Long Non-Coding RNA TMPO-AS1 Promotes Malignant Behaviors of Hepatocellular Carcinoma Cells via YWHAG/ERK Pathway

doi:10.1166/sam.2023.4436

科研成果详情

题名	Long Non-Coding RNA TMPO-AS1 Promotes Malignant Behaviors of Hepatocellular Carcinoma Cells via YWHAG/ERK Pathway
作者	Xie, Xiaoxiao1,2,3; Qin, Le3,4; Dong, Fenglei 2,3; Zhu, Xiaoli 1
发表日期	2023-03
发表期刊	SCIENCE OF ADVANCED MATERIALS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	HCC TMPO-AS1 ERK YWHAG Migration Invasion
其他关键词	GENE-EXPRESSION ; CANCER ; PROLIFERATION ; PROGRESSION ; MIGRATION ; BIOMARKERS ; INVASION ; GROWTH
摘要	Increasing attention has been paid to long noncoding RNAs (lncRNAs) as they have been implicated in the tumorigenesis of many malignancies, such as hepatocellular carcinoma (HCC). The current research explored the pro-tumorigenic biological function of the lncRNA, TMPO-AS1, in HCC, and uncovered the underlying mech-anism. The correlations between TMPO-AS1 expression and clinical outcome in HCC were analyzed based on GEO and KM plotter databases. The differential expression of TMPO-AS1 between normal liver and HCC tissues/cells was analyzed by real-time quantitative PCR. HepG2 and Huh7 cell lines were exploited in this study. The protein expression levels were analyzed by Western blot. Cell viability was determined by CCK-8 and colony formation experiments; while cell migratioand invasion capacities were observed by wound healing IP: 203.8.109.20 On : Thu, 11 May 2023 08:15:06 and Transwell, respectively. RNA pulldown and RIP assay were adopted to determine the interaction between Copyright: American Scientific Publishers TMPO-AS1 and YWHAG. The TMPO-AS1 expression in HCC tissues was significantly higher than that in the Delivered by Ingenta normal tissues, and positively correlated with the tumor grade and poor prognosis in HCC patients. The ERK signaling pathway could be activated by overexpressing TMPO-AS1 but repressed by silencing TMPO-AS1 in HCC cells. Mechanically, TMPO-AS1 directly interacted with YWHAG and up-regulated its protein expres-sion by inhibiting its ubiquitination. The effects causing by TMPO-AS1 overexpression and silence on the cell proliferation, migration, invasion, and EMT process of HCC cells could be blocked by YWHAG silence and over expression, respectively. TMPO-AS1 may facilitate malignant behaviors of HCC cells by YWHAG/ERK pathway.
出版者	AMER SCIENTIFIC PUBLISHERS
ISSN	1947-2935
EISSN	1947-2943
卷号	15 期号:3 页码:349-360
DOI	10.1166/sam.2023.4436
页数	12
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS研究方向	Science & Technology - Other Topics ; Materials Science ; Physics
WOS记录号	WOS:000993116100002
收录类别	SCIE
通讯作者地址	[Zhu, Xiaoli]Soochow Univ, Dept Intervent Radiol, Affiliated Hosp 1, Suzhou 215006, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/180131
专题	附属第二医院_放射科附属第二医院_小儿外科第二临床医学院，附属第二医院、育英儿童医院
通讯作者	Zhu, Xiaoli
作者单位	1.Soochow Univ, Dept Intervent Radiol, Affiliated Hosp 1, Suzhou 215006, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 2, Dept Radiol, Wenzhou 325027, Peoples R China; 3.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 2, Dept Pediat Surg, Wenzhou 325027, Peoples R China
第一作者单位	附属第二医院_放射科; 第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Xie, Xiaoxiao,Qin, Le,Dong, Fenglei,et al. Long Non-Coding RNA TMPO-AS1 Promotes Malignant Behaviors of Hepatocellular Carcinoma Cells via YWHAG/ERK Pathway[J]. SCIENCE OF ADVANCED MATERIALS,2023,15(3):349-360.
APA	Xie, Xiaoxiao, Qin, Le, Dong, Fenglei, & Zhu, Xiaoli. (2023). Long Non-Coding RNA TMPO-AS1 Promotes Malignant Behaviors of Hepatocellular Carcinoma Cells via YWHAG/ERK Pathway. SCIENCE OF ADVANCED MATERIALS, 15(3), 349-360.
MLA	Xie, Xiaoxiao,et al."Long Non-Coding RNA TMPO-AS1 Promotes Malignant Behaviors of Hepatocellular Carcinoma Cells via YWHAG/ERK Pathway".SCIENCE OF ADVANCED MATERIALS 15.3(2023):349-360.