Comparative study of 3D reconstruction of rat sciatic nerve microvessels using Evans blue and lead oxide

doi:10.1179/1743132814Y.0000000394

科研成果详情

题名	Comparative study of 3D reconstruction of rat sciatic nerve microvessels using Evans blue and lead oxide
作者	Zhu, Zhaowei 1; He, Bo 1; Mao, Yihua2; Zhao, Lina2; Zhou, Xiang 1; Zhu, Qingtang 1; Zhu, Jiakai 1; Tang, Maolin2; Liu, Xiaolin 1
发表日期	2014-11
发表期刊	NEUROLOGICAL RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Evans blue Sprague-Dawley Three-dimensional reconstruction Angiography Lead oxide Peripheral nerve Sciatic nerve Intraneural microvessels Micro-computed tomography Fluorography
其他关键词	MICRO-CT ; MICROVASCULATURE
摘要	Background: The blood supply of peripheral nerve grafts is one of the important factors that affect nerve regeneration. Many investigators have studied how intraneural microvessels are distributed and ways to promote the angiogenesis of grafts. However, there still does not exist an ideal intraneural microvascular model. The purpose of this study was to compare the three-dimensional (3D) reconstruction of microvessels of the sciatic nerve in Sprague-Dawley (SD) rats after systemic perfusion with Evans blue (EB) or lead oxide. Methods: Ten adult SD rats were randomized to a fluorography group (EB) or radiography group (lead oxide). After administration of the perfusion agents, imaging information was obtained by fluorescence microscopy and micro-computed tomography (mu CT). Three-dimensional reconstruction was performed, and the diameter of microvessels at a constant distance (a cross-section was taken every 1 mm), the vascular index, and volume were measured. Two-dimensional (2D) images were obtained by serial sectioning and mu CT scanning using the two methods described. Results: In the EB group, the diameter, vascular area, and vascular index of microvessels were 11.79 +/- 7.23 mm, 0.14 +/- 0.05 mm(2), and 24.19 +/- 5.03%, respectively, and in the lead oxide group 26.45 +/- 11.81 mm, 0.06 +/- 0.02 mm(2), and 10.73 +/- 2.06%, respectively. Microvessels with diameters,20 mm were visualized better in the EB group than in the lead oxide group (P, 0.01). However, there was no significant difference between the groups in the visualization of microvessels with diameters 20-49 mu m (P > 0.05). Conclusions: Both EB and lead oxide can be used for 2D study of intraneural microvessels and 3D observation after reconstruction. Lead oxide is easy to use, and though its resolution is lower than that of EB for smaller microvessels with diameters <20 mu m, it is more suitable for studying a large sample volume.
资助项目	National High Technology Research and Development Program of China (863 Program)National High Technology Research and Development Program of China [2012AA020507]; 985 Program of Sun Yat-sen University [900353283312]; Specialized Research Fund for the Doctoral Program of Higher EducationSpecialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20120171120075]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [S201204006336]
出版者	TAYLOR & FRANCIS LTD
出版地	ABINGDON
ISSN	0161-6412
EISSN	1743-1328
卷号	36 期号:11 页码:992-1000
DOI	10.1179/1743132814Y.0000000394
页数	9
WOS类目	Clinical Neurology ; Neurosciences
WOS研究方向	Neurosciences & Neurology
WOS记录号	WOS:000342504200009
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	24846708
SCOPUSEID	2-s2.0-84918841901
通讯作者地址	[Tang, Maolin]Department of Anatomy,Wenzhou Medical College,Wenzhou,325000,China
Scopus学科分类	Neurology;Neurology (clinical)
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17826
专题	温州医科大学
通讯作者	Tang, Maolin
作者单位	1.Department of Orthopaedic and Microsurgery,The First Affiliated Hospital of Sun Yat-sen University,Guangdong,China; 2.Department of Anatomy,Wenzhou Medical College,Zhejiang,China
通讯作者单位	温州医科大学
推荐引用方式 GB/T 7714	Zhu, Zhaowei,He, Bo,Mao, Yihua,et al. Comparative study of 3D reconstruction of rat sciatic nerve microvessels using Evans blue and lead oxide[J]. NEUROLOGICAL RESEARCH,2014,36(11):992-1000.
APA	Zhu, Zhaowei., He, Bo., Mao, Yihua., Zhao, Lina., Zhou, Xiang., ... & Liu, Xiaolin. (2014). Comparative study of 3D reconstruction of rat sciatic nerve microvessels using Evans blue and lead oxide. NEUROLOGICAL RESEARCH, 36(11), 992-1000.
MLA	Zhu, Zhaowei,et al."Comparative study of 3D reconstruction of rat sciatic nerve microvessels using Evans blue and lead oxide".NEUROLOGICAL RESEARCH 36.11(2014):992-1000.