Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells

doi:10.1016/j.ygyno.2007.01.046

科研成果详情

题名	Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells
作者	Zhu, Xue-Qiong ; Lv, Jie-Qiang ; Lin, Yi ; Xiang, Mei ; Gao, Bao-Hui ; Shi, Yi-Fu
发表日期	2007-06
发表期刊	GYNECOLOGIC ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	chemokines CCL5 CCL11 mast cells uterine smooth muscle tumors
其他关键词	DIFFERENTIAL-DIAGNOSIS ; CHYMASE ; ANGIOGENESIS ; CANCER ; PROGNOSIS ; TRYPTASE ; ADENOCARCINOMA ; SUBPOPULATIONS ; ASSOCIATION ; CHEMOTAXIS
摘要	Objective. Smooth muscle tumors of uterus have been reported to contain considerable number of mast cells, especially cellular leiomyoma. However, to our knowledge the mechanism by which mast cells increased in them is not known. The purpose of this study was to reveal the different mast cell subsets in smooth muscle tumors of uterus and to investigate the mechanism of local increase of mast cells. Method. Tissue sections from 85 uterine smooth muscle tumors were studied using immunohistochemical double labeling techniques, including 40 cases of ordinary leiomyomas, 30 cases of cellular leiomyomas and 15 cases of leiomyosarcomas. The sections were double immunostained for mast cell tryptase and chymase, mast cell tryptase and ki-67, mast cell tryptase and chemokines (i.e., CCL2, CCL5, CCL11, TGF beta), as well as tryptase and CCR3. Results. MCTC-type of mast cells was the predominant type in ordinary leiomyoma and cellular leiomyoma, whereas MCT-type was seldom found in them. There was no MCC in smooth muscle tumors. The total intratumoral number of mast cells in cellular leiomyoma group was significantly higher than that in both leiomyosarcoma and ordinary leiomyoma (P<0.01). Mast cells proliferation was rarely detected in smooth muscle tumors, as revealed by constant negative labeling of the proliferation marker Ki-67 in mast cells. Almost all mast cells (tryptase positive) in smooth muscle tumors were also CCL2, CCL5, CCL11 and TGF beta positive. Expressions of CCL5 and CCL11 in tumor cells in cellular leiomyoma were all significantly higher than that in both ordinary leiomyoma and leiomyosarcoma (P<0.01). While the expression of TGF beta in tumor cells in cellular leiomyoma was not significantly different from that in ordinary leiomyoma, expression of CCL2 was not observed in smooth muscle tumor cells. There were positive correlations between CCL5 and the number of mast cells (r(s) = 0.801, P<0.01) and between CCL11 and the number of mast cells (r(s) = 0.744, P<0.01) in smooth muscle tumors as well. The vast majority of the mast cells in cellular leiomyoma were CCR3 positive. Conclusions. Using the monoclonal anti-mast cell tryptase antibody could detect all mast cells in smooth muscle tumor. The increased intratumoral mast cell counts in cellular leiomyoma might be the result of mast cells recruitment from the peripheral blood rather than local mast cells proliferation. CCL5 and CCL11, which are expressed by smooth muscle tumor cells, are possibly responsible for the recruitment of mast cells in uterine cellular leiomyoma. Whether they combine to CCR3 expressed by mast cells need further study. (c) 2007 Elsevier Inc. All rights reserved.
资助项目	Natural Science Foundation of Zhejiang Province[2005QN015];
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0090-8258
EISSN	1095-6859
卷号	105 期号:3 页码:650-656
DOI	10.1016/j.ygyno.2007.01.046
页数	7
WOS类目	Oncology ; Obstetrics & Gynecology
WOS研究方向	Oncology ; Obstetrics & Gynecology
WOS记录号	WOS:000246815700015
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	17368523
SCOPUSEID	2-s2.0-34248349109
通讯作者地址	[Zhu, Xue-Qiong]Second Affiliated Hosp, Wenzhou Med Coll, Dept Obstet & Gynecol, 109 Xueyuan Xi Rd, Wenzhou 325000, Zhejiang, Peoples R China.
TOP期刊	TOP期刊
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17869
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院_病理学基础医学院（机能实验教学中心）_病理学与病理生理学系_病理学
通讯作者	Zhu, Xue-Qiong
作者单位	1.Second Affiliated Hosp, Wenzhou Med Coll, Dept Obstet & Gynecol, Wenzhou 325000, Zhejiang, Peoples R China; 2.Second Affiliated Hosp, Wenzhou Med Coll, Dept Pathol, Wenzhou 325000, Peoples R China; 3.Zhejiang Univ, Dept Obstet & Gynecol, Womens Hosp, Sch Med, Hangzhou 310006, Peoples R China
通讯作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Zhu, Xue-Qiong,Lv, Jie-Qiang,Lin, Yi,et al. Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells[J]. GYNECOLOGIC ONCOLOGY,2007,105(3):650-656.
APA	Zhu, Xue-Qiong, Lv, Jie-Qiang, Lin, Yi, Xiang, Mei, Gao, Bao-Hui, & Shi, Yi-Fu. (2007). Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells. GYNECOLOGIC ONCOLOGY, 105(3), 650-656.
MLA	Zhu, Xue-Qiong,et al."Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells".GYNECOLOGIC ONCOLOGY 105.3(2007):650-656.