Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy

doi:10.2147/IJN.S99376

科研成果详情

题名	Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy
作者	Zhao, Ying-Zheng1,2; Zhang, Ming 1; Tian, Xin-Qiao 3; Zheng, Lei4; Lu, Cui-Tao1,2
发表日期	2016-12-31
发表期刊	INTERNATIONAL JOURNAL OF NANOMEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	diabetic cardiomyopathy prevention bFGF-loaded liposome targeted delivery
其他关键词	MICROBUBBLE DESTRUCTION ; NANOPARTICLES ; PROMOTES ; SURVIVAL ; DELIVERY ; THERAPY ; HEART ; MODEL
摘要	Basic fibroblast growth factor (bFGF)-loaded liposome (bFGF-lip) combined with ultrasound-targeted microbubble destruction (UTMD) technique was investigated to prevent diabetic cardiomyopathy (DCM). Cardiac function and myocardial ultrastructure were assessed. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) staining, immunohistochemistry staining, and Western blot assay were used to investigate the signal pathway underlying the expression of bFGF in DCM treatment. From Mason staining and TUNEL staining, bFGF-lip + UTMD group showed significant differences from the diabetes group and other groups treated with bFGF or bFGF-lip. The diabetes group showed similar results (myocardial capillary density, collagen volume fraction, and cardiac myocyte apoptosis index) to other bFGF treatment groups. Indexes from transthoracic echocardiography and hemodynamic evaluation also proved the same conclusion. These results confirmed that the abnormalities including diastolic dysfunctions, myocardial fibrosis, and metabolic disturbances could be suppressed by the different extents of twice-weekly bFGF treatments for 12 consecutive weeks (free bFGF or bFGF-lip +/- UTMD), with the strongest improvements observed in the bFGF-lip + UTMD group. The group combining bFGF-lip with UTMD demonstrated the highest level of bFGF expression among all the groups. The bFGF activated the PI3K/AKT signal pathway, causing the reduction of myocardial cell apoptosis and increase of microvascular density. This strategy using bFGF-lip and UTMD is a potential strategy in early intervention of DCM in diabetes.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81360195, 81301982, 81571392,, 81272160]; Zhejiang Provincial Foundation for Health Department [2015ZDA023]; Wenzhou Bureau of Science and Technology [Y20140726]
出版者	DOVE MEDICAL PRESS LTD
出版地	ALBANY
ISSN	1178-2013
EISSN	1178-2013
卷号	11 页码:675-686
DOI	10.2147/IJN.S99376
页数	12
WOS类目	Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
WOS记录号	WOS:000370317600001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	26937188
PMC记录号	PMC4762584
SCOPUSEID	2-s2.0-84975747920
通讯作者地址	[Lu, Cui-Tao]Department of Ultrasonography,The Second Affiliated Hospital,Wenzhou Medical University,Wenzhou City,325000,China
Scopus学科分类	Biophysics;Bioengineering;Biomaterials;Pharmaceutical Science;Drug Discovery;Organic Chemistry
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17703
专题	药学院（分析测试中心）附属第二医院附属第一医院第二临床医学院、附属第二医院、育英儿童医院第二临床医学院、附属第二医院、育英儿童医院_影像医学与核医学_超声科
通讯作者	Lu, Cui-Tao
作者单位	1.College of Pharmaceutical Sciences,Wenzhou Medical University,Zhengzhou City,China; 2.Department of Ultrasonography,The Second Affiliated Hospital,Wenzhou Medical University,Wenzhou,China; 3.Department of Ultrasonic Diagnosis,Henan Provincial People’s Hospital,Zhengzhou City,China; 4.Department of Ultrasonography,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China
第一作者单位	温州医科大学; 附属第二医院
通讯作者单位	附属第二医院
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Zhao, Ying-Zheng,Zhang, Ming,Tian, Xin-Qiao,et al. Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2016,11:675-686.
APA	Zhao, Ying-Zheng, Zhang, Ming, Tian, Xin-Qiao, Zheng, Lei, & Lu, Cui-Tao. (2016). Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy. INTERNATIONAL JOURNAL OF NANOMEDICINE, 11, 675-686.
MLA	Zhao, Ying-Zheng,et al."Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy".INTERNATIONAL JOURNAL OF NANOMEDICINE 11(2016):675-686.