Autophagy maintains the integrity of endothelial barrier in LPS-induced lung injury

doi:10.1002/jcp.25928

科研成果详情

题名	Autophagy maintains the integrity of endothelial barrier in LPS-induced lung injury
作者	Zhang, Dan1; Zhou, Jian 2; Ye, Le Chi 3; Li, Jing 2; Wu, Zhenzhou1; Li, Yuping1; Li, Chichi4
发表日期	2018-01
发表期刊	JOURNAL OF CELLULAR PHYSIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	acute lung injury autophagy lipopolysaccharide microvascular barrier
其他关键词	RESPIRATORY-DISTRESS-SYNDROME ; LIPOPOLYSACCHARIDE ; PROTECTS ; PERMEABILITY ; ACTIVATION ; APOPTOSIS ; LIVER ; MODEL
摘要	Understanding the role and underlying regulation mechanism of autophagy in lipopolysaccharide-induced lung injury (LPS-LI) may provide potentially new pharmacological targets for treatment of acute lung injury. The aim of this study was to investigate the functional significance of autophagy in LPS-LI. The autophagy of human pulmonary microvascular endothelial cells (HPMVECs) and mice was inhibited before they were challenged with LPS. In vitro, permeability, vitality, and the LDH release rate of the cells were detected, the zonula occluden-1 (ZO-1) expression and the stress fiber formation were determined. In vivo, the lung injury was assessed. We found LPS caused high permeability and increased lactate dehydrogenase (LDH) release rate, lowered viability of the cells, inhibited the ZO-1 expression and induced stress fiber formation, these effects were further aggravated by prohibiting the level of autophagy. Consistently, in in vivo experiments, LPS-induced serious lung injury, which was reflected as edema, leukocyte infiltration and hemorrhage in lung tissue, and the high concentration of pro-inflammation cytokines tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta in bronchoalveolar lavage fluid (BALF). Inhibiting autophagy further exacerbated LPS-LI. It appears that autophagy played a protective role in LPS-LI in part through restricting the injury of lung microvascular barrier.
资助项目	Zhejiang Natural Science FoundationNatural Science Foundation of Zhejiang Province [LQ16H010002]; science and technology planning project of Wenzhou [Y20160221]
出版者	WILEY
出版地	HOBOKEN
ISSN	0021-9541
EISSN	1097-4652
卷号	233 期号:1 页码:688-698
DOI	10.1002/jcp.25928
页数	11
WOS类目	Cell Biology ; Physiology
WOS研究方向	Cell Biology ; Physiology
WOS记录号	WOS:000411829600059
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28328069
SCOPUSEID	2-s2.0-85019379515
通讯作者地址	[Li, Yuping]Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,China
Scopus学科分类	Physiology;Clinical Biochemistry;Cell Biology
引用统计	被引频次：50[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17939
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_外科学_整形外科
通讯作者	Li, Yuping
作者单位	1.Department of Respiratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,China; 2.Department of Respiratory Medicine,Zhongshan Hospital,Fudan University,Shanghai,China; 3.Department of Oncological Surgery,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,China; 4.Department of Plastic Surgery,The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang,Wenzhou City,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhang, Dan,Zhou, Jian,Ye, Le Chi,et al. Autophagy maintains the integrity of endothelial barrier in LPS-induced lung injury[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2018,233(1):688-698.
APA	Zhang, Dan., Zhou, Jian., Ye, Le Chi., Li, Jing., Wu, Zhenzhou., ... & Li, Chichi. (2018). Autophagy maintains the integrity of endothelial barrier in LPS-induced lung injury. JOURNAL OF CELLULAR PHYSIOLOGY, 233(1), 688-698.
MLA	Zhang, Dan,et al."Autophagy maintains the integrity of endothelial barrier in LPS-induced lung injury".JOURNAL OF CELLULAR PHYSIOLOGY 233.1(2018):688-698.