Treatment with paraquat affects the expression of ferroptosis-related genes

doi:10.1177/09603271231167585

科研成果详情

题名	Treatment with paraquat affects the expression of ferroptosis-related genes
作者	Ge, Xiaogang 1; Cai, Qiqi 2; Zhang, Sheng2; Wu, Xianlong 2; Ying, Pan 2; Ke, Jingjing2; Yang, Zhihui2
发表日期	2023-03
发表期刊	HUMAN & EXPERIMENTAL TOXICOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Paraquat bioinformatics endoplasmic reticulum stress ferroptosis
其他关键词	R PACKAGE ; ACTIVATION ; TOXICITY ; EXPOSURE ; CYCLOOXYGENASE-2 ; INFLAMMATION ; REGULATOR ; INJURY ; MODEL
摘要	ObjectiveWe aimed to explore the mechanisms underlying paraquat (PQ)-induced damage using cell lines (NCTC1469, TC-1, TCMK-1) and bioinformatic analysis of the GSE153959 dataset. Assessment of changes in the expression of ferroptosis-related genes in cellular damage due to paraquat poisoning and the important value of these genes in the pathogenesis.MethodsData were retrieved from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) related to ferroptosis were identified by Venn plots and analyzed for enrichment. Proteins encoded by these DEGs were studied for interactions. qRT-PCR and western blotting analyses of cultured cells were used to determine the expression of ferroptosis-related DEGs and their corresponding protein levels.ResultsWe identified 25 DEGs primarily involved in epidermal growth factor receptor signaling, apoptotic signaling pathways, endoplasmic reticulum (ER) stress, and ferroptosis. From these, we uncovered eight ferroptosis-related DEGs, four of which were involved in ER response and regulators of ferroptosis-Chac1 (ChaC glutathione specific gamma-glutamylcyclotransferase 1), Atf3 (activating transcription factor 3), Tfrc (transferrin receptor), and Slc7a11 (solute carrier family 7 member 11). Significant changes in mRNA and protein levels of CHAC1, ATF3, TFRC, and SLC7A11 were confirmed in PQ-exposed cells.ConclusionER stress and ferroptosis are critical for PQ-induced cell damage. CHAC1, ATF3, TFRC, and SLC7A11 are essential molecules implicated in PQ-induced ferroptosis that may serve as therapeutic targets for the amelioration of PQ poisoning.
资助项目	Taizhou Science and Technology Department[1801ky66];Health Science and Technology Project of Zhejiang Province[2020RC143,2023RC110];
出版者	SAGE PUBLICATIONS LTD
ISSN	0960-3271
EISSN	1477-0903
卷号	42
DOI	10.1177/09603271231167585
页数	11
WOS类目	Toxicology
WOS研究方向	Toxicology
WOS记录号	WOS:000994988600001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	36960817
SCOPUSEID	2-s2.0-85150904980
通讯作者地址	[Cai, Qiqi]Department of Emergency Intensive Care Unit,Huangyan Hospital Affiliated to Wenzhou Medical University,Taizhou,China
Scopus学科分类	Toxicology;Health, Toxicology and Mutagenesis
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/180155
专题	其他_附属黄岩医院（台州市第一人民医院）
通讯作者	Cai, Qiqi
作者单位	1.Vascular and Endovascular Surgery,Huangyan Hospital Affiliated to Wenzhou Medical University,Taizhou,China; 2.Department of Emergency Intensive Care Unit,Huangyan Hospital Affiliated to Wenzhou Medical University,Taizhou,China
第一作者单位	其他_附属黄岩医院（台州市第一人民医院）
通讯作者单位	其他_附属黄岩医院（台州市第一人民医院）
第一作者的第一单位	其他_附属黄岩医院（台州市第一人民医院）
推荐引用方式 GB/T 7714	Ge, Xiaogang,Cai, Qiqi,Zhang, Sheng,et al. Treatment with paraquat affects the expression of ferroptosis-related genes[J]. HUMAN & EXPERIMENTAL TOXICOLOGY,2023,42.
APA	Ge, Xiaogang., Cai, Qiqi., Zhang, Sheng., Wu, Xianlong., Ying, Pan., ... & Yang, Zhihui. (2023). Treatment with paraquat affects the expression of ferroptosis-related genes. HUMAN & EXPERIMENTAL TOXICOLOGY, 42.
MLA	Ge, Xiaogang,et al."Treatment with paraquat affects the expression of ferroptosis-related genes".HUMAN & EXPERIMENTAL TOXICOLOGY 42(2023).