. Results Notably, through the imaging studies, it was distinctly evident that our protocol intervention minimized cellular materials and maintained the 3D spatial structure, biomechanical properties, ECM composition, and biologic cytokine. Consequently, the decellularization scaffold was seeded with human thyroid follicular cells, thus strongly revealing its potential in reinforcing cell adhesion, proliferation, and preserve important protein expression. Conclusions The adoption of our protocol to generate a decellularized thyroid scaffold can potentially be utilized in transplantation to manage thyroid diseases through thyroid bioengineering.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LQ17H010004]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81772054, 81701379]; Zhejiang Medicines Health Science and Technology Program [2016KYB189]; WenZhou Science and Technology Bureau [Y20170179, Y20160113]
出版者	BMC
出版地	LONDON
ISSN	1475-925X
EISSN	1475-925X
卷号	20 期号:1 页码:18
DOI	10.1186/s12938-021-00856-w
页数	49
WOS类目	Engineering, Biomedical
WOS研究方向	Engineering
WOS记录号	WOS:000617735400001
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20210709923763
EI主题词	Scaffolds (biology)
URL	查看原文
PubMed ID	33563294
PMC记录号	PMC7871622
SCOPUSEID	2-s2.0-85100796919
通讯作者地址	[Wang, Zhiyi]Department of Emergency Medicine and General Practice,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325027,China ; [Chen, Chan]Department of Geriatric Medicine,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Radiological and Ultrasound Technology;Biomaterials;Biomedical Engineering;Radiology, Nuclear Medicine and Imaging
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/18027
专题	第二临床医学院、附属第二医院、育英儿童医院基础医学院（机能实验教学中心）附属第二医院附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_外科学_肿瘤外科第一临床医学院（信息与工程学院）、附属第一医院_外科学_甲状腺外科其他_温州市第三临床学院（温州市人民医院）
通讯作者	Wang, Zhiyi; Chen, Chan
作者单位	1.Department of Emergency Medicine and General Practice,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325027,China; 2.Department of Surgical Oncology,Wenzhou People’s Hospital,The Wenzhou Third Clinical Institute Affiliated With Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Geriatric Medicine,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China; 4.Institute of Bioscaffold Transplantation and Immunology,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325035,China; 5.Department of Public Health,Robbins College of Health and Human Sciences,Baylor University,Waco,United States; 6.Department of Thyroid and Breast Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 7.Center for Health Assessment,Wenzhou Medical University,Wenzhou,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 附属第一医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Weng, Jie,Chen, Bi,Xie, Mengying,et al. Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study[J]. BIOMEDICAL ENGINEERING ONLINE,2021,20(1):18.
APA	Weng, Jie., Chen, Bi., Xie, Mengying., Wan, Xinlong., Wang, Peng., ... & Chen, Chan. (2021). Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study. BIOMEDICAL ENGINEERING ONLINE, 20(1), 18.
MLA	Weng, Jie,et al."Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering: a preliminary in vitro study".BIOMEDICAL ENGINEERING ONLINE 20.1(2021):18.