Nuciferine improves random skin flap survival via TFEB-mediated activation of autophagy-lysosomal pathway

doi:10.1016/j.intimp.2023.110204

科研成果详情

题名	Nuciferine improves random skin flap survival via TFEB-mediated activation of autophagy-lysosomal pathway
作者	Xue, Kaikai1,2; Zhang, Guojian1,2; Zhou, Yiwei3; Wang, Kangyan2; Yao, Zhe1,2; Chen, Jinghao2; Zhang, Yu 2; Li, Zihao 1,2; Li, Zi2; Zheng, Zimin 2,4; Feng, Yongzeng2; Mao, Cong2; Lin, Cai1; Xia, Weidong1
发表日期	2023-06
发表期刊	International immunopharmacology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Angiogenesis Autophagy-lysosomal pathway Oxidative stress Random skin flaps TFEB
其他关键词	OXIDATIVE STRESS ; NETWORK PHARMACOLOGY ; MECHANISMS ; PROMOTES ; MTOR
摘要	Due to their simplicity and reliability, random-pattern skin flaps are commonly utilized in surgical reconstruction to repair cutaneous wounds. However, the post-operative necrosis frequently happens because of the ischemia and high-level of oxidative stress of random skin flaps, which can severely affect the healing outcomes. Earlier evidence has shown promising effect of Nuciferine (NF) on preventing hydrogen peroxide (H2O2)-induced fibroblast senescence and ischemic injury, however, whether it can function on promoting ischemic flap survival remains unknown. In this work, using network pharmacology analysis, it was possible to anticipate the prospective targets of NF in the context of ischemia. The results revealed that NF treatment minimized H2O2-induced cellular dysfunction of human umbilical vein endothelial cells (HUVECs), and also improved flap survival through strengthening angiogenesis and alleviating oxidative stress, inflammation and apoptosis in vivo. These outcomes should be attributed to TFEB-mediated enhancement of autophagy-lysosomal degradation via the AMPK-mTOR signaling pathway, whilst the restriction of autophagy stimulation with 3MA effectively diminished the above advantages of NF treatment. The increased nuclear translocation of TFEB not only restored lysosome function, but also promoted autophagosome-lysosome fusion, eventually restoring the inhibited autophagic flux and filling the high energy levels. The outcomes of our research can provide potent proof for the application of NF in the therapy of vascular insufficiency associated disorders, including random flaps
资助项目	Zhejiang Provincial Basic Public Welfare Research Program[LGD22H070001,LQ23H110002,LTGY23H150004,LY19H060003,LY22H150004];Medical Health Science and Technology Project of Zhejiang Province[2022PY069,2023KY146];
出版者	ELSEVIER
ISSN	1567-5769
EISSN	1878-1705
卷号	119
DOI	10.1016/j.intimp.2023.110204
页数	14
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
WOS记录号	WOS:000992578500001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-04
URL	查看原文
PubMed ID	37126988
SCOPUSEID	2-s2.0-85154614178
通讯作者地址	[Mao, Cong]Zhejiang Provincial Key Laboratory of Orthopedics,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,325027,China
Scopus学科分类	Immunology and Allergy;Immunology;Pharmacology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/175210
专题	附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Mao, Cong
作者单位	1.Department of Burn,Wound Repair and Regenerative Medicine Center,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Zhejiang Provincial Key Laboratory of Orthopedics,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,325027,China; 3.Department of Traditional Chinese Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 4.The Second School of Medicine,Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Xue, Kaikai,Zhang, Guojian,Zhou, Yiwei,et al. Nuciferine improves random skin flap survival via TFEB-mediated activation of autophagy-lysosomal pathway[J]. International immunopharmacology,2023,119.
APA	Xue, Kaikai., Zhang, Guojian., Zhou, Yiwei., Wang, Kangyan., Yao, Zhe., ... & Xia, Weidong. (2023). Nuciferine improves random skin flap survival via TFEB-mediated activation of autophagy-lysosomal pathway. International immunopharmacology, 119.
MLA	Xue, Kaikai,et al."Nuciferine improves random skin flap survival via TFEB-mediated activation of autophagy-lysosomal pathway".International immunopharmacology 119(2023).