Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

doi:10.3892/mmr.2017.6644

科研成果详情

题名	Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments
作者	Sun, Chaofan 1; Liu, Fen 2; Cen, Shendan 3; Chen, Lijiao1; Wang, Yi 4; Sun, Hao 1; Deng, Hui3; Hu, Rongdang1
发表日期	2017-07
发表期刊	MOLECULAR MEDICINE REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	periodontal ligament cell tensile strength cytokines osteogenesis interleukin-1 beta tumor necrosis factor-alpha
其他关键词	MESENCHYMAL STEM-CELLS ; TUMOR-NECROSIS-FACTOR ; ALKALINE-PHOSPHATASE ACTIVITY ; IN-VITRO ; GENE-EXPRESSION ; TNF-ALPHA ; MECHANICAL-STRESS ; GINGIVAL TISSUE ; DIFFERENTIATION ; INDUCTION
摘要	The present study aimed to investigate the role of orthodontic force in osteogenesis differentiation, matrix deposition and mineralization in periodontal ligament cells (PDLCs) cells in inflammatory microenvironments. The mesenchymal origin of PDLCs was confirmed by vimentin and cytokeratin staining. PDLCs were exposed to inflammatory cytokines (5 ng/ml IL-1 beta and 10 ng/ml TNF-alpha) and/or tensile strength (0.5 Hz, 12% elongation) for 12, 24 or 48 h. Cell proliferation and tensile strength-induced cytokine expression were assessed by MTT assay and ELISA, respectively. Runt-related transcription factor 2 (RUNX2) and type I collagen (COL-I) expression were analysed by reverse transcription-quantitative polymerase chain reaction and western blot analysis. Additionally, alkaline phosphatase activity was measured, and the mineralization profile was evaluated by alizarin red S staining. PDLCs exposed to tensile strength in inflammatory microenvironments exhibited reduced proliferation and mineralization potential. Treatment with the inflammatory cytokines IL-1 beta and TNF-alpha increased RUNX2 expression levels; however, decreased COL-I expression levels, indicating that bone formation and matrix deposition involve different mechanisms in PDL tissues. Notably, RUNX2 and COL-I expression levels were decreased in PDLCs exposed to a combination of an inflammatory environment and loading strength. The decreased osteogenic potential in an inflammatory microenvironment under tensile strength suggests that orthodontic force may amplify periodontal destruction in orthodontic patients with periodontitis.
资助项目	National Natural Science Foundation of China[2015KYA149];
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1791-2997
EISSN	1791-3004
卷号	16 期号:1 页码:666-672
DOI	10.3892/mmr.2017.6644
页数	7
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000405074100088
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28560407
PMC记录号	PMC5482070
SCOPUSEID	2-s2.0-85020715788
通讯作者地址	[Deng, Hui]Department of Periodontics,School of Stomatology,Wenzhou Medical University,373 West Xueyuan Road,Wenzhou, Zhejiang,325027,China
Scopus学科分类	Biochemistry;Molecular Medicine;Molecular Biology;Genetics;Oncology;Cancer Research
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17567
专题	口腔医学院、附属口腔医院_口腔正畸基础医学院（机能实验教学中心）
通讯作者	Deng, Hui
作者单位	1.Department of Orthodontics,School of Stomatology,Wenzhou Medical University,373 West Xueyuan Road,Wenzhou, Zhejiang,325027,China; 2.Department of Histology and Embryology,School of Basic Medical Sciences,China; 3.Department of Periodontics,School of Stomatology,Wenzhou Medical University,373 West Xueyuan Road,Wenzhou, Zhejiang,325027,China; 4.Faculty of Dentistry,University of Hong Kong,Hong Kong,999077,Hong Kong
第一作者单位	口腔医学院、附属口腔医院
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Sun, Chaofan,Liu, Fen,Cen, Shendan,et al. Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments[J]. MOLECULAR MEDICINE REPORTS,2017,16(1):666-672.
APA	Sun, Chaofan., Liu, Fen., Cen, Shendan., Chen, Lijiao., Wang, Yi., ... & Hu, Rongdang. (2017). Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments. MOLECULAR MEDICINE REPORTS, 16(1), 666-672.
MLA	Sun, Chaofan,et al."Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments".MOLECULAR MEDICINE REPORTS 16.1(2017):666-672.