Polyacrylic acid-coated nanoparticles loaded with recombinant tissue plasminogen activator for the treatment of mice with ischemic stroke

doi:10.1016/j.bbrc.2019.06.079

科研成果详情

题名	Polyacrylic acid-coated nanoparticles loaded with recombinant tissue plasminogen activator for the treatment of mice with ischemic stroke
作者	Huang, Lijie1,2; Wang, Jixian 3; Huang, Shengwei 1,2; Siaw-Debrah, Felix 1,2; Nyanzu, Mark 1,2; Zhuge, Qichuan1,2
发表日期	2019-08-20
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Tissue plasminogen activator Magnetic nanoparticle Middle cerebral artery occlusion Thrombolysis
其他关键词	CLOT ; THROMBOLYSIS ; BIOCOMPATIBILITY ; TRANSPORT
摘要	Nanoparticle-based thrombolysis is a potential new treatment for stroke. The aim of this study was to investigate the efficacy of targeted thrombolysis using recombinant tissue plasminogen activator (rtPA). The rtPA was covalently bound to magnetic nanoparticles (MNP) and maintained at the target site using an external magnet. Polyacrylic acid (PAA)-coated MNP were synthesized and rtPA was then bound to the resultant PAA-MNP via carbodiimide-mediated amide bonds. For the in vitro tests, blood clots were formed in plastic centrifuge tubes with anti-coagulated plasma, thrombin and calcium chloride. For the in vivo tests, mice with ferric chloride-induced distal middle cerebral artery occlusion were treated with phosphate-buffered saline (PBS), MNP, rtPA, or MNP-rtPA (n = 6 mice per group). The binding efficacy was 80.7 +/- 1.5 mu g rtPA bound to 1 mg PAA-MNP. In the in vitro tests, the mean lysis percentage dramatically increased from 1.28% in the MNP group without rotation to 77.40% in the rtPA + MNP group with rotating magnetic field. The lysis efficiency of MNP-rtPA was 273 +/- 1.3%, and it increased to 42.8 +/- 2.8% with magnetic field rotation. The mean sizes of the infarct areas of the PBS, MNP, rtPA, and MNP-rtPA mouse groups were 20.09 +/- 6.07, 18.28 +/- 2.69, 8.65 +/- 3.63 and 4.40 +/- 2.46 mm(3), respectively. Thus, targeted MNP-rtPA accelerated thrombolysis and reduced the infarct area in a mouse model of cerebral embolism. This approach may serve as a feasible and effective treatment for embolic cerebral ischemia. (C) 2019 Elsevier Inc. All rights reserved.
资助项目	Wenzhou City Science and Technology Project [Y20150042]; Zhejiang Health Science and Technology Project [2016RCA022]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81771262, 81820108011]
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0006-291X
EISSN	1090-2104
卷号	516 期号:2 页码:565-570
DOI	10.1016/j.bbrc.2019.06.079
页数	6
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000476575500037
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	31235258
SCOPUSEID	2-s2.0-85067444811
通讯作者地址	[Huang, Lijie]Department of Neurosurgery,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Biophysics;Biochemistry;Molecular Biology;Cell Biology
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17919
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_老化与神经疾病重点实验室
通讯作者	Huang, Lijie
作者单位	1.Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Rehabilitation,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Huang, Lijie,Wang, Jixian,Huang, Shengwei,et al. Polyacrylic acid-coated nanoparticles loaded with recombinant tissue plasminogen activator for the treatment of mice with ischemic stroke[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,516(2):565-570.
APA	Huang, Lijie, Wang, Jixian, Huang, Shengwei, Siaw-Debrah, Felix, Nyanzu, Mark, & Zhuge, Qichuan. (2019). Polyacrylic acid-coated nanoparticles loaded with recombinant tissue plasminogen activator for the treatment of mice with ischemic stroke. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 516(2), 565-570.
MLA	Huang, Lijie,et al."Polyacrylic acid-coated nanoparticles loaded with recombinant tissue plasminogen activator for the treatment of mice with ischemic stroke".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 516.2(2019):565-570.