Effect of lncRNA pvt1 on HBV replication in HepG2.2.15 cells, and its possible mechanism of action

doi:10.4314/tjpr.v22i4.10

科研成果详情

题名	Effect of lncRNA pvt1 on HBV replication in HepG2.2.15 cells, and its possible mechanism of action
作者	Lai, Xijie 1; Gao, Guosheng 2; Jiang, Xiunong 1; Xie, Jing 3
发表日期	2023-04
发表期刊	TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Lnc-RNA pvt1 Hepatitis B virus STAT3 mechanism
摘要	Purpose: To investigate the influence of lncRNA pvt1 on hepatitis B virus replication in HepG2.2.15 cells and the underlying mechanism of action.Methods: Concentrations of lncRNA pvt1 in HBV-positive liver cancer cell lines (HepAD38+, HepG2.2.15+, HepG2+, and HepaRG+), and 3 HBV-negative liver cancer cell lines were determined and compared. Differences in HBV DNA content, levels of HBsAg and HBeAg, and STAT3 axis signal pathway among groups were evaluated.Results: LncRNA pvt1 levels in HBV-positive hepatoma cells were significantly higher than the corresponding expression levels in HBV-negative hepatoma cells. after si-pvt1 treatment in HepG2.2.15 cells, the 3.2K HBV DNA content increased significantly (p < 0.05), and the expression levels of HBsAg and HBeAg in HepG2.2.15 cells also significantly increased. In all groups, STAT3 protein level was comparable, but p-STAT3 protein expression in si-pvt1 group was significantly reduced, relative to those in Si NC and control groups (p < 0.05).Conclusion: Levels of lncRNA pvt1 expression in HBV-positive and HBV-negative hepatoma cells differ significantly. Treatment with si-pvt1 decrease HBV DNA content in HepG2.2.15 cells through a mechanism that relates to the STAT3 axis signal pathway. This finding may be beneficial for follow-up treatment of HBV.
出版者	PHARMACOTHERAPY GROUP
ISSN	1596-5996
EISSN	1596-9827
卷号	22 期号:4 页码:783-788
DOI	10.4314/tjpr.v22i4.10
页数	6
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000981271700010
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85164595648
通讯作者地址	[Xie, Jing]Department of Laboratory Medicine,Huangyan Hospital of Wenzhou Medical University,Taizhou First People's Hospital,Taizhou,China
Scopus学科分类	Pharmaceutical Science;Pharmacology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/180154
专题	其他_附属黄岩医院（台州市第一人民医院）
通讯作者	Xie, Jing
作者单位	1.Department of Liver Disease,Ningbo No. 2 Hospital,Ningbo,China; 2.Department of Clinical Laboratory,Ningbo No. 2 Hospital,Ningbo,China; 3.Department of Laboratory Medicine,Huangyan Hospital of Wenzhou Medical University,Taizhou First People's Hospital,Taizhou,China
通讯作者单位	其他_附属黄岩医院（台州市第一人民医院）
推荐引用方式 GB/T 7714	Lai, Xijie,Gao, Guosheng,Jiang, Xiunong,et al. Effect of lncRNA pvt1 on HBV replication in HepG2.2.15 cells, and its possible mechanism of action[J]. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH,2023,22(4):783-788.
APA	Lai, Xijie, Gao, Guosheng, Jiang, Xiunong, & Xie, Jing. (2023). Effect of lncRNA pvt1 on HBV replication in HepG2.2.15 cells, and its possible mechanism of action. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, 22(4), 783-788.
MLA	Lai, Xijie,et al."Effect of lncRNA pvt1 on HBV replication in HepG2.2.15 cells, and its possible mechanism of action".TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH 22.4(2023):783-788.