Electroacupuncture inhibits dendritic spine remodeling through the srGAP3-Rac1 signaling pathway in rats with SNL

doi:10.1186/s40659-023-00439-0

科研成果详情

题名	Electroacupuncture inhibits dendritic spine remodeling through the srGAP3-Rac1 signaling pathway in rats with SNL
作者	Wu, Qiaoyun1,2,4; Cai, Chenchen1,2,4; Ying, Xinwang1,2,4; Zheng, Yujun1,2,4; Yu, Jiaying1,2,4; Gu, Xiaoxue1,2,4; Tu, Wenzhan1,2,4; Lou, Xinfa2; Yang, Guanhu 1,2,4; Li, Ming3,4; Jiang, Songhe1,2,4
发表日期	2023-05-22
发表期刊	BIOLOGICAL RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Electroacupuncture Neuropathic pain Dendritic spine remodeling srGAP3 Rac1 srGAP3/Rac1
其他关键词	DIABETIC NEUROPATHIC PAIN ; CORD-INJURY ; MESSENGER-RNA ; RAC1 ; CONTRIBUTES ; EXPRESSION ; DYNAMICS ; RECEPTOR ; ACTIN ; CDC42
摘要	Previous studies have shown that peripheral nerve injury can lead to abnormal dendritic spine remodeling in spinal dorsal horn neurons. Inhibition of abnormal dendritic spine remodeling can relieve neuropathic pain. Electroacupuncture (EA) has a beneficial effect on the treatment of neuropathic pain, but the specific mechanism remains unclear. Evidence has shown that slit-robo GTPase activating protein 3 (srGAP3) and Rho GTPase (Rac1) play very important roles in dendritic spine remodeling. Here, we used srGAP3 siRNA and Rac1 activator CN04 to confirm the relationship between SrGAP3 and Rac1 and their roles in improving neuropathic pain with EA. Spinal nerve ligation (SNL) was used as the experimental model, and thermal withdrawal latency (TWL), mechanical withdrawal threshold (MWT), Western blotting, immunohistochemistry and Golgi-Cox staining were used to examine changes in behavioral performance, protein expression and dendritic spines. More dendritic spines and higher expression levels of srGAP3 were found in the initial phase of neuropathic pain. During the maintenance phase, dendritic spines were more mature, which was consistent with lower expression levels of srGAP3 and higher expression levels of Rac1-GTP. EA during the maintenance phase reduced the density and maturity of dendritic spines of rats with SNL, increased the levels of srGAP3 and reduced the levels of Rac1-GTP, while srGAP3 siRNA and CN04 reversed the therapeutic effects of EA. These results suggest that dendritic spines have different manifestations in different stages of neuropathic pain and that EA may inhibit the abnormal dendritic spine remodeling by regulating the srGAP3/Rac1 signaling pathway to alleviate neuropathic pain.
资助项目	National Natural Science Foundation of China[81873376];Basic Research Program of Wenzhou City[Y2020064];
出版者	SOC BIOLGIA CHILE
ISSN	0716-9760
EISSN	0717-6287
卷号	56 期号:1
DOI	10.1186/s40659-023-00439-0
页数	18
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:000990384800001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	372116
SCOPUSEID	2-s2.0-85159766618
通讯作者地址	[Jiang, Songhe]Department of Physical Medicine and Rehabilitation,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,268 Xue Yuan Xi Road, Zhejiang,Wenzhou,325027,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Agricultural and Biological Sciences (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/180157
专题	第二临床医学院，附属第二医院、育英儿童医院温州医科大学基础医学院（机能实验教学中心）
通讯作者	Jiang, Songhe
作者单位	1.Department of Physical Medicine and Rehabilitation,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,268 Xue Yuan Xi Road, Zhejiang,Wenzhou,325027,China; 2.Integrative and Optimized Medicine Research Center,China-USA Institute for Acupuncture and Rehabilitation,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 3.School of Basic Medical Science,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 4.The Wenzhou Key Laboratory for Rehabilitation Research,The Provincial Key Laboratory for Acupuncture and Rehabilitation in Zhejiang Province,Wenzhou,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 温州医科大学
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Wu, Qiaoyun,Cai, Chenchen,Ying, Xinwang,et al. Electroacupuncture inhibits dendritic spine remodeling through the srGAP3-Rac1 signaling pathway in rats with SNL[J]. BIOLOGICAL RESEARCH,2023,56(1).
APA	Wu, Qiaoyun., Cai, Chenchen., Ying, Xinwang., Zheng, Yujun., Yu, Jiaying., ... & Jiang, Songhe. (2023). Electroacupuncture inhibits dendritic spine remodeling through the srGAP3-Rac1 signaling pathway in rats with SNL. BIOLOGICAL RESEARCH, 56(1).
MLA	Wu, Qiaoyun,et al."Electroacupuncture inhibits dendritic spine remodeling through the srGAP3-Rac1 signaling pathway in rats with SNL".BIOLOGICAL RESEARCH 56.1(2023).