SH3 domain-binding kinase 1 promotes proliferation and inhibits apoptosis of cervical cancer via activating the Wnt/β-catenin and Raf/ERK1/2 signaling pathways

doi:10.1002/mc.23552

科研成果详情

题名	SH3 domain-binding kinase 1 promotes proliferation and inhibits apoptosis of cervical cancer via activating the Wnt/β-catenin and Raf/ERK1/2 signaling pathways
作者	Chen, Xin1; Sun, Zhengwei1; Zhou, Shengjie 2; Jiang, Wenxiao 1; Li, Jieyi1; Song, Gendi1; Zhu, Xueqiong1,2
发表日期	2023-08
发表期刊	Molecular carcinogenesis 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Raf/ERK1/2 pathway SBK1 apoptosis cervical cancer proliferation
其他关键词	LIPID-METABOLISM ; MAPK ; BIOMARKERS ; LNCRNA ; GENES
摘要	SH3 domain-binding kinase 1 (SBK1), is a member of the serine/threonine protein kinases family, and was confirmed to be upregulated in cervical cancer in our previous study. Nonetheless, the role of SBK1 in regulating cancer occurrence and development is unclear. In this study, the stable SBK1-knockdown and -overexpressed cell models were constructed by plasmid transfection technology. Cell viability and growth were assessed through CCK-8, colony formation, and BrdU methods. Cell cycle and apoptosis were analyzed by flow cytometry. The JC-1 staining assay was used to explore mitochondrial membrane potential. The scratch and Transwell assays were used to evaluate the cell metastatic ability. The nude mice models were utilized to explore the SBK1 expression affecting tumor growth in vivo. Our research indicated a high expression of SBK1 both in tissues and cells of cervical cancer. The proliferative, migratory, as well as invasive capacities of cervical cancer cells, were suppressed, and apoptosis was enhanced after SBK1 silence, whereas SBK1 upregulation led to opposite results. In addition, Wnt/β-catenin and Raf/ERK1/2 pathways were activated by SBK1 upregulation. Furthermore, downregulation of c-Raf or β-catenin, reversed the proliferation promotion and apoptosis inhibition effects in SBK1-overexpressed cells. The same results were observed with the use of the specific Raf inhibitor. SBK1 overexpression also contributed to tumor growth in vivo. Overall, SBK1 played a vital role in cervical tumorigenesis via activating the Wnt/β-catenin and Raf/ERK1/2 pathways.
资助项目	Key Projects of Wenzhou Science and Technology Bureau[ZY2022012];Zhejiang Key Laboratory of Traditional Chinese Medicine for Diagnosis and Treatment of Gynecological Cancers[2022-11]
出版者	WILEY
ISSN	0899-1987
EISSN	1098-2744
卷号	62 期号:8 页码:1147-1162
DOI	10.1002/mc.23552
页数	16
WOS类目	Biochemistry & Molecular Biology ; Oncology
WOS研究方向	Biochemistry & Molecular Biology ; Oncology
WOS记录号	WOS:000979574900001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-05
URL	查看原文
PubMed ID	37132991
SCOPUSEID	2-s2.0-85158110644
通讯作者地址	[Zhu, Xueqiong]Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Wenzhou Medical University,No.109 Xueyuan Xi Rd, Zhejiang,Wenzhou,325027,China
Scopus学科分类	Molecular Biology;Cancer Research
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/175224
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院第一临床医学院（信息与工程学院）、附属第一医院_妇产科学
通讯作者	Zhu, Xueqiong
作者单位	1.Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Department of Obstetrics and Gynecology,Taizhou Women and Children's Hospital of Wenzhou Medical University,Zhejiang,Taizhou,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 妇产科
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 妇产科
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Chen, Xin,Sun, Zhengwei,Zhou, Shengjie,et al. SH3 domain-binding kinase 1 promotes proliferation and inhibits apoptosis of cervical cancer via activating the Wnt/β-catenin and Raf/ERK1/2 signaling pathways[J]. Molecular carcinogenesis,2023,62(8):1147-1162.
APA	Chen, Xin., Sun, Zhengwei., Zhou, Shengjie., Jiang, Wenxiao., Li, Jieyi., ... & Zhu, Xueqiong. (2023). SH3 domain-binding kinase 1 promotes proliferation and inhibits apoptosis of cervical cancer via activating the Wnt/β-catenin and Raf/ERK1/2 signaling pathways. Molecular carcinogenesis, 62(8), 1147-1162.
MLA	Chen, Xin,et al."SH3 domain-binding kinase 1 promotes proliferation and inhibits apoptosis of cervical cancer via activating the Wnt/β-catenin and Raf/ERK1/2 signaling pathways".Molecular carcinogenesis 62.8(2023):1147-1162.